Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Myyco.com APE Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag

Jump to first unread post Pages: < First | | 583 | 584 | 585 | 586 | 587 | 588 | 589 | 590 | 591 | 592 | 593 | 594 | 595 | 596 | 597 | 598 | 599 | 600 | 601 | 602 | 603 | | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27556865 -

SpaceBaby said:
nektar61 said:
SpaceBaby said:
Alcabenzi [first] germ plate:







Groovy germ plates, SB.

Though is that penicillin...the round spot on the left of the third pic (Alcabenzi [first] germ plate)? Damn condensation, can't tell. The right side of the long swab blob should be great to transfer though.

Does anyone grow Penicillium intentionally here? Wouldn't be bad to have for the zombie apocalypse, if it could be extracted. I've thought about it.


thanks and yep, nketar61, that is mos def contam, pretty sure Penicillium. if i can safely open the plate i was thinking to tranfer from the area you mentioned.

what say?


There are people here who'd transfer away from it but I might not.

I'm no expert, but I'm more wary of molds (like Penicilliums) over bacteria....I've heard molds are more likely to spread around the room (via spores) if you open them. If you have another Alcabenzi plate without that, I'd use it instead. Or if you can grow another quick.

If not, if you open it, maybe spray some lysol around the room before and after, and use more ISO?

I would certainly wear a mask to open it, but I wear a mask when doing plates no matter what. (simple cloth mask).

What if you opened it at an angle and sprayed ISO (or h2o2) on the mold, held plate at an angle so you don't get it on the shrooms, then cut a bit of the shrooms out opposite over on the right and transfer that. Wonder if that would keep mold spores down, or not?

All opinion. Maybe someone else knows better.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (11/24/21 11:45 PM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27556869 -

I dont think there's any need to spray the room with anything or use extra ISO inside the SAB. If that were the only plate I had to work with then I would approach everything the same way, except I would do that plates transfers last - after all other SAB work had been done and sealed up.

My biggest concern is that it's already sporulating and would have dropped spores onto the mycelium making it more difficult to clean up.


--------------------



I :heart: 5318008


NOT a virgin!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27556883 -

bigfootscreepyuncl said:
I dont think there's any need to spray the room with anything or use extra ISO inside the SAB. If that were the only plate I had to work with then I would approach everything the same way, except I would do that plates transfers last - after all other SAB work had been done and sealed up.

My biggest concern is that it's already sporulating and would have dropped spores onto the mycelium making it more difficult to clean up.



Thank you.

Do it last. I didn't think of that, but yes.

Green means it's sporulating, right?


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27556889 -

nektar61 said:
bigfootscreepyuncl said:
I dont think there's any need to spray the room with anything or use extra ISO inside the SAB. If that were the only plate I had to work with then I would approach everything the same way, except I would do that plates transfers last - after all other SAB work had been done and sealed up.

My biggest concern is that it's already sporulating and would have dropped spores onto the mycelium making it more difficult to clean up.



Thank you.

Do it last. I didn't think of that, but yes.

Green means it's sporulating, right?


To the best of my knowledge, yes, when it's green it's sporulating. I do agree with you though that if there are other plates of that variety to focus on them instead of risking further mold contamination and that bacteria is much easier to deal with. I didn't mean to come off as argumentative with my reply if I did, I was mostly agreeing with you I just had a couple things I wanted to add.


--------------------



I :heart: 5318008


NOT a virgin!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27556915 -

bigfootscreepyuncl said:
nektar61 said:
bigfootscreepyuncl said:
I dont think there's any need to spray the room with anything or use extra ISO inside the SAB. If that were the only plate I had to work with then I would approach everything the same way, except I would do that plates transfers last - after all other SAB work had been done and sealed up.

My biggest concern is that it's already sporulating and would have dropped spores onto the mycelium making it more difficult to clean up.



Thank you.

Do it last. I didn't think of that, but yes.

Green means it's sporulating, right?


To the best of my knowledge, yes, when it's green it's sporulating. I do agree with you though that if there are other plates of that variety to focus on them instead of risking further mold contamination and that bacteria is much easier to deal with. I didn't mean to come off as argumentative with my reply if I did, I was mostly agreeing with you I just had a couple things I wanted to add.


When I get a swab I always rub it on 3 or 4 plates before I stab it in the last plate like he did. That way there are more choices if one goes green (or yellow, or red, or black, or clear and slimey).

If he doesn't want to risk it and that's the only one, I do see a lot of Alcabenzi floating around.

Alcabenzi = Alabama + Mexican, right?


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27557173 -

bigfootscreepyuncl said:
SpaceBaby said:
today

poured a few plates




:biggrin:


Goddamn, I want a hood so bad...


:awesantabig: when I move into my house...

So many beautiful plates!!!!


--------------------
Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx] * 1
    #27557629 -



Psilocybe subaeruginascens MS plate. I was surprised by how quickly these spores germinated. I'd hopefully like to introduce this species to the surrounding area as we don't have any naturally occurring woodloving Psilocybes.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #27557646 -

Tormato said:
verum subsequentis said:
verum subsequentis said:
"It's astonishing, it works well!"
Been tryin to tell folks for years.
#GladI'mNotRetarded
RIP Lotkid



I've been made aware that some took this as my saying Lotkid had passed. That is not the message intended. It's a throw back to a playful argument him and I had on the board a while back. He's die hard parafilm and I'm die hard glad cling. That's all.


I'm glad that was cleared up .. miss that guy! :mushroom2::heart:


I'm not famous enuff to have false death rumors.  That's like some Abe Vigoda level shit right there...

:lahey:


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid] * 2
    #27557714 -

clearly agar



2 L water
20 g agar
19 g lme
1.5 g peptone

prepped in my new tool!



--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #27557729 -

Hey buddy. Nice to "See" ya. Happy day to you.


--------------------
Masters and slaves hunting clones        How not to isolate (it worked, but)


Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #27557775 -

LotKid said:
I'm not famous enuff to have false death rumors.  That's like some Abe Vigoda level shit right there...

:lahey:


Now there's a sight for sore eyes! Good to see ya LotKid! :rockon:


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27557818 -



I have an addiction, it's a sickness really.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27557821 -

SpaceBaby said:
clearly agar



2 L water
20 g agar
19 g lme
1.5 g peptone

prepped in my new tool!




Does peptone help? I searched and see some people on here use it, but that it used to be used more here.

I just remember in college using it in agar to grow bacteria. haha. But does it help shroom mycelium without helping grow bacteria?

Quick search shows someone else wondering the same thing here; https://www.shroomery.org/forums/showflat.php/Number/27465895#27465895

I don't know anything about it, just curious.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27557832 -

nektar61 said:
SpaceBaby said:
clearly agar



Does peptone help? I searched and see some people on here use it, but that it used to be used more here.

I just remember in college using it in agar to grow bacteria. haha. But does it help shroom mycelium without helping grow bacteria?

Quick search shows someone else wondering the same thing here; https://www.shroomery.org/forums/showflat.php/Number/27465895#27465895

I don't know anything about it, just curious.


dunno really. i'll see how my transfers do. just poured ~100 p plates. i don't like yeast... too dirty. maybe i'll boil and strain some. usually i so straight lme and agar.


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27557917 -

Could this be the start of plate pins?




Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning]
    #27557928 -

aNewBeginning said:
Could this be the start of plate pins?







wait and see


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27557929 -

Yep. Looks like primordia


--------------------
LAGM2020

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning]
    #27558015 -

aNewBeginning said:
Could this be the start of plate pins?






Yes

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet] * 1
    #27558032 -



a chunky plate pin



clone of a thin-at-the-bottom-fat-at-the-top Melmak, my favorite Mel phenotype.

Edited by multifractal (11/26/21 12:55 AM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal] * 1
    #27558092 -

T1 transfer day here :biggrin:

From these


To these


Also wanna shout out to CocaineBuffet, those Albino Blue Magnolia you did a giveaway with are insane!
Seriously outgrowing anything else I have under germ, these were streaked with the swab eight days ago... Thanks again dude :thumbup:


--------------------
MistaMonsta's LAGM 2022

Extras: Filter Print Post Top
Jump to top Pages: < First | | 583 | 584 | 585 | 586 | 587 | 588 | 589 | 590 | 591 | 592 | 593 | 594 | 595 | 596 | 597 | 598 | 599 | 600 | 601 | 602 | 603 | | Last >

Shop: Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Myyco.com APE Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* oops drunk moment... beer + agar dont splice.. buggers 988 2 09/21/05 01:02 AM
by buggers
* agar question madsick 657 6 04/05/08 12:23 AM
by madsick
* agar
( 1 2 all )		 mikearch 2,580 21 10/05/02 03:52 PM
by mikearch
* Where to find agar around town? HUBSonDUBS 1,043 8 12/24/06 12:12 AM
by Workman
* Mycelium or Mold? on agar beknowdo 14,181 6 01/29/07 02:07 PM
by RogerRabbit
* ? re: LB-agar, also seeking the mycologist aka Erliquin Lefty 3,069 3 05/24/04 03:09 PM
by Lefty
* Agar Work Myc0Maniac 2,908 14 05/13/05 10:17 AM
by Roadkill
* Horse_Meister - Agar Attempts - Log with PHOTS - Open to Experienced Advice Horse_Meister 2,696 14 01/12/08 10:08 PM
by Blutjager

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: DERRAYLD, Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
797,423 topic views. 21 members, 822 guests and 241 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.043 seconds spending 0.017 seconds on 16 queries.