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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27556865 -

SpaceBaby said:
nektar61 said:
SpaceBaby said:
Alcabenzi [first] germ plate:







Groovy germ plates, SB.

Though is that penicillin...the round spot on the left of the third pic (Alcabenzi [first] germ plate)? Damn condensation, can't tell. The right side of the long swab blob should be great to transfer though.

Does anyone grow Penicillium intentionally here? Wouldn't be bad to have for the zombie apocalypse, if it could be extracted. I've thought about it.


thanks and yep, nketar61, that is mos def contam, pretty sure Penicillium. if i can safely open the plate i was thinking to tranfer from the area you mentioned.

what say?


There are people here who'd transfer away from it but I might not.

I'm no expert, but I'm more wary of molds (like Penicilliums) over bacteria....I've heard molds are more likely to spread around the room (via spores) if you open them. If you have another Alcabenzi plate without that, I'd use it instead. Or if you can grow another quick.

If not, if you open it, maybe spray some lysol around the room before and after, and use more ISO?

I would certainly wear a mask to open it, but I wear a mask when doing plates no matter what. (simple cloth mask).

What if you opened it at an angle and sprayed ISO (or h2o2) on the mold, held plate at an angle so you don't get it on the shrooms, then cut a bit of the shrooms out opposite over on the right and transfer that. Wonder if that would keep mold spores down, or not?

All opinion. Maybe someone else knows better.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (11/24/21 11:45 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27556869 -

I dont think there's any need to spray the room with anything or use extra ISO inside the SAB. If that were the only plate I had to work with then I would approach everything the same way, except I would do that plates transfers last - after all other SAB work had been done and sealed up.

My biggest concern is that it's already sporulating and would have dropped spores onto the mycelium making it more difficult to clean up.


--------------------



I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27556883 -

bigfootscreepyuncl said:
I dont think there's any need to spray the room with anything or use extra ISO inside the SAB. If that were the only plate I had to work with then I would approach everything the same way, except I would do that plates transfers last - after all other SAB work had been done and sealed up.

My biggest concern is that it's already sporulating and would have dropped spores onto the mycelium making it more difficult to clean up.



Thank you.

Do it last. I didn't think of that, but yes.

Green means it's sporulating, right?


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27556889 -

nektar61 said:
bigfootscreepyuncl said:
I dont think there's any need to spray the room with anything or use extra ISO inside the SAB. If that were the only plate I had to work with then I would approach everything the same way, except I would do that plates transfers last - after all other SAB work had been done and sealed up.

My biggest concern is that it's already sporulating and would have dropped spores onto the mycelium making it more difficult to clean up.



Thank you.

Do it last. I didn't think of that, but yes.

Green means it's sporulating, right?


To the best of my knowledge, yes, when it's green it's sporulating. I do agree with you though that if there are other plates of that variety to focus on them instead of risking further mold contamination and that bacteria is much easier to deal with. I didn't mean to come off as argumentative with my reply if I did, I was mostly agreeing with you I just had a couple things I wanted to add.


--------------------



I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27556915 -

bigfootscreepyuncl said:
nektar61 said:
bigfootscreepyuncl said:
I dont think there's any need to spray the room with anything or use extra ISO inside the SAB. If that were the only plate I had to work with then I would approach everything the same way, except I would do that plates transfers last - after all other SAB work had been done and sealed up.

My biggest concern is that it's already sporulating and would have dropped spores onto the mycelium making it more difficult to clean up.



Thank you.

Do it last. I didn't think of that, but yes.

Green means it's sporulating, right?


To the best of my knowledge, yes, when it's green it's sporulating. I do agree with you though that if there are other plates of that variety to focus on them instead of risking further mold contamination and that bacteria is much easier to deal with. I didn't mean to come off as argumentative with my reply if I did, I was mostly agreeing with you I just had a couple things I wanted to add.


When I get a swab I always rub it on 3 or 4 plates before I stab it in the last plate like he did. That way there are more choices if one goes green (or yellow, or red, or black, or clear and slimey).

If he doesn't want to risk it and that's the only one, I do see a lot of Alcabenzi floating around.

Alcabenzi = Alabama + Mexican, right?


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27557173 -

bigfootscreepyuncl said:
SpaceBaby said:
today

poured a few plates




:biggrin:


Goddamn, I want a hood so bad...


:awesantabig: when I move into my house...

So many beautiful plates!!!!


--------------------
Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx] * 1
    #27557629 -



Psilocybe subaeruginascens MS plate. I was surprised by how quickly these spores germinated. I'd hopefully like to introduce this species to the surrounding area as we don't have any naturally occurring woodloving Psilocybes.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #27557646 -

Tormato said:
verum subsequentis said:
verum subsequentis said:
"It's astonishing, it works well!"
Been tryin to tell folks for years.
#GladI'mNotRetarded
RIP Lotkid



I've been made aware that some took this as my saying Lotkid had passed. That is not the message intended. It's a throw back to a playful argument him and I had on the board a while back. He's die hard parafilm and I'm die hard glad cling. That's all.


I'm glad that was cleared up .. miss that guy! :mushroom2::heart:


I'm not famous enuff to have false death rumors.  That's like some Abe Vigoda level shit right there...

:lahey:


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid] * 2
    #27557714 -

clearly agar



2 L water
20 g agar
19 g lme
1.5 g peptone

prepped in my new tool!



--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #27557729 -

Hey buddy. Nice to "See" ya. Happy day to you.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #27557775 -

LotKid said:
I'm not famous enuff to have false death rumors.  That's like some Abe Vigoda level shit right there...

:lahey:


Now there's a sight for sore eyes! Good to see ya LotKid! :rockon:


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27557818 -



I have an addiction, it's a sickness really.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27557821 -

SpaceBaby said:
clearly agar



2 L water
20 g agar
19 g lme
1.5 g peptone

prepped in my new tool!




Does peptone help? I searched and see some people on here use it, but that it used to be used more here.

I just remember in college using it in agar to grow bacteria. haha. But does it help shroom mycelium without helping grow bacteria?

Quick search shows someone else wondering the same thing here; https://www.shroomery.org/forums/showflat.php/Number/27465895#27465895

I don't know anything about it, just curious.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27557832 -

nektar61 said:
SpaceBaby said:
clearly agar



Does peptone help? I searched and see some people on here use it, but that it used to be used more here.

I just remember in college using it in agar to grow bacteria. haha. But does it help shroom mycelium without helping grow bacteria?

Quick search shows someone else wondering the same thing here; https://www.shroomery.org/forums/showflat.php/Number/27465895#27465895

I don't know anything about it, just curious.


dunno really. i'll see how my transfers do. just poured ~100 p plates. i don't like yeast... too dirty. maybe i'll boil and strain some. usually i so straight lme and agar.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27557917 -

Could this be the start of plate pins?




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Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning]
    #27557928 -

aNewBeginning said:
Could this be the start of plate pins?







wait and see


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27557929 -

Yep. Looks like primordia


--------------------
LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning]
    #27558015 -

aNewBeginning said:
Could this be the start of plate pins?






Yes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet] * 1
    #27558032 -



a chunky plate pin



clone of a thin-at-the-bottom-fat-at-the-top Melmak, my favorite Mel phenotype.

Edited by multifractal (11/26/21 12:55 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal] * 1
    #27558092 -

T1 transfer day here :biggrin:

From these


To these


Also wanna shout out to CocaineBuffet, those Albino Blue Magnolia you did a giveaway with are insane!
Seriously outgrowing anything else I have under germ, these were streaked with the swab eight days ago... Thanks again dude :thumbup:


--------------------
MistaMonsta's LAGM 2022

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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