Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: < First | | 767 | 768 | 769 | 770 | 771 | 772 | 773 | 774 | 775 | 776 | 777 | 778 | 779 | 780 | 781 | 782 | 783 | 784 | 785 | 786 | 787 | | Last >
Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #27861938 -

Wall.E said:
If I had to guess it’s because mushrooms normally grow in wet and damp environments like after a heavy rain or whatever. That’s when people find mushrooms. So while the may get hydrated, they don’t quite extend their hyphae until their enzymes or whatever detect food.



Thanks, great answer...


--------------------


Mushroom Cultivation Techniques...

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Grogu]
    #27861943 -

I made some transfers on 7/7 and this is my first time working with agar. What do you guys think? Looks clean to me except for two plates which are suspect. One that definitely has moved around from the center, took a bit for me to get it back to center once the lid was back on and the other may have moved around, I can't remember properly.

I also wonder about the 2nd last one which looks like clen growth but only coming out of half of it. Is it encountering bacteria? Doesn't look like it on top but I wonder why it is hesitant. Is it just that the culture on that side of the wedge is shit?

I was thinking to Josex Poke some to make LC or should I take more transfers?



Thank you

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Padaone]
    #27861953 -

Those are not ready for lc by any means, you need clean, uniform growth.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27861955 -

Are they not uniform because of contams or not uniform because too many different cultures?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Padaone]
    #27861959 -

The glad rounds aren't easy to see through unfortunately.

The 2nd plate looks to have growth everywhere which appears to be contamination.
The plates with growth isolated to the transfer have my likelihood of being clean.
The problem is lots of the issues could be from bacteria which is tough to see through opaque plastic.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Padaone]
    #27861962 -

If there is bacteria present in agar but mycelium will ride over it, will it always be there or does it vanish with enough transfers? There seems to be couple spots I would like to take transfers or is this plate doomed?


And also think its safe to say about this other culture, that my grains were clean enough to use for G2G?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27861971 -

DERRAYLD said:
The glad rounds aren't easy to see through unfortunately.

The 2nd plate looks to have growth everywhere which appears to be contamination.
The plates with growth isolated to the transfer have my likelihood of being clean.
The problem is lots of the issues could be from bacteria which is tough to see through opaque plastic.




Are these pictures more useful?


Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Padaone] * 1
    #27861975 -

You can see the transfer better but the surface of the agar is what I'm looking for.
Bacteria can be tough to see or identify if you're new to agar.
Any slimey growth with a slight opaque color should be seen as suspect.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27861977 -

Hmmmm. Idk if I'll be able to get better pictures. I dont see any bacteria on the plates. I had some on some of my initial innoc plates so I know basically what I'm looking for in that respect. As you say slightly opaque slimey growth, in that respect these plates seem clean. The way its growing unevenly is my main question.

The one with growth all over the shop is probably from my trying to move it to the center after transfer by shaking it.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Padaone]
    #27861983 -

If you're confident that they are clean of bacteria then the plates look okay, if that's  T1 then it is common for the growth to be irregular, you must isolate the good growth and transfer to a new plate.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27862002 -

Hi Team,

need some advice on this plate. I think it looks good but also dont know if I should do another transfer.



--------------------
:mushroom2:  psychonaut  :mushroom2:

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Planeraptor333]
    #27862004 -

That looks pretty good, I'd send it to grain but also take transfers to isolate it a bit further.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27862005 -

should I wait till it fills the plate a bit more?


--------------------
:mushroom2:  psychonaut  :mushroom2:

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27862007 -

then there is this guy.... it is getting thicker and growing but is sooo dense looking..



--------------------
:mushroom2:  psychonaut  :mushroom2:

Edited by Planeraptor333 (07/14/22 03:36 AM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Planeraptor333]
    #27862008 -

You can purely to have more mycelium to transfer but it's usable as it is.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27862009 -

DERRAYLD said:
If you're confident that they are clean of bacteria then the plates look okay, if that's  T1 then it is common for the growth to be irregular, you must isolate the good growth and transfer to a new plate.




Thank you, you've been very helpful. If I may ask just one more question based on what you see here where would you take transfers from? I know some of the pics are shoddy but I think I see good growth on some, just want to confirm.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Planeraptor333]
    #27862011 -

Planeraptor333 said:
then there is this guy.... it is getting thicker and growing but is sooo dense looking..





That looks more tormentose which can hide things fairly easily, transfer once grown out a bit more.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Padaone]
    #27862012 -

Padaone said:
DERRAYLD said:
If you're confident that they are clean of bacteria then the plates look okay, if that's  T1 then it is common for the growth to be irregular, you must isolate the good growth and transfer to a new plate.




Thank you, you've been very helpful. If I may ask just one more question based on what you see here where would you take transfers from? I know some of the pics are shoddy but I think I see good growth on some, just want to confirm.



We tend to go for rhizomorphic growth because it's far easier to identify anything piggybacking.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD] * 1
    #27862013 -

Start here



Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27862018 -

Awesome, just the two segments I was thinking.

Extras: Filter Print Post Top
Jump to top Pages: < First | | 767 | 768 | 769 | 770 | 771 | 772 | 773 | 774 | 775 | 776 | 777 | 778 | 779 | 780 | 781 | 782 | 783 | 784 | 785 | 786 | 787 | | Last >

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Simmering Karo (Agar?)
( 1 2 all )
MushroomFriend 3,686 36 12/06/04 01:45 PM
by MushroomFriend
* buying agar - help sinisha 3,821 6 03/31/06 05:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 8,342 8 02/14/05 07:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,981 8 02/25/05 11:03 AM
by agar
* test tubes agar fungophiliac 3,676 8 06/19/20 09:41 PM
by gorilliam
* LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! agentstone 5,298 12 03/30/20 10:37 AM
by Surf707
* Agar and the global economy Dem_Bones 1,903 4 03/01/06 09:08 AM
by Dem_Bones
* Agar question WormholeSurfer 1,674 3 10/29/05 07:10 PM
by agar

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
748,078 topic views. 15 members, 1,399 guests and 50 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.039 seconds spending 0.016 seconds on 15 queries.