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 mikearch
ohioan
Registered: 08/21/02
Posts: 42
Last seen: 22 years, 5 months
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 agar 
#923851 - 10/01/02 07:49 PM (22 years, 5 months ago) |
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i am making about 10 agar plates and i was wondering if i could pour the agar in the plates and then sterilize the plates or do i have do sterilize the agar first then pour it in the sterilized plates?
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 StainItBlue
Registered: 09/25/02
Posts: 261
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Re: agar [Re: mikearch]
#923885 - 10/01/02 07:56 PM (22 years, 5 months ago) |
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that answer should definatley be in the FAQ .if its not just follow a tek..
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mikearch
ohioan
Registered: 08/21/02
Posts: 42
Last seen: 22 years, 5 months
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everything i read says put it all in one container, sterilize and then pour
i would like to know if i can pour then sterilize the petri dishes?
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mickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
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Re: agar [Re: mikearch]
#923932 - 10/01/02 08:09 PM (22 years, 5 months ago) |
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I always sterilize the agar and then pour it into pre-sterilized dishes.
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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five
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mikearch
ohioan
Registered: 08/21/02
Posts: 42
Last seen: 22 years, 5 months
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how would i go about pouring the agar and then sterilizing the petri dishes?
could i put the dishes in a ziplock bag and then put them in the pc?
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tripndicular
My Minds Eye IsRhizomorphic
Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
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 Re: agar [Re: mikearch]
#924651 - 10/02/02 10:34 AM (22 years, 5 months ago) |
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Sterilize the agar in PC , then pour . The results of doing it in the opposite could be quite disatorous . Plastic petris will melt (I assume you are using plastic) in PC .
You also risk not getting mixed up enough , thus not gelling properly . 
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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .
Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
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Olgualion
Shaman-In-Training
Registered: 11/13/01
Posts: 1,253
Loc: Currently Earth...
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 Re: agar [Re: mikearch]
#924739 - 10/02/02 11:21 AM (22 years, 5 months ago) |
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----deleted----
ahhh forget it
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Study the past...
See the future...
Edited by Olgualion (10/02/02 10:44 PM)
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kykeon
Dead wishes
Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 18 years, 5 months
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hey mickey, how can someone pre-sterilize the petri dishes if those are made of plastic? or is it okee withous sterilization, i mean plastic is a dead thing, no bacteria can live on it, right?
Kykeon
athens, greece
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The living ghost of Kykeon
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 9 days, 22 hours
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Re: agar [Re: kykeon]
#924772 - 10/02/02 11:35 AM (22 years, 5 months ago) |
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>i mean plastic is a dead thing, no bacteria can live on it, right?
A scalpel is a dead thing also, but do you sterilize it before use or not?
The plastic petri dishes come presterilized, so if they are originaly sealed, there?s no need to sterilize them.
Arketa. 
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kykeon
Dead wishes
Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 18 years, 5 months
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 Re: agar [Re: Anno]
#924806 - 10/02/02 11:49 AM (22 years, 5 months ago) |
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Quote:
A scalpel is a dead thing also, but do you sterilize it before use or not?
i do  i understand your point.
Quote:
The plastic petri dishes come presterilized, so if they are originaly sealed, there?s no need to sterilize them.
yeap. my petri dishes from Mycomania were in a sealed bag. once opened, bacteria are starting to attack, right? the bag has 20 petri dishes, so wtf?
also my dear greek-speaking Anno you didnt tell me how to sterilize them if needed. does this mean that after their 1st use, they have to be thrown away?
Kykeon
athens, greece
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The living ghost of Kykeon
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tripndicular
My Minds Eye IsRhizomorphic
Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
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Re: agar [Re: kykeon]
#924839 - 10/02/02 11:58 AM (22 years, 5 months ago) |
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Mine to came sterile in a sealed plastic sleeve .
Contams were no problem , but I used a make shift tent with sterile cloth , surgeons mask , lot of pinesol and clorox spray , sterile gloves .
Basically just create as sterile an enviro that you can . Open dishes only when pouring , and open as little as needed to make pour .
I poured ten dishes , innoced four , had zero contams(with exception of one on purpose contam dish ) , did my transfers after selection with no contam probs .
Good luck
Yes throw them away after use , if you cant do that , best method to clean them up is with alcohol 90% or better . But why risk contams in future , petris are cheap , unless you use glass which are very costly , these are the only ytpe you can "safely" reuse .
--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .
Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
Edited by tripndicular (10/02/02 12:03 PM)
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mickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
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Re: agar [Re: kykeon]
#925357 - 10/02/02 03:29 PM (22 years, 5 months ago) |
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In reply to:
hey mickey, how can someone pre-sterilize the petri dishes if those are made of plastic?
You don't, they should be sterile when you get them. I use glass petris now.
In reply to:
or is it okee withous sterilization, i mean plastic is a dead thing, no bacteria can live on it, right?
It's not that anything would be living in the plastic. If you opened the petri dishes and let contaminants fall into them, you would have problems. Since I use glass dishes, after I clean them out and dry them, I PC them to sterilize them.
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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 9 days, 22 hours
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Re: agar [Re: mikearch]
#926520 - 10/02/02 09:36 PM (22 years, 5 months ago) |
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kykeon
Dead wishes
Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 18 years, 5 months
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okee. is there a good internet based supplier of glass petri dishes. once i saw my plastic ones, i knew i couldnt fall in love with them; i think i need glass ones.
Kykeon
athens, greece
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The living ghost of Kykeon
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mickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
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Re: agar [Re: kykeon]
#927624 - 10/03/02 03:58 AM (22 years, 5 months ago) |
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I think for you it would be better to find a company in Europe. Shipping glass from any company in the USA to Greece would be expensive. Glass is heavy.
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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five
Edited by mickey_rourke (10/03/02 04:30 AM)
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 9 days, 22 hours
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Re: agar [Re: kykeon]
#927730 - 10/03/02 04:31 AM (22 years, 5 months ago) |
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Glass petri dishes cost around 2 euro per piece here.
Shipping for 10 dishes would be around 6 euro.
I?d send you some if you want. PM me.
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Zildjian
human
Registered: 09/11/02
Posts: 208
Loc: new zealand
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Re: agar [Re: mikearch]
#927838 - 10/03/02 05:40 AM (22 years, 5 months ago) |
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No, you cannot sterilize the plates with the agar in them.
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Servator
Viral Agent
Registered: 08/30/02
Posts: 4,893
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Re: agar [Re: Anno]
#927844 - 10/03/02 05:46 AM (22 years, 5 months ago) |
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I managed to get 70 pyrex 60mm petris for 35 dollars off ebay. They have the setup for any type of mad scientist shit you might be interested in. And as far as I know, prepouring agar before sterilizing isn't a problem if you're carefle. (never done it myself, so I can't make promises)
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 9 days, 22 hours
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Re: agar [Re: Zildjian]
#927905 - 10/03/02 06:54 AM (22 years, 5 months ago) |
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> No, you cannot sterilize the plates with the agar in them.
Sure you can.
it?s just not as good and practical as pouring them after.
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comario2
amateur
Registered: 09/06/02
Posts: 1,352
Loc: between places
Last seen: 12 years, 2 months
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Re: agar [Re: Anno]
#928070 - 10/03/02 09:13 AM (22 years, 5 months ago) |
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its messy. remember you can sterilize plastic or glass petris (and just about anything else on the planet) with ALCOHOL. its generally available everywhere, cheap, not messy and effective. i guess the only draw-back is that it is highly flammable and therefore kids should not be messing with it, particularly when they have their kitchen stoves on, doing oven teks, scalpel flame sterilizing or what not.
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comario
"crusaders against emotional poverty"
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 9 days, 22 hours
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Re: agar [Re: comario2]
#928181 - 10/03/02 10:13 AM (22 years, 5 months ago) |
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>ALCOHOL...effective.
I wouldn?t really on alcohol killing bacterial endospores.
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mikearch
ohioan
Registered: 08/21/02
Posts: 42
Last seen: 22 years, 5 months
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Re: agar [Re: Anno]
#934639 - 10/05/02 01:52 PM (22 years, 5 months ago) |
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thanks for the help everyone, i decided to just sterilize then pour, been two days since i put a drop from my syringe on each one, hopefully ill see something in the next week.
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