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OfflineQuirkmeister92
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Registered: 02/01/18
Posts: 8,510
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid] * 1
    #27563533 - 11/30/21 07:30 PM (1 month, 25 days ago)

I was making shitty low nute trash for a while alongside massive contam vectors. I fixed the vectors and moved to a more standard agar recipe. 10g agar, 8g malt, 2g yeast flake, 500ml distilled water. My cultures love it. It's a nice yellow/tan color and the white myc stands out distict enough I don't need more color. I could try charcoal again just to use it up.


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Team wheat...
Quirks Tub Prep


Edited by Quirkmeister92 (11/30/21 07:31 PM)


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Offlinerockyfungus
dirty
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Registered: 03/01/21
Posts: 736
Loc: Front range
Last seen: 6 hours, 30 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: Quirkmeister92] * 1
    #27563556 - 11/30/21 07:42 PM (1 month, 25 days ago)

Just getting over the flu so cult has caught up.
Clear is superior. Black looks nice though.

Been messing with master's mix/verm cake. No grain
Some gals, pan cyans, enoki, shiitake. Semp about to hit bulk (by feel hay, coffee grounds, coir)
Excited to dip into these dime bags of melted agar from over the pond. (solidified in fridge)


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LAGM 2022


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InvisibleLotKid
Never.Trust.A.Prankster
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Registered: 01/07/17
Posts: 5,780
Loc: Shakedown St.
Trusted Cultivator
Re: AGAR ENVY! (Anything and All things agar!) [Re: rockyfungus]
    #27563651 - 11/30/21 08:49 PM (1 month, 25 days ago)

I just mix agar and malt exract 50/50. 40 grams of that mix into a liter of water and then sterilzed for 30 min. Plates arent clear put not  opaque. I get strong growth and can see what i need to see. I've used coloring in the past and it does have its uses. I just dont use it much these days.


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear::dancingbear:
#ParafilmGang
 


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InvisibleWall.E
Bacteria's Bitch
Male


Registered: 06/05/20
Posts: 2,482
Loc: Fungal Void
Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid] * 1
    #27563710 - 11/30/21 09:33 PM (1 month, 25 days ago)

:whathesaid:

I usually do roughly 7.5g of both lme and agar with 500 mL water in a quart ziploc and hit it with the stick blender until it foams up to the top then pour and pc


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Life’s shit, but I’m loving it



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InvisibleScrimshaw
Sponsored by Sterilite
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Registered: 06/27/14
Posts: 559
Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #27563717 - 11/30/21 09:35 PM (1 month, 25 days ago)

Do you ever get a bad batch of plates? By bad I mean unsterile right out of the sleeve?

I ordered some cheap 100mm plates and roughly 50% had bacteria or mold within days. I chalked it up to poor technique and then poured multiple sleeves of 90mm from another batch, not a contam in sight.

Then as a test, I poured half 90mm and half 100mm from the suspicious batch in the same session. The 90s were fine and half the 100s contamed again.

I’m now confident that batch of plates were shit, and fortunately I only ordered a small amount of them, but it makes me hesitant about pulling the trigger on 500 dishes for something like that to happen again.


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Scrimshaw’s LAGM 2022


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InvisibleLotKid
Never.Trust.A.Prankster
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Registered: 01/07/17
Posts: 5,780
Loc: Shakedown St.
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Scrimshaw]
    #27563835 - 11/30/21 10:55 PM (1 month, 25 days ago)

Sleeves of improperly sterilized plates is why i switched to glass plates and sterilizing them myself.... so, yeah....


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear::dancingbear:
#ParafilmGang
 


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OfflineSpaceBabyS
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 1,570
Loc: SPACE
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #27563972 - 12/01/21 12:52 AM (1 month, 25 days ago)

Quote:

Crackatoa said:
Clean Germ Plate





:solidnod:


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK
Cult Queen:

Another Day's Work:



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OnlineEnDelfisE
Rider On The Storm
I'm a teapot User Gallery


Registered: 09/07/12
Posts: 432
Loc: Temple of Demetra
Last seen: 5 minutes, 49 seconds
Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
    #27564063 - 12/01/21 03:39 AM (1 month, 25 days ago)

Quote:

Guerrilla said:
When there are absolutely NO sectors in the mycelium. You will never ever achieve it from transfers alone.




Yes... that's what I'm coming to realize.. Even if I take the smallest piece of a sector like single hyphae.. I still see some genetic diversity in the transfer ?

But how is it possible to narrow it down to a singularity ?!
is it even necesery? what do you think ?


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Edited by EnDelfisE (12/01/21 03:41 AM)


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InvisiblemistamonstaS
Threadkiller
I'm a teapot User Gallery


Registered: 07/06/11
Posts: 868
Loc: Uranus
Re: AGAR ENVY! (Anything and All things agar!) [Re: EnDelfisE]
    #27564135 - 12/01/21 06:19 AM (1 month, 24 days ago)

I thought cloning from a selected fruit was the only way to get a monoculture...

Or maybe when starting with monokaryotic cultures from single spores for cross breeding purposes?


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MistaMonsta's LAGM 2022


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Onlineschpat
Newb
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Registered: 09/26/21
Posts: 182
Loc: South Africa
Last seen: 13 seconds
Re: AGAR ENVY! (Anything and All things agar!) [Re: mistamonsta]
    #27564171 - 12/01/21 07:17 AM (1 month, 24 days ago)

All this talk of nutritional yeast extract has peaked a question in my mind.  How is myc with salt?  The reason I ask is has anyone ever tried marmite as a yeast extract?  Its concentrated yeast extract with salt added that you spread on toast.  And 100% water-soluble.

https://world.openfoodfacts.org/product/60050168/marmite-yeast-extract


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schpat's LAGM 2.022 Grow Log


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OfflineDERRAYLD
Constructus
Male

Registered: 05/13/02
Posts: 3,817
Loc: South Africa
Last seen: 31 minutes, 37 seconds
Re: AGAR ENVY! (Anything and All things agar!) [Re: schpat] * 1
    #27564366 - 12/01/21 10:47 AM (1 month, 24 days ago)

This RW x TWC plate has me all excited and tingly in my nether regions....



Grow Nats grow



Mvp set to be transferred


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InvisibleMysticMycologist
Sponge-like
Male


Registered: 10/14/21
Posts: 823
Loc: seeking samadhi
Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27564386 - 12/01/21 11:03 AM (1 month, 24 days ago)

Looking good. I can’t wait to get my hands on some nats.
:vibin:



Some PE T1s finally showing some organization. Check out that little tomentose sector at 2o’clock. I’m finally getting some vigorous growth. Hoping to go to rye after another transfer or two.



Some other T1s from the same germ plate.




--------------------
Two eyes to look, One eye to see.

Prying open my third eye

:sporedrop:
I’m looking for a good Psilocybe ovoideocystidiata print for trade or purchase. If you have one, shoot me a DM.


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Invisiblebigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 1,966
Loc: Gamehenge
Re: AGAR ENVY! (Anything and All things agar!) [Re: MysticMycologist]
    #27564638 - 12/01/21 01:49 PM (1 month, 24 days ago)

PE T2 that I should have sent to grains

PE T3 that will soon go to grains

T4 that will soon go to grains


For some reason this cult season I convinced myself that I should take transfers out to T4+ instead of my usual T2-3 which I'm realizing is stupid lol


--------------------
Bigfoots LAGM v. 2.022


At this point 'conspiracy theories' should just be called spoilers


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OfflineA.k.a
Stranger
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Registered: 10/27/19
Posts: 14,339
Loc: Gaming the system
Last seen: 7 hours, 10 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: EnDelfisE]
    #27564729 - 12/01/21 03:08 PM (1 month, 24 days ago)

Quote:

EnDelfisE said:
Quote:

Guerrilla said:
When there are absolutely NO sectors in the mycelium. You will never ever achieve it from transfers alone.




Yes... that's what I'm coming to realize.. Even if I take the smallest piece of a sector like single hyphae.. I still see some genetic diversity in the transfer ?

But how is it possible to narrow it down to a singularity ?!
is it even necesery? what do you think ?






People will dilute spores and then use a microscope and a needle to scoop up a single spore and put it on a plate. Then it’ll grows but won’t fruit unless you introduce it to a second single spore colony and I think that’s the only way to do it.


But also there’s no point really unless you’re going to put tons of work into it. Narrowing isn’t always a good thing.


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LAGM2020


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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,318
Loc: Year Zero
Trusted Cultivator
Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a] * 1
    #27564817 - 12/01/21 04:20 PM (1 month, 24 days ago)

You can di / mon pair as well. They should still take to eachother or have significant chance



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OfflineQuirkmeister92
Street Doctor
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Registered: 02/01/18
Posts: 8,510
Loc: Grateful Dead USA
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc] * 1
    #27564879 - 12/01/21 05:23 PM (1 month, 24 days ago)

I would go really wide and then select clones before going narrow.

I tried dragging a caps gills against a few plates today instead of swabbing.:shrug:


--------------------
Team wheat...
Quirks Tub Prep


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InvisibleaNewBeginning

Registered: 06/26/21
Posts: 708
Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning]
    #27565437 - 12/02/21 12:24 AM (1 month, 24 days ago)

Quote:

aNewBeginning said:
Could this be the start of plate pins?











Looks like a few pins are forming


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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,318
Loc: Year Zero
Trusted Cultivator
Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning] * 3
    #27565445 - 12/02/21 12:30 AM (1 month, 24 days ago)

You could almost say its a …. New beginning


I’ll see myself out…🤣


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InvisibleaNewBeginning

Registered: 06/26/21
Posts: 708
Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27565448 - 12/02/21 12:33 AM (1 month, 24 days ago)

Quote:

D3_Myc said:
You could almost say its a …. New beginning


I’ll see myself out…🤣




That's actually quite fitting with what I'm trying to achieve with mush cult. I like that :smile:


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OfflineAbabyphoenix
You
Male User Gallery


Registered: 03/21/19
Posts: 821
Loc: Human Body
Last seen: 2 hours, 29 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc] * 1
    #27565455 - 12/02/21 12:41 AM (1 month, 24 days ago)

Quote:

D3_Myc said:
You could almost say its a …. New beginning


I’ll see myself out…🤣



:nogodno:


--------------------


:mushroom2::sporedrop:Pho's Mushi Aventures:sporedrop::mushroom2:
:peace: LAGM 2022 :peace:


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