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Re: AGAR ENVY! (Anything and All things agar!) [Re: nglsnv]
    #27870103 -

it's just MEA sediment.

Edited by CowsPoopShrooms (10/14/22 06:00 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CowsPoopShrooms] * 2
    #27870107 -

I think a stick blender goes a long ways
to getting rid of sediment


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CowsPoopShrooms]
    #27870113 -

So if i mix it well, with mixer or blender next ones should be good?
Maybe it’s also cuz i store lme and agar in the fridge and without letting the temp down and mixing with hotwater ?

Edited by 0120 (07/20/22 09:55 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #27870119 -

I've only had sediment using pasty's plates perhaps from pouring too thick. I switched over to plastic plates, pour as thin as possible to cover surface, haven't had problems since.
btw I'm using LME, I mix the lme with agar powder add water, mix well with a spoon, and then just get to a boil (imo helps even out consistency)then pour into container and PC

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nndmt] * 1
    #27870123 -

The stick blender you can use as it simmer
cooks on the stove. I put water in a quart
jar, then quart jar in a pot of water, heat
it up slowly, add in LME, low simmer and
stick blend. The quart jar helps with boil
over chance and allows the stick blender
to be submerged nicely. Then i add agar,
and coloring. Then stick blend a bunch more.

Toss a grain jar lid setup on the jar
and PC 15psi 20mins, or funnel into
a media bottle, or do a no pour.


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #27870155 -

Sending the big boi x7x F2 to agar. Had enough spores that some plates look like I shook pepper on them.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27870576 -

so I had some interesting transfers from this manky dish:


these two are the best, taken from the 9 o'clock section, but look like they're enmeshed with something? random mold in there as well do you think? I think the plates are pretty clean.



really appreciate all your feedback folks, it stops the ole head getting too anxious about simple stuff, ya know?  :laugh:
:asianofapproval:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: wild_aussie] * 1
    #27870586 -

The thinner areas?

Edited by CowsPoopShrooms (10/14/22 06:00 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CowsPoopShrooms] * 1
    #27870598 -

All I see is mycelium, others may disagree. 1st pic I wouldnt take to grain though, could use a transfer or two.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CowsPoopShrooms] * 1
    #27870600 -

It kinda just looks like more sectors to me
which would make sense seems how is was so
sectoral in the last plate.


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69] * 1
    #27870772 -

I just used 2 syringes and a print to finally get back into agar work after like 5 years. Both syringes and the print are pretty old so let's see if I get any growth 🤞🏽

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Re: AGAR ENVY! (Anything and All things agar!) [Re: blazedup] * 4
    #27870778 -

Welcome back to the mycelium network


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #27870796 -

rumfor69 said:
Welcome back to the mycelium network




Thank you 🙏🏽 ....

I have a question maybe someone can answer unless I have to ask it somewhere else. Does parafilm need to be placed on the dishes in SAB?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: blazedup]
    #27870802 -

Yes bro.


--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: blazedup] * 1
    #27870804 -

I would recommend it unless you have a very steady hand. I wrapped my first set of dishes in open air, most of them got contamination along the edges when the lids wiggled

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Seamonkey84]
    #27870821 -

I wrap in front of the flow hood, but how do spores in the air work their way against gravity and get between the lid and the dish and to the agar?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: blazedup] * 2
    #27870823 -

When wrapping plastic plates, it is easy to squeeze them in the middle, which sucks in air around the edges. I unwrap and rewrap plates in the SAB, always.

Contams can be sucked in on this airflow around the edges.


--------------------
Two eyes to look, One eye to see.
Prying open my third eye

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Seamonkey84]
    #27870829 -

Guerrilla said:
Yes bro.



Seamonkey84 said:
I would recommend it unless you have a very steady hand. I wrapped my first set of dishes in open air, most of them got contamination along the edges when the lids wiggled



Thanks, I appreciate the quick responses. I will do the smart thing and SAB it.

Another question... Once it's on does that make it ok to pick them up in open air to check them out?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: blazedup]
    #27870831 -

Yes, once wrapped, they are safe to take out of Still air or flow and store


--------------------
Two eyes to look, One eye to see.
Prying open my third eye

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Re: AGAR ENVY! (Anything and All things agar!) [Re: blazedup]
    #27870832 -

Yes again, the parafilm protects the inside of the plate from contamination.

Beat to it by 7 seconds haha :wink:


--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

Edited by Guerrilla (07/20/22 07:48 PM)

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