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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Infinite Monkeys] * 1
    #27816945 -

Go for something like this;

Tube Cutter,5~70mm Stainless Steel Tubing Cutter Heavy Duty Pipe Cutter for Copper, Aluminum And Stainless Steel Pipes https://a.co/d/djybceJ

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Infinite Monkeys] * 1
    #27816961 -

Infinite Monkeys said:
Could you share a link of the type of pipe cutter you mean? I'm struggling to find one in my location that will fit the size of a cling wrap roll


Sure! I have a 1 1/4 inch diameter pipe cutter and it is just barely big enough for a standard roll. I would recommend something like this 1 1/5 inch model or larger. The link is Amazon, but you should be able to find one at your local hardware store and probably wal-mart too, but your choices will be more limited. If you're searching elsewhere online, just search for "pvc pipe cutter"

Here's a video of a slightly more expensive model($25) in action.

Edited by JXAllen (06/13/22 03:01 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27816999 -

nektar61 said:
Screwup said:
I’ll keep that in mind. I only have ten plates so perhaps it’s time to expand that quantity.



$110 with shipping for 500 plates:
https://www.southernlabware.com/petri-dish-polystyrene-90-x-15mm-sterile-500-cs.html

Says 100 mm, but they're 90 mm, but good. Come in bags of ten.


Ty I was gonna look for that link you shared with me before hahaah

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Re: AGAR ENVY! (Anything and All things agar!) [Re: JXAllen]
    #27817164 -

JXAllen said:
Infinite Monkeys said:
Could you share a link of the type of pipe cutter you mean? I'm struggling to find one in my location that will fit the size of a cling wrap roll


Sure! I have a 1 1/4 inch diameter pipe cutter and it is just barely big enough for a standard roll. I would recommend something like this 1 1/5 inch model or larger. The link is Amazon, but you should be able to find one at your local hardware store and probably wal-mart too, but your choices will be more limited. If you're searching elsewhere online, just search for "pvc pipe cutter"

Here's a video of a slightly more expensive model($25) in action.


I haven't tried it but I bet you can cut shrink wrap rolls with a long sharp serrated knife, like maybe a good bread knife. Or a hack saw. Those are good things to have, too.

Or a hot knife, haha. But I'd do that outside with a mask on.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #27817167 -

I recently switched from LME to grain soak water agar and I’ve been getting these strange colors showing up in my plates. The strange color almost seems to be under the agar.

#1 top view 

#1 top view with backlighting
 
#1 bottom view with backlighting



#2 top view

#2 top view with backlighting

#2 bottom view with backlighting



I’ve ran these same cultures side by side with LME plates with no sign of contamination or anything unusual in their LME counterparts.

I’ve also stored some of these same GSW plates at room temperature for 2-3 weeks and not seen anything unusual until I transferred the mycelium into them

I’ve been using LME plates for years and I hardly ever get contamination when doing transfers. When I have seen contamination on plates it’s usually just a random satellite colony of mold mycelium. I started messing around with GSW agar a few weeks ago and I noticed several of my cultures instantly switch from tomentose to rhizomorphic growth. The mycelium seems happy but that strange color looks awful. Every one of my cultures has showed this growth pattern on the GSW including several cubensis, ovoids, and azurescens

Hoping to get a quick answer that this is normal with GSW even though I’ve never seen anything like this

Edited by LegionOfShroom (06/13/22 09:15 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LegionOfShroom]
    #27817200 -

The brown growth is similar to the type of growth that is commonly seen on stone producers plates.

What is your process for creating GSW agar?

To the best of my knowledge, you need to cook grain water agar for longer than LMEA.
You'll want to use grain soak water within ~24 hours.

Munchauzen, I think, stores his GSW as ice cubes.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27817342 -

I have an extremely sharp chef knife. It cuts through meat and vegetables like butter.  But even with that,  I struggled to get through the roll. You have to cut around and then through. P9 makes it look easy in that video he made. It's not, and like I said, leaves ragged edges that the film gets stuck on,  which tears it when you're trying to wrap. Any saw would make this worse.  You want a nice clean cut.  The pipe cutter is, as I said before a far easier and SAFER way to cut through rolls of cling wrap.  I'll bet money.... anyone that tries cutting one of those rolls with a knife will be looking for an easier, safer way. Anyway.... that's all I'm going to push this.  Feel free to do as you like.


--------------------
There are only 10 types of people in the world, those that understand binary and those that do not.


Edited by JXAllen (06/13/22 12:00 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: JXAllen] * 2
    #27817355 -

#parafilmGang

So much less hassle


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #27817370 -

Can confirm. Tried cutting my cling film and it was a fucking bitch. Took me over five minutes and then I busted out the hack saw just today. Made a mess.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27817388 -

I just grab these pre cut rolls with a handle from the dollar store.. they're over with the office supplies and craft stuff.




--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27817397 -

For the parafilm users, what are you doing to cut it? Are the dispensers any good? They seem super expensive and have no reviews, or the cheap ones have terrible reviews. I love parafilm, but I’d love it more if it were easier to cut a 1”x4” piece off the roll.


--------------------
Tweeq’s wood lovers | Recipes | Moopers nats notes | Moopers subtrop notes | Milkboy gummies

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BlueAndOrange] * 2
    #27817413 -

I just use scissors.  When i'm doing plate work i cut a length off the roll and cut it down into the size strips i want. So then i just have a lil pile of pre cut strips sitting next to me. And i just grab and use strips as i need them. If i end up needing more then i grab more. If i have some pre cuts left over then i use them next time i need them.


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid] * 2
    #27817429 -

:lol: this conversation again

It's like an endless loop.
:biggrin:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27817434 -

Hey all I've been looking for anything on how mycelium should grow on agar and what to look for for the best healthiest agar.  Thanks


--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
Hunter S. Thompson
"I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse
" Sticking feathers up your ass does not make you a chicken!" Tyler Durden
"He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson
The noob forum
Crack Plates
Fastfreds media cookbook 60 agar/lc recipes
Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide
Trusted teks and linksSEED "PETRI" TEK Vent ur PC

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Re: AGAR ENVY! (Anything and All things agar!) [Re: wild_aussie]
    #27817440 -

What is it you're trying to do? Go to grain, LC, transfers?


--------------------
Faht Links to Faht Grow Skills
Yoshi's cheat sheet to grains

"Alice asks the White Rabbit, 'How long is forever?' and he replies, “'Sometimes, just one second.'”

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MurphSmurf] * 1
    #27817443 -

Playing with some different media: MEA-DCP (Dicalcium phosphate), Regular MEA and CWA (Coconut water agar)





Also been playing with millet flour, my cultures are loving it. It is thick, but I don't give a shit about the most "clear" plate - just if my cultures like it.

500ml h2O - 10g Malt - 10g Millet flour - Agar (at whatever you like, pictured plates were at 10g's, I've gone as little as 4g).




Edited by MurphSmurf (06/13/22 01:17 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: wild_aussie] * 1
    #27817445 -

We all generally tend to go for rhizomorphic growth because it's easier to identify anything hiding in the agar but tormentose is also okay.

Organized growth is favorable and mostly what you are after.

Rhizomorphic



Tormentose



That's a basic idea

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27817463 -

DERRAYLD said:
:lol: this conversation again

It's like an endless loop.
:biggrin:




:tardpig::kingtard::tardpig:


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MurphSmurf]
    #27817476 -

Yes!  I am trying to do everything.  Go to grain, lc, slants, just to know wtf I'm looking for lol.




--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
Hunter S. Thompson
"I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse
" Sticking feathers up your ass does not make you a chicken!" Tyler Durden
"He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson
The noob forum
Crack Plates
Fastfreds media cookbook 60 agar/lc recipes
Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide
Trusted teks and linksSEED "PETRI" TEK Vent ur PC

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: wild_aussie]
    #27817503 -

wild_aussie said:
Hey all I've been looking for anything on how mycelium should grow on agar and what to look for for the best healthiest agar.  Thanks



This is linked in my sigline, in the "things I wish I would have known" link, but here's the direct link:

Great thread on how shroom growing should and shouldn't look, at every step.

Anyone know what happened to Tmethyl, anyway? Hasn't been here in a year and 9 months. T is cool.

Also: Stro's Cleaning and Isolating On Agar


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (06/13/22 01:58 PM)

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Shop: Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Mono Tub Substrate   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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