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Amazon Shop for: ½ Pint Jars, Agar

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Offlinebuggers
Mushroom CloudLayin'Motherfucker
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Registered: 03/20/05
Posts: 324
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oops drunk moment... beer + agar dont splice..
    #4688195 - 09/21/05 12:07 AM (11 years, 2 months ago)

^^^ unless you're using malt for making beer!

well i've been trying to get a pure strain of psilocybe cubensis... andddd....

i was playing heroes 2 with my roommate and forgot about a jar of agar i had cooling on the stove waiting for the peroxide to be added... i was so tied into the game that the jar ended up kinda solidifying on the outside...

i hurried as quick as i could to squirt some peroxide in there and pour plates (inside my still air chamber... no gloves but i washed my hands good with alcohol)

nevertheless, the plates ended up chunky... i did my transfers anyway figuring they would still gorw (the plates i transferred off of were starting to get mold in little spots... so i needed to get a transfer or i'll have to start all over again... i only have 4 cc's of spore solution left!)

will chunky plates do me any good?... by chunky i mean that there are clumps of jelled agar amongst thinner parts... ie. the surface is really uneven.

i've been getting really nice growth since i did my initial transfer from antibiotic agar to peroxidated agar but my plates started getting a little bit of contam on them... i think i got haughty because i heard tell of the wonders of peroxidated agar and that you could pour plates in an open kitchen with no contams.
silly me... back to the glovebox err... still air chamber.

ps before i could finish pouring all the plates i wanted, the agar gelled up in the quart jar i PCed it in... so i dropped a big wedge of agar in there too... do you think i could get anything out of this? the jar is sealed tite, was sterilized, and is the last bit of peroxidated agar made from 250 ml of malt dextrose agar and 3 ml of h2o2 added once the agar had nearly congealed.

pss. is this bordering on advanced? i have trouble getting agar questions answered in this forum


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InvisibleThumpaCap
Beer Taster

Registered: 02/19/05
Posts: 568
Loc: Shroomwhere Out There
Re: oops drunk moment... beer + agar dont splice.. [Re: buggers]
    #4688352 - 09/21/05 12:34 AM (11 years, 2 months ago)

:shrug:


--------------------
:bongload: Look into my heyes !! :bongload:


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Offlinebuggers
Mushroom CloudLayin'Motherfucker
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Registered: 03/20/05
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Re: oops drunk moment... beer + agar dont splice.. [Re: ThumpaCap]
    #4688462 - 09/21/05 01:02 AM (11 years, 2 months ago)

yep...  :eek:


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Amazon Shop for: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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