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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
    #27823912 -

Nichrome said:
These fuckers can chew through one layer of film or cling.


That cutter looks nifty. I'll need to pick one of those up.




Nasty.. what are those, mites?

Yeah, if I switch to parafilm, I probably get the one with the cutter case.. it's a few dollars more, but it's refillable and looks like it would be convenient. Plus the hard plastic will help keep the roll from getting damaged...

Like LotKid said, sometimes you've gotta pay a little  more for quality equipment.. it really just depends on preference and how much you want to invest in the hobby

I'll probably stick with my dollar store wrap until I get my flow hood built and then upgrade to parafilm sometime after that


Edited by Rotnpins (06/17/22 10:14 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome] * 1
    #27823913 -

Ah, so grafting tape is the same thing? I didn't know this, I will have to reach out to someone that asked if grafting tape they found on Amazon meant for plants was a suitable substitute for parfilm or Saran Wrap. Thanks Nichrome :thumbup:

:themoreyouknow:


I am a little embarrassed to admit that I have always used Saran Wrap rolls cut to size because it was what I did when I first started growing years ago and it wasn't broke so I didn't fix it. I think it's finally time to step up to parafilm. :lol: :rockon:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Healing Oakland]
    #27823934 -

There is a skillset/learning curve with any new tool. Bear in mind.

Rot, yes those are mites and they exist everywhere. Even on orbital space craft. NASA has problems with them.

A lot of the time when tubs fail (mold, bacteria, etc) these are the culprit too. They are microscopic.


--------------------
Question everything, it's very responsive.
culture storage

Edited by Nichrome (06/17/22 10:19 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Healing Oakland]
    #27823935 -

I use cling film also

and here I was recently thinking, how about I just wrap plates once around, would save a fair bit of time on a large stack of plates

then I saw that picture of the mite
:epicfacepalm:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SingularFusion] * 2
    #27823947 -

That photo is inside a colonized dish. You can see all the eggs bound up in the mycelium that has been all chewed up.

This is healthy mycelium at the same magnification.


--------------------
Question everything, it's very responsive.
culture storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome] * 1
    #27823957 -

Your microscope has a camera function? NOYCE! What microscope do you use?


--------------------
Healing Oakland
An Entheogen Church Co-operative

Are you an established or aspiring cultivator in or near the Bay Area?
:pm: Healing Oakland is hiring. Shoot us a message. :pm:

This is a real place of employment with payroll, benefits, the whole nine.

Registered church with the state of CA

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
    #27823959 -

Nichrome said:
There is a skillset/learning curve with any new tool. Bear in mind.

Rot, yes those are mites and they exist everywhere. Even on orbital space craft. NASA has problems with them.

A lot of the time when tubs fail (mold, bacteria, etc) these are the culprit too. They are microscopic.



Very cool to actually see what they look like..

That makes a lot of sense... I unfortunately had a fruit fly problem pop up at the beginning of spring.. and the couple tubs that they got into contaminated shortly after.. it's one of those "chicken and the egg" situations, but if I were to guess I'd say that they played a big part in the contamination by carrying some sort of spores and transferring them into the tubs. Especially considering that 2 of the tubs were pf tek shredded to bulk, so I was pretty confident in my spawn on those tubs.

I can only imagine the sort of crap that mites can carry with them.


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27823988 -

Fruit flys, or fungus gnats? Fruit flies have a rounded silhouette like a smaller version of the common house fly. Fungus gnats feature dangling legs and long bodies that make them appear similar to a small mosquito. I'm guessing it was fungus gnats.


--------------------
Healing Oakland
An Entheogen Church Co-operative

Are you an established or aspiring cultivator in or near the Bay Area?
:pm: Healing Oakland is hiring. Shoot us a message. :pm:

This is a real place of employment with payroll, benefits, the whole nine.

Registered church with the state of CA

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Healing Oakland]
    #27824032 -

I'm pretty sure they're fruit flies because they originated in my kitchen and moved their way back to my grow area, but I could be wrong...

I ended up making a crap-ton of traps using apple cider vinegar, hanging fly tape, switching to fruiting in bags, and storing fruit differentlyin my kitchen... they're finally all gone.

If they return, I'll try and see if they were actually fruit flies or fungus gnats. Hopefully with all the new precautions I'm taking around the house they will stay gone 🤞

My poor cat jumped into one of the hanging straps of fly tape :lol: thankfully other people have had the same thing happen, so a quick Google search told me that wiping her fur with a paper towel that had vegetable oil on it would get all the sticky shit off of her.


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27824115 -

Rotnpins said:
My poor cat jumped into one of the hanging straps of fly tape :lol: thankfully other people have had the same thing happen, so a quick Google search told me that wiping her fur with a paper towel that had vegetable oil on it would get all the sticky shit off of her.


Fucking cats they cant help themselves lol, google once again saves the day


--------------------
Check your vector

Gummies by milkboy
Milkboys Public Display of Affection
I'm a Semp


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #27824153 -

First go at using a spore print from a recent flush. I was surprised at the amount of growth already. Either this is all bacteria, or are they simply new hydrated spores ready to go. I scraped a lot of spores off of the print with flamed loop cooled in agar. Probably too many in hindsight.

My question is, if this is good mycelium (is it? Hard for me to tell at this stage) should I be transferring these isolated colonies at the end of the streak before they merge with one another?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hotboxer] * 1
    #27824159 -

Looks like mycelium to me

It's all preference.. some people prefer to transfer from germ plates quickly before comtans get a chance to take hold, others prefer to let them grow out a bit.. I'd probably take some transfers and then keep the plate and let it grow out


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 1
    #27824169 -

Awesome, thanks Rotn. Sounds like a good plan.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hotboxer]
    #27824265 -

Hotboxer said:
Awesome, thanks Rotn. Sounds like a good plan.


It looks good.

I'd let it grow out 2 to 5 days so you're only transferring mycelium, not spores. And maybe you can see some mycelium performing better than other, and pick the more robust mycelium.

If you get contam I'd transfer then, away from the contam. if you want to post another photo in a few days we can give opinions on where to transfer from.

No need to transferring the isolated colonies before they merge with one another.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 3
    #27824284 -

A 4" roll of parafilm will roll 1500 plates when you use a 1"x4" section, which is just right IMO.

You can do more if you are anal and careful but a 1"x4" piece works very good and is very easy to cut off the 4" roll. Look like dis



--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420] * 1
    #27825013 -

Hey! Dats wut i does too!


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #27825112 -


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Re: First agar plate - what am i seeing? [Re: Infinite Monkeys]
    #27825530 -

When i’m doing transfers and spraying my plates with iso, notes with marker erases.. How u deal with that guys?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420] * 1
    #27825534 -

You don’t.

Why are you spraying plates?


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: First agar plate - what am i seeing? [Re: 0120]
    #27825552 -

0120 said:
When i’m doing transfers and spraying my plates with iso, notes with marker erases.. How u deal with that guys?


I recently bought some lab grade markers that don’t come off with iso. I wipe my dishes before opening them or putting them in the sterile air in front of the flow hood too.

I’m a noob and super paranoid about contam.


--------------------
Tweeq’s wood lovers | Recipes | Moopers nats notes | Moopers subtrop notes | Milkboy gummies

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Cultivation Supplies   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Substrate Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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