Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

MushroomMan Mycology
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy

Jump to first unread post Pages: < First | | 790 | 791 | 792 | 793 | 794 | 795 | 796 | 797 | 798 | 799 | 800 | 801 | 802 | 803 | 804 | 805 | 806 | 807 | 808 | 809 | 810 | | Last >
Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #27903393 -

Nice slants SirPsycho!  And thanks for posting that song LotKid!  I feel so good now!


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: BagOfDicks]
    #27903408 -

Anyone watch this video where the transfers wasn't what he expected:



:mushroom2:


--------------------


Mushroom Cultivation Techniques...

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Grogu]
    #27903477 -

shroomsurf said:
Anyone watch this video where the transfers wasn't what he expected:



:mushroom2:


I have a question about this video. When the agar is stuck to the scalpel, he uses the lid to knock it off. Is this good sterile procedure?


--------------------
Tweeq’s wood lovers | Recipes | Moopers nats notes | Moopers subtrop notes | Milkboy gummies

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: BlueAndOrange]
    #27903483 -

I like the timpelapse of the one on red agar. You can see it growing weirdly due to the red dye #3

I wouldn't use the lid cause it'd add a possible contam vector. Better off practicing your technique to get transfers off the blade by practicing how you get them onto it.


--------------------

Links For Beginners And Beyond:mspoil:

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: BlueAndOrange]
    #27903485 -

BlueAndOrange said:
shroomsurf said:
Anyone watch this video where the transfers wasn't what he expected:



:mushroom2:


I have a question about this video. When the agar is stuck to the scalpel, he uses the lid to knock it off. Is this good sterile procedure?


I saw the same thing.
No that is not a good practice.


--------------------

   

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Egon_Spengler]
    #27903493 -

Thanks. That was my gut reaction. I have always avoided that.


--------------------
Tweeq’s wood lovers | Recipes | Moopers nats notes | Moopers subtrop notes | Milkboy gummies

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Grogu]
    #27903513 -

Interesting video none the less.. I was definitely surprised with how plate 1 turned out.. it has me thinking about adding more diversity to some of my transfers to see what happens... I don't think I'd have ever taken a transfer from that first spot...

I also liked how he numbered his transfers so he could go back and see where each one came from... I've seen someone do this before, but I didn't really see the practical application for it...

Thanks for sharing the video shroomsmurf.. I quite enjoyed it..

Too bad he didn't put that last one on one of the blue plates... I bet it would have done pretty well.


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27903568 -

Rotnpins said:
Interesting video none the less.. I was definitely surprised with how plate 1 turned out.. it has me thinking about adding more diversity to some of my transfers to see what happens... I don't think I'd have ever taken a transfer from that first spot...

I also liked how he numbered his transfers so he could go back and see where each one came from... I've seen someone do this before, but I didn't really see the practical application for it...

Thanks for sharing the video shroomsmurf.. I quite enjoyed it..

Too bad he didn't put that last one on one of the blue plates... I bet it would have done pretty well.


I've been watching so many video's...lol

Is red the only evil dye color or purple too?

:mushroom2:


--------------------


Mushroom Cultivation Techniques...

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Grogu] * 1
    #27903573 -

Erythrosine is the red dye #3 so any color that's made using that color wouldn't be a good choice either. RGB is the base color palette. Anything from the GB spectrum is fine.


--------------------

Links For Beginners And Beyond:mspoil:

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #27903596 -

hm i really liked that video - thanks for sharing
always interesting to see myc grow in different ways - ive seen cultures go from tomentose to rhizo and back to tomentose :shrug:


--------------------
helpful links
my FH build

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #27903656 -

rumfor69 said:
Erythrosine is the red dye #3 so any color that's made using that color wouldn't be a good choice either. RGB is the base color palette. Anything from the GB spectrum is fine.


lol...that is so funny, I ordered all colors but since you mentioned just blue/green I had these made for me...





20 for $20 delivered... Maybe some day I'll do the golden teacher method in this video



but for now I'm happy...

:mushroom2:


--------------------


Mushroom Cultivation Techniques...

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) *DELETED* [Re: Tormato]
    #27904395 -

Post deleted by PsyloFreak

Reason for deletion: Unknown HTML Error


--------------------
R.I.P. Dad - I will love you and keep you in my heart and memories always.
01/26/46 - 04/10/22

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: PsyloFreak] * 2
    #27904880 -

PDA clone tissue first plate




--------------------
You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: cozmyc]
    #27905376 -

When using agar to "clean up" multi-spore sources, how long do you let each transfer plate grow and what is reasonable regarding how many transfers to make before going to grain?  Asking because I've seen people suggest immediately going to another plate when the myc is no larger than say, a quarter, and I've also seen folks wait until the myc is almost taking the entire plate before doing another transfer.  Also, if T1 looks clean, is there any reason to take it to T2 or further?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Spicolis_Dad]
    #27905386 -

Spicolis_Dad said:
When using agar to "clean up" multi-spore sources, how long do you let each transfer plate grow and what is reasonable regarding how many transfers to make before going to grain?  Asking because I've seen people suggest immediately going to another plate when the myc is no larger than say, a quarter, and I've also seen folks wait until the myc is almost taking the entire plate before doing another transfer.  Also, if T1 looks clean, is there any reason to take it to T2 or further?


Best to transfer from a germ plate as soon as there's growth so as to get it before anything else overtakes it. Then you let that T1 grow out more if it's looking clean. Even if the T1 plate looks perfect your going to goto many more plates from that one to expand the culture family tree wider. Plus going to T3 or T4 plates before grain gets you a little further away from pure multispore genetics.


--------------------

Links For Beginners And Beyond:mspoil:

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #27905422 -

Thanks for the quick response.

In the specific case of Ps. Natalensis, where the genetics, in my opinion are much closer to wild, than most actives that have been cultured for so long, and there appear to be so many phenotypes,do you recommend the same? 
rumfor69 said:
Spicolis_Dad said:
When using agar to "clean up" multi-spore sources, how long do you let each transfer plate grow and what is reasonable regarding how many transfers to make before going to grain?  Asking because I've seen people suggest immediately going to another plate when the myc is no larger than say, a quarter, and I've also seen folks wait until the myc is almost taking the entire plate before doing another transfer.  Also, if T1 looks clean, is there any reason to take it to T2 or further?


Best to transfer from a germ plate as soon as there's growth so as to get it before anything else overtakes it. Then you let that T1 grow out more if it's looking clean. Even if the T1 plate looks perfect your going to goto many more plates from that one to expand the culture family tree wider. Plus going to T3 or T4 plates before grain gets you a little further away from pure multispore genetics.


Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Spicolis_Dad] * 1
    #27905430 -

I don't have experience with Nats to answer that confidently. I imagine yes, I'd do the same. But I'm not sure. It seems like Just a good standard technique approach for all varieties really.


--------------------

Links For Beginners And Beyond:mspoil:

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #27905891 -

Jack Frost T1:


Tidal Wave clone that I've been trying to clean up.. I've got another sample on water agar just incase..


Same TW culture... I think these are still enmeshed with something 🤔  what do you guys think?


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27906266 -

So I just couldn’t help it cracked it open and smelled sour guess it was bacteria.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: buddzy]
    #27906358 -

I found these on the big A site. Would they be good for agar work? Lid and cup are both pp5 so I'm assuming they are. What's the best tek for these? I was thinking pasty's but was also thinking about putting a filter on the side so I don't need to cover each one with foil.
Here's a link if you want to check them out on big A site. Hopefully I'm not violating any rules by posting a link, if so let me know I'll remove it.
Augshy Storage Containers for Slime, 50 Pack Foam Ball Storage Containers with Lids (2 oz) https://a.co/d/2jllH7Q

And for those who don't like clicking on links.



--------------------
Pastywhyte’s Ez dialed monotubs
My Miraculix Potency Test Results
The Hitchhiker's Guide to the Shroomery
My Museum
REV'S Tested: Dehydration temperature vs potency





Extras: Filter Print Post Top
Jump to top Pages: < First | | 790 | 791 | 792 | 793 | 794 | 795 | 796 | 797 | 798 | 799 | 800 | 801 | 802 | 803 | 804 | 805 | 806 | 807 | 808 | 809 | 810 | | Last >

Shop: North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* buying agar - help sinisha 3,839 6 03/31/06 07:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 8,395 8 02/14/05 09:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 7,018 8 02/25/05 01:03 PM
by agar
* test tubes agar fungophiliac 3,709 8 06/19/20 11:41 PM
by gorilliam
* LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! agentstone 5,331 12 03/30/20 12:37 PM
by Surf707
* Agar question WormholeSurfer 1,698 3 10/29/05 09:10 PM
by agar
* Agar and the global economy Dem_Bones 1,944 4 03/01/06 11:08 AM
by Dem_Bones
* Agar's Home Made Compost recipe for bulk substrates
( 1 2 3 4 all )		 agar 22,324 75 02/12/21 07:50 AM
by Bart84

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: DERRAYLD, Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
797,607 topic views. 28 members, 646 guests and 275 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.037 seconds spending 0.013 seconds on 16 queries.