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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27540379 -

I pour very quick and it works out, but I thought about getting this before:
https://www.amazon.com/IVYX-Scientific-Digital-Laboratory-RT-100%C2%B0C/dp/B07T81P5KN

I think Bod uses something like this, and has a recommended temp for what he uses, wouldn't be hard to find.


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--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27540415 -

"I have about 8 or so blank (blank? empty? non-inoculated?)"

Virgin
:superthrust::splooge:

I don't bother with switching up agar media or warm water baths.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: verum subsequentis] * 1
    #27540421 -

Ol' Wall'a Plates we call him. Huge wall. Tremendous.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27540453 -

Plates, plates, plates! (I need practice taking pictures of the damn things. I need to make a photo box for plates or something. ANYWAYS.)

These are pretty much all tissue transfers done in a SAB with either flamed tweezers or a scalpel. MEA (probably on the higher nutes side).

I'd love for some of the "BM Chonger" plates to make it -- alongside the MDKs and the GTs of course :wink:.

Here are a few (there are more) that I'm considering transferring sooner than later! Any and all advice is *welcome* and appreciated -- this is my first time with anything except bacteria on agar.



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Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx]
    #27540525 -

How long does everyone let their plates sit before using/wrapping/storing them? I usually go 24 hours but I need my office soon and I've got my SAB set up right in the middle of my desk lol. Didn't think that one through...


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I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420]
    #27540534 -

I been using my slow cooker for agar bottles. Keeps em gelled as long as you need.

Just ordered the gellan gum I mentioned previously, I’ll be testing and posting results.

Also ordered potato flakes, dex and charcoal to test my experience with those. Not particularly interested in any of those other than the gellan but it gives me something to do.


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Being pissed on does not make you a real man.

...OR DOES IT?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27540562 -

bigfootscreepyuncl said:
How long does everyone let their plates sit before using/wrapping/storing them? I usually go 24 hours but I need my office soon and I've got my SAB set up right in the middle of my desk lol. Didn't think that one through...


As soon as they've solidified. Depends on the temp they were poured, how high they are stacked (middle plates take the longest) and ambient room temp. I'd say an hour or so should easily do. If in a hurry and curious, just carefully extract the middle most plate from the biggest stack and see if she's flaccid or aroused.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx] * 1
    #27540607 -

xVadisx said:
Plates, plates, plates! (I need practice taking pictures of the damn things. I need to make a photo box for plates or something. ANYWAYS.)

These are pretty much all tissue transfers done in a SAB with either flamed tweezers or a scalpel. MEA (probably on the higher nutes side).

I'd love for some of the "BM Chonger" plates to make it -- alongside the MDKs and the GTs of course :wink:.

Here are a few (there are more) that I'm considering transferring sooner than later! Any and all advice is *welcome* and appreciated -- this is my first time with anything except bacteria on agar.





That's a lot of tissue samples. Take it from ol wall o plates himself, it is possible to bite off more than one can chew and end up accomplishing less than you would have if you had kept it a little simpler. You do you though. OK........ here we go

Pic one: Looks like it may be clean or may be fighting something. I see no particular place that looks better than any other. Just transfer and see what happens.

Pic two: Looks to be certainly fighting something. I'd just let her keep growing and see if anything good looking appears.

Pic three: Not the best pic but I'd choose the lower plate and leave the upper to chill and grow out. Bottom plate looks hopeful. I'd transfer from 11:30 - 11:45.

Pic four:
Top: Looks hopeful. I'd probably snatch up the 8 o'clock portion.
Second row:
    Left culture: Probably be OK taking from around 2  o'clock or 6 o'clock on the right growth.
    Right culture: Meh. Seems quite fishy. Might get something out of the 1 or 4 positions. PS, are you cloning from gill tissue?
Third row:
    Left culture: Looks pretty good. I'd snatch at 8 o'clock.
    Right culture: Meh. Looks like trash.

Pic five:
      Top left and bottom right cultures look most hopeful. The pics are out of focus so it's really hard to say too much. I think I'd snatch 9 o'clock from the top left culture and 5 o'clock from the bottom right culture. PS, confirmed cloning from gill tissue. This results in the germination of shit tons of spores and negates the main points of cloning.

Pic 6: Bad pic. I can't tell.

Pic 7: 4 - 6 o'clock look pretty hopeful.

Pic 8: I'd let her grow and show her true colors for a couple days

Pic 9: Looks pretty hopeful. Still a bit early to tell but you could probably snatch up something useful from almost anywhere. I think I'd let her grow as well.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) *DELETED* [Re: verum subsequentis]
    #27540621 -

Post deleted by Failboat

Reason for deletion: S


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Never give up, never surrender.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat]
    #27540630 -

Thank you!

Dually noted about the gill tissue. I actually suspected as much (and had already taken some tissue from the stipes). I’ve also accepted that I’ve taken on a bit much. Simplifying things seems good. Transfers shall be taken. Updates in a few.

(edit) update: In efforts to simplify, I took the best and removed the gill tissue plates and any showing signs of ick to the we'll see later stack. I'm going to let these go until at least tomorrow morning before making any transfers.


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Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

Edited by xVadisx (11/12/21 03:10 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx]
    #27540754 -

Just finished up about an hour in the SAB taking nice transfers and wrapping my newly poured plates. As I'm packing up I held a plate up to the light to see how clear I got em and EVERY. SINGLE. FUCKING. PLATE has 1/16"-1/8" speckles all over it..! Are there even any contaminants that would germinate in 15 hours? I've only ever had like 3 plates contam out of ~500 plates. WTF!

All the speckles look the same and a large majority of them seem to be surrounding an air bubble/divot/imperfection on the plate so I'm really hoping it's the result of pouring agar that is very close to gel and that I didn't contaminate 2 sleeves and waste all my t-2s.


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I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl] * 2
    #27540761 -

Excited to finally have a few ape cultures to run



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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27540762 -

It's almost certainly not a living thing but I'd need to see good clear pics.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: verum subsequentis]
    #27540766 -

verum subsequentis said:
It's almost certainly not a living thing but I'd need to see good clear pics.


These are the best I could get - it's a pretty tricky picture to take because they only show up when the light hits them head on but then my phone blocks the light and they disappear. The speckles look like hard water stains on glass, but obviously aren't lol.



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I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27540772 -

Those will do. Tis my belief that you are seeing minute variances in the agar surface that are catching light in a different way than the flat portions. I've seen similar from time to time. Dead give away is that it's only happening around the bubbles. Makes sense that the bubbles would cause curvature of the surface. Make sense?


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27540774 -

D3_Myc said:
Excited to finally have a few ape cultures to run




Those are the nicest looking apes I've ever seen, that last one looks like feathers it's so perfect...your plates never disappoint!


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I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: verum subsequentis]
    #27540777 -

verum subsequentis said:
Those will do. Tis my belief that you are seeing minute variances in the agar surface that are catching light in a different way than the flat portions. I've seen similar from time to time. Dead give away is that it's only happening around the bubbles. Makes sense that the bubbles would cause curvature of the surface. Make sense?


Perfect sense, thanks for reassuring me! That's what I figured it was but I've been out of practice for a while and had one of those 'oh shit' moments lol


--------------------



I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27540794 -

D3_Myc said:
Excited to finally have a few ape cultures to run




Just ordered me some charcoal. 1g to 500ml?


--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla] * 1
    #27540803 -

I do a half spoonful. My best estimate is 1g per 100ml but adjust accordingly.

I went from having no ape cultures to 7 different varieties thanks to amazing friends. Excited to do some comparison grows. The black #1 is one I’m excited to potentially use in some crosses.


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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27540809 -

D3_Myc said:
I do a half spoonful. My best estimate is 1g per 100ml but adjust accordingly.

I went from having no ape cultures to 7 different varieties thanks to amazing friends. Excited to do some comparison grows. The black #1 is one I’m excited to potentially use in some crosses.


Cheers D3. Half a US tablespoon is 7.39g so I’ll try different amounts around this.


--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   North Spore Cultivation Supplies   Myyco.com APE Liquid Culture For Sale   Mushroom-Hut Substrate Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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