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InvisiblefahtsterM
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Scrimshaw] * 1
    #27562351 - 11/29/21 10:03 PM (1 month, 17 days ago)

Quote:

Quirkmeister92 said:
You reccomend rinsing the final sample before innoculating grains? Just to be safe?




Not necessarily.. that’s just what my reasoning tells me.  I’m open to hearing what y’all think about it

Quote:

Scrimshaw said:
I’ll take my chances moving a wedge from a sealed dish to a fresh jar 3 inches away before rinsing it in water first. Seems more complicated for no benefit.

If something could land on the wedge from the time the dish was open to when it went to the jar, why couldn’t something land on it after it came out of the water?




I guess I was meaning more like something getting on it while it’s in the dish during colonizing.  I just hear ppl say that “the culture appeared clean when I added it to the jar” but then the jar turns to shit.  I suppose the water could make the wedge more sticky to things in the air during the transfer.. maybe move the sterile water jar right next to the grain jar so there’s not even 3”. 

But like I said, my reasoning could be flawed.. that’s why I’d like to hear what y’all have to say about it

Edit:  if you’re usung SAB, I guess you want to be as fast as possible and adding that step would be counterproductive.. with using the oven bag, I have a bit more time to fiddle around and rinse tissue.  If you’re using a hood, there wouldn’t be a point since it’s all clean anyway.. so I guess nvm lol

Faht


Edited by fahtster (11/29/21 10:10 PM)


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InvisiblemistamonstaS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster]
    #27562513 - 11/30/21 01:12 AM (1 month, 17 days ago)

Hey guys, need a little advice as to whats going on here..

These are four days after first transfer from a very underdeveloped plate that I tried saving from penicillin. I'm not really sure what they're doing just yet and truthfully I can't be certain that last pic is even mushroom mycelium haha


And there's this Albino Blue Magnolia, looks like it had some hidden contam (bacteria?) that is taking to the plate before the ABM mycelium


Any advice appreciated :smile:


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OnlineTri-Polar
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Re: AGAR ENVY! (Anything and All things agar!) [Re: mistamonsta]
    #27562561 - 11/30/21 02:50 AM (1 month, 17 days ago)

Quote:

mistamonsta said:
And there's this Albino Blue Magnolia, looks like it had some hidden contam (bacteria?) that is taking to the plate before the ABM mycelium






as a bacteria farmer myself, yes


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Tri's LAGM v2.022:raver2:Links I value more than my core memories:raver2:Sexy TEKs

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InvisiblemistamonstaS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri-Polar]
    #27562581 - 11/30/21 03:47 AM (1 month, 17 days ago)

Oh goody, I grew my first bacteria. I think I'll name him George :smile:


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Offlinerockyfungus
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Re: AGAR ENVY! (Anything and All things agar!) [Re: mistamonsta]
    #27562639 - 11/30/21 06:00 AM (1 month, 17 days ago)

@the sterile water

Water is an amazing solvent. It can't hurt anything if you got a good technique. Especially for those that don't follow the crowd.

Faht uses a surgical technique. Did you come across that on your own? Or watch a surgery with 2 ports to work?
Fuck it time to get graphic!


edit: it's 4 am I can't figure format. Fucking youtube. Google 2 port laparoscopic or 2 port pars plana...


Edited by rockyfungus (11/30/21 06:07 AM)


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OfflineEnDelfisE
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27562677 - 11/30/21 07:44 AM (1 month, 17 days ago)

WOW beautiful! Amazing work my friend, as always!

May I ask how can you tell when it's a monocalture ?


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OfflineGuerrilla
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Re: AGAR ENVY! (Anything and All things agar!) [Re: EnDelfisE]
    #27562684 - 11/30/21 07:53 AM (1 month, 17 days ago)

Naw that sterile water is just one big unnecessary step. You'll find that the people that say their culture "looked" clean and fucked up when it went to jars are either people that don't know what a truly clean culture looks like, or they're still a beginner and need to work on their sterile technique.

With all respect in no way should there need to be this additional step. Nobody has ever felt it necessary, and for good reason, especially those knowing what they're doing and maintaining a 0% contam rate throughout.

Quote:

EnDelfisE said:
WOW beautiful! Amazing work my friend, as always!

May I ask how can you tell when it's a monocalture ?





When there are absolutely NO sectors in the mycelium. You will never ever achieve it from transfers alone.


--------------------


Yup it’s me again. Shit, I post too much..

fahtster said:
The only bad experience is the one not had.


Edited by Guerrilla (11/30/21 07:54 AM)


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InvisibleaNewBeginning

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
    #27562784 - 11/30/21 09:59 AM (1 month, 17 days ago)

My Ps. cyan culture.

This is T7. I'm just transferring to see how the mycelium changes over time.





I have a better candidate for these tests so maybe this culture will get retired.


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InvisibleaNewBeginning

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
    #27562789 - 11/30/21 10:03 AM (1 month, 17 days ago)

Quote:

Guerrilla said:
When there are absolutely NO sectors in the mycelium. You will never ever achieve it from transfers alone.




Never as in it's not possible or never as in it's unfeasible?


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InvisiblefahtsterM
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
    #27562801 - 11/30/21 10:18 AM (1 month, 17 days ago)

Quote:

Guerrilla said:
Naw that sterile water is just one big unnecessary step. You'll find that the people that say their culture "looked" clean and fucked up when it went to jars are either people that don't know what a truly clean culture looks like, or they're still a beginner and need to work on their sterile technique.

With all respect in no way should there need to be this additional step. Nobody has ever felt it necessary, and for good reason, especially those knowing what they're doing and maintaining a 0% contam rate throughout..




Word :thumbup:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster] * 1
    #27562897 - 11/30/21 11:43 AM (1 month, 17 days ago)

Rw x twc nice uniform growth



Nats outperforming the rw x twc



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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster]
    #27562898 - 11/30/21 11:44 AM (1 month, 17 days ago)

:hereyougo:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #27563484 - 11/30/21 06:58 PM (1 month, 16 days ago)

edit: wow that was old how did I not catch that

:wooawesome:

Pic tax:
New (small but significant) additions to my agar -- thanks to y'all for the recommendations!!!


--------------------
I'm a mountain-loving guy determined to grow a mushroom. Thanks to everyone for sharing what they know :smile:.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx]
    #27563504 - 11/30/21 07:11 PM (1 month, 16 days ago)

Expensive yeast extract just seems expensive. I toss like 2g of flake and just shake a few times hot. The neon colors look nice, make some bright ice cream.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Quirkmeister92]
    #27563511 - 11/30/21 07:14 PM (1 month, 16 days ago)

I just bought the same yeast and it showed up yesterday, buying the neon colors now lol. Thanks gois


--------------------


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Quirkmeister92]
    #27563512 - 11/30/21 07:15 PM (1 month, 16 days ago)

Quote:

Quirkmeister92 said:
Expensive yeast extract just seems expensive. I toss like 2g of flake and just shake a few times hot. The neon colors look nice, make some bright ice cream.



:awetrippie: so many colors...

Didn't really do my homework as well as I usually do on the yeast. Saw a few mention it alongside clear and went for it -- flakes will be tested as well for science (and because I'll be doing this awhile and like to save $).


--------------------
I'm a mountain-loving guy determined to grow a mushroom. Thanks to everyone for sharing what they know :smile:.

Updated PF-Tek | Bods Oats | p9 Videos | Water Chamber | OG Spore Print | ZipBLOCKs | D3s Perfect Agar

Marijuana seems to help with patience (looks at jars/plates/boxes/tubs/zipblocks). (current prints: none -- working on that :wink:)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx] * 2
    #27563513 - 11/30/21 07:15 PM (1 month, 16 days ago)

Clean Germ Plate


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa] * 2
    #27563518 - 11/30/21 07:19 PM (1 month, 16 days ago)

Yeast and neon colors? I remember when any agar work was considered advanced and now i cant even keep up with the cool kids... :lmafo:


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OnlineAbabyphoenix
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #27563525 - 11/30/21 07:26 PM (1 month, 16 days ago)

Quote:

LotKid said:
Yeast and neon colors? I remember when any agar work was considered advanced and now i cant even keep up with the cool kids... :lmafo:



Im just a sucker for cute colors And D3 personally recommended the yeast. Colors really dont do shit the myc eats it anyway after so long. (not that u dont know that but. Just tellin myself really... I just like color:tongue:)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ababyphoenix] * 1
    #27563530 - 11/30/21 07:29 PM (1 month, 16 days ago)

every time I pour clear plates I think 'shit, I wish I added color!' but every time I color my plates I think 'fuck! I like clear plates better'...am I the only one? lol sorry...my wife is sick as a dog so I'm hiding from her in my office with my kegerator gettin LIT!


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