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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27886178 -

Nat germ plate.. took a transfer from each of the 3 sectors between 5 o'clock and 7 o'clock.



Looks like the swab stick dropped some sort of contam that spread across the top half of the plate

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27886186 -

I don't know what I think about leaving swabs in plates. I see it work a lot times and it leave contams. I've always dabbed, streaked, or dug in to get agar on it then smear it off elsewhere.


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Sharkman] * 2
    #27886191 -

Sharkman said:
Agar is king!



how dare you assign masculinity to agar?  how do you know that's how the agar identifies? 

its freekin 2022 for crying out loud. have some couth. jeeeeeez. :rolleyes:


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #27886197 -

LotKid said:
Sharkman said:
Agar is king!



how dare you assign masculinity to agar?  how do you know that's how the agar identifies? 

its freekin 2022 for crying out loud. have some couth. jeeeeeez. :rolleyes:



Algae is asexual so I suppose it's both? Neither?


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #27886199 -

rumfor69 said:
I don't know what I think about leaving swabs in plates. I see it work a lot times and it leave contams. I've always dabbed, streaked, or dug in to get agar on it then smear it off elsewhere.




Yeah.. it's been a learning experience.. for now on I'll probably streak or cut petals and place those on the plate

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 2
    #27886211 -

I was looking through some of my older transfer plates to look for any interesting looking growth and I found these Yeti plates.

Check out how thick the ropes are on this plate!!


This ones also got some nice looking, but much thinner ropes. And it's starting to pin like crazy!


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There are only 10 types of people in the world, those that understand binary and those that do not.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #27886227 -

forgot to post pics so can anyone suggest transfer spots in the 2 plates nats and the 2 germ plates are pic tax my first fresh shroom swab to agar


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BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
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Trusted teks and linksSEED "PETRI" TEK Vent ur PC

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27886233 -

nektar61 said:
acapulcogold said:
I pull out the alcohol wipes, Clorox spray and Lysol.



mixing alcohol and clorox can produce toxic gas.

Lysol is sticky, smells horrible, and leaves a bad smell for days.

Skip clorox and lysol and just use 70% isopropyl alcohol in a plastic spray misting bottle. That's what almost all of the people doing good work here use.

70% works best, not 90%.

=-=-=-=
DJHogan said:
Just wondering if the white growth even looks like mycelium?





There's some penicillin mold growing here. The big powdery white patch turning green on the left of the left one, and the little greenish circle on the top right of the right one.

Looks like you transferred from the right place, if there's any clean to transfer from. Not sure you'll get something clean.

I'd throw these out without opening again. Once penicillin is green it's spreading spores. You do not want that in quantity like this in your home.

Work your transfer, but also start over after reading up more on basic technique. Good link to good teks in my sig line.

There's also red bacteria colonies on the far right of the right one.

Your agar looks good though. Which recipe did you use?




I should have noted that I do not mix any product ,
Each one has there place

I use the bleach on my table underneath my SAB,
And the outside of my SAB,
And the surrounding area,
Lysol over me, and alcohol wipes insides the SAB ,

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Re: AGAR ENVY! (Anything and All things agar!) [Re: wild_aussie]
    #27886238 -

wild_aussie said:
forgot to post pics so can anyone suggest transfer spots in the 2 plates nats and the 2 germ plates are pic tax my first fresh shroom swab to agar



If newcomers may apply intuition to your query then I'd choose the first plate at "10 o'clock" right inbetween the center and the outside but again I'm just getting a good vibe there...lol

#vibeshroom

:mushroom2:


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Mushroom Cultivation Techniques...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #27886278 -

rumfor69 said:
LotKid said:
Sharkman said:
Agar is king!



how dare you assign masculinity to agar?  how do you know that's how the agar identifies? 

its freekin 2022 for crying out loud. have some couth. jeeeeeez. :rolleyes:



Algae is asexual so I suppose it's both? Neither?



non- binary?


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27886392 -

nektar61 said:
DJHogan said:
Thanks for the reply, cool that it’s penicillin :grin:. The pink stuff is also very interesting looking.

My transfer is growing nice white stuff and the original plate continues to look more confidently like mycelium. No contams after 36hr but we will see.

The plates are pre poured. I would like to make my own if there is a good tek without a PC, but for now I’ll minimise my variables.

I appreciate the help and will work on some new spores :grin:



penicillin is a contam if you're growing shrooms, and it's common.

I used pre-poured for the first few runs I did that worked.

Your pre-pour plates aren't peptone are they? That's commonly offered but increases chance of contams. Is for growing bacteria in labs.

When you get clean plates with pre-poured, this agar works well for me. Also some tips there that may help anyway. You can skip the charcoal at first.
https://www.shroomery.org/forums/showflat.php/Number/27551422



Interesting. The plates are from a mushroom company so I doubt they have peptone. My house is very leaky and mouldy so I moved out of the bathroom for my transfers. So far my 5 new plates have 1 pinhead of contam that I can see, but it could still expose itself soon. The myc on the fastest is already dime-sized so I'm chuffed :grin:.

I read all your signature links, they're very useful and well written. I'm not ready to make the plunge for the PC as shipping is outrageous here. A 21L presto ends up at $330 USD haha.. However if I get deep into agar I could get a small 7 PSI one I suppose.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #27886528 -

LotKid said:
rumfor69 said:
LotKid said:
Sharkman said:
Agar is king!



how dare you assign masculinity to agar?  how do you know that's how the agar identifies? 

its freekin 2022 for crying out loud. have some couth. jeeeeeez. :rolleyes:



Algae is asexual so I suppose it's both? Neither?



non- binary?



Ooo perfect there ya go


--------------------

Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #27886988 -

Some plins. Should I grab one now or wait?
:hmm:


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You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

Edited by cozmyc (08/02/22 02:31 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cozmyc]
    #27887005 -

cozmyc said:
Some plins. Should I grab one now or wait?
:hmm:



I'd wait till they get a bit bigger and grab a couple of the biggest.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bigworm]
    #27887018 -

Sweet, thanks! Will do


--------------------
You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cozmyc]
    #27887171 -

A test dish that had too much condensation and a friend tagged along. I used a syringe that was from 2018


Edited by blazedup (08/02/22 04:32 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: blazedup]
    #27887303 -




Supposed to be copelandia or mexicana…

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #27887327 -

Trying out some no pour BRF agar with 1.5% nute. 6g per 400ml. So far nothing has been able to harbor life, but brown rice flour has been very good to me otherwise. Here’s hoping.

I randomly have very small successes on my LME. Well there’s one left, and a spore managed to germinate in exactly 1 place, where the loop accidentally scored the agar. The disk split, and there’s a little circle of growth there. It’s not growing anymore; it stalled like they all do. You think there’s a correlation there? The mycelium wanting to grow where the agar is scored?


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #27887666 -

Maybe your agar is too firm? Some brands may thicken more than others when using the same %

Edited by Seamonkey84 (08/03/22 05:19 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Seamonkey84]
    #27888936 -



This is a plate that I transferred to from germinated spores, so T2 I guess.

Is it fine to take a transfer from it now or should I wait? And also, would the next plate be ok for inoculating a jar, or do I need one more transfer?

Thanks


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