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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tumerz]

1
#27206353 - 02/14/21 09:16 PM (5 years, 7 months ago)

top plate, bottom wedge anywhere from 1:30 -3:30 and or around 6

Mid plate bottom wedge 6 oclock

Bottom plate maybe around 7. I’d go for smaller xfers if you can manage. Def don’t cut into any of that raised up myc, can’t tell if it’s crawling over something or just environmental but I wouldn’t trust it.

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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Tumerz

Registered: 06/24/20
Posts: 123
Last seen: 15 days, 2 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]

1
#27206376 - 02/14/21 09:35 PM (5 years, 7 months ago)

Sweet, thanks for the advice. I just poured some plates so hoping to take some transfers tomorrow if I have time.

I hear you on the smaller wedges! I'll definitely be doing that. The large ones I've been doing have been hard to see individual growths. I took some cube transfers with some .5cm x .5cm ones and it's much easier to see the individual sections.

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clockworkshroom
Stranger

Registered: 02/20/20
Posts: 689
Last seen: 8 months, 8 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tumerz]
#27207028 - 02/15/21 08:56 AM (5 years, 7 months ago)

Upgraded my hole punch to this ghetto version of d3monic's tool. Bent metal straw with a tape handle. I'll test it out later.

I made up some plates with activated charcoal powder and 1g yeast and got sediment dried to the agar bottle after pc-ing despite using a hand blender to mix it thoroughly before hand. Not sure if it was the yeast (used Braggs brand, same as some other forum uses) or charcoal powder. No issue really, it still made jet black plates but may not have the yeast in and the whole reason I made them with yeast was to test with if it prompted rhizo.

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p9hu7's clean spawn thread

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DontMatter
Stranger

Registered: 04/11/20
Posts: 18
Last seen: 5 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: clockworkshroom]
#27207098 - 02/15/21 09:41 AM (5 years, 7 months ago)

Hey guys,
all my last grows went green before first flush so i decided to get into agar...

i cloned two pins (GT) and would like some opinions

1. Cluster pin
original plate:

T3 plate 1:

T3 plate 2:

2. Fat single pin
original plate:

T3 plate 1:

T3 plate 2:

would you spawn any of these?
since i do have only small pins on the original plates, will all my mushrooms be only small pins and not mature if i spawn them?

Edited by DontMatter (02/15/21 09:54 AM)

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Gastronomicus
3-0-G

Registered: 03/31/05
Posts: 9,746
Last seen: 2 months, 11 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DontMatter]
#27207194 - 02/15/21 10:35 AM (5 years, 7 months ago)

The only one I wouldn't run is the second T3 plate 1. That wedge of clear stuff at 12 o'clock looks sketchy, I'd transfer from the bottom of that one.

The others look good to go

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Make my Funk the P Funk, I wants to get Funked up

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DontMatter
Stranger

Registered: 04/11/20
Posts: 18
Last seen: 5 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Gastronomicus]
#27207234 - 02/15/21 11:03 AM (5 years, 7 months ago)

Gastronomicus said:

The only one I wouldn't run is the second T3 plate 1. That wedge of clear stuff at 12 o'clock looks sketchy, I'd transfer from the bottom of that one.

The others look good to go

thats why i took only one picture of that one :laugh:

:laugh:

prepping some rye now...

how likely will they mature and not stay pins?

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Gastronomicus
3-0-G

Registered: 03/31/05
Posts: 9,746
Last seen: 2 months, 11 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DontMatter]
#27207240 - 02/15/21 11:05 AM (5 years, 7 months ago)

I'm not sure I understand the question. You mean on the plates? Typically they will grow into a mature, albeit tiny mushroom. People usually just snag those to clone with, I wouldn't put them to grain or anything. Just the nice transfers that you have

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Make my Funk the P Funk, I wants to get Funked up

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DontMatter
Stranger

Registered: 04/11/20
Posts: 18
Last seen: 5 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Gastronomicus]
#27207256 - 02/15/21 11:20 AM (5 years, 7 months ago)

i meant because on the original plate the pins just stay small, if i spawn the good looking plates, may i have cloned "small fruit genetics" and i will only get tiny fatties in my tubs?

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Gastronomicus
3-0-G

Registered: 03/31/05
Posts: 9,746
Last seen: 2 months, 11 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DontMatter]
#27207268 - 02/15/21 11:37 AM (5 years, 7 months ago)

Nah, the size difference is because there's not a lot of nutrients on the small agar puck when compared to a full grain jar. Some people have cloned plate pins and wound up with small fruits but that's either bad luck or a different factor.

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Make my Funk the P Funk, I wants to get Funked up

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DontMatter
Stranger

Registered: 04/11/20
Posts: 18
Last seen: 5 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Gastronomicus]
#27207276 - 02/15/21 11:44 AM (5 years, 7 months ago)

well, i'll grow them out and see what happens :grin:

:grin:

TY

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gt40
I will proof smthng

Registered: 09/17/12
Posts: 505
Last seen: 4 months, 6 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DontMatter]
#27208859 - 02/16/21 06:00 AM (5 years, 7 months ago)

guys, is it looks like normal rhizo? had some issues with trich in monos on final stage and have suspicious about initial material

that how it looks on coir bulk now

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Ecuador on bloody coir
Panaeolus Cyanescence Jamaica

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savan73
Stranger

Registered: 05/05/20
Posts: 254

Re: AGAR ENVY! (Anything and All things agar!) *DELETED*

[Re: gt40]
#27208955 - 02/16/21 07:53 AM (5 years, 7 months ago)

Post deleted by savan73

Reason for deletion: .

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Ababyphoenix
You

Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 8 days, 18 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: savan73]

1
#27209116 - 02/16/21 09:59 AM (5 years, 7 months ago)

One of my many fat dish pins from @roarkell's melmac culture. Decided id snag this one and shes beautiful

:ohwow:

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:sporedrop:

:aum:

Pho's MushiAventures: :aum:

:aum:

:sporedrop:

:heartpump:

:heartpump:

:peace:

:peace:

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Ababyphoenix]
#27209178 - 02/16/21 10:59 AM (5 years, 7 months ago)

I love the thick rhizo growth you get with tissue clones. Makes you tempted to just send it. I tried that recently, even though it looked nice it triched out in the jar unfortunately.

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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Lenz
Misunderestimated

Registered: 08/17/20
Posts: 692
Loc: Fungistan

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27209204 - 02/16/21 11:13 AM (5 years, 7 months ago)

This kind of raised growth indicates bacteria right? Should I try and transfer away, hot pour, or BRF puck it?

As opposed to a nice flat clone plate:

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:chems:

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roarkell
The Roar Master!

Registered: 08/29/19
Posts: 2,223
Loc: Southern WI

Last seen: 2 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lenz]
#27209261 - 02/16/21 11:44 AM (5 years, 7 months ago)

Lenz said:

This kind of raised growth indicates bacteria right? Should I try and transfer away, hot pour, or BRF puck it?

As opposed to a nice flat clone plate:

Idk about it indicating bacteria... I get plates like that fairly often and I haven't noticed a major issues.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: roarkell]
#27209808 - 02/16/21 04:58 PM (5 years, 7 months ago)

Trying out this chef master brands purple, see how it performs. I did one drop purple and one drop blue. The purple has red 3, red 40 and one the blues already in it. I’m hoping that there’s so little red 3 that it doesn’t negatively effect any plates. I’ll be sure to do side by sides with it to compare growth. Only pouring a 10 stack with it incase it sucks

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

Edited by D3_Myc (02/16/21 05:08 PM)

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Phony Phone

Registered: 09/19/20
Posts: 666

Last seen: 1 year, 18 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27209861 - 02/16/21 05:15 PM (5 years, 7 months ago)

Lenz said:

This kind of raised growth indicates bacteria right? Should I try and transfer away, hot pour, or BRF puck it?

As opposed to a nice flat clone plate:

I'd really like to know the answer to this too. I used a culture with raised fluffs like this and there was obvious bacteria in the jar, spikey stressed mycelium and a few wet spots, also creamy white as opposed to the healthy transparent growth I get from healthy jars.

My experience so far tells me this MIGHT be bacterial sample but my experience doesn't mean anything because I havent confirmed over and over.

D3monic said:

Trying out this chef master brands purple, see how it performs. I did one drop purple and one drop blue. The purple has red 3, red 40 and one the blues already in it. I’m hoping that there’s so little red 3 that it doesn’t negatively effect any plates. I’ll be sure to do side by sides with it to compare growth. Only pouring a 10 stack with it incase it sucks

Those colors are just beautiful...... :ohwow:

:ohwow:

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My Journal, where I gather useful stuff over time

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,222
Loc: Gaming the system
Last seen: 8 hours, 40 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Phony Phone]
#27209877 - 02/16/21 05:24 PM (5 years, 7 months ago)

I don’t think it’s bacteria or anything, sometimes it just does that.

Don’t see it much with cubes, but natalensis...

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RamJam
Stranger

Registered: 10/21/20
Posts: 337
Last seen: 5 years, 15 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27210385 - 02/17/21 02:20 AM (5 years, 7 months ago)

I'm having a hell of a time getting anything from these prints I got. Anyone see anything I could salvage from any of these? I was thinking maybe this tek https://www.shroomery.org/forums/showflat.php/Number/24806569#24806569
But it just looks like 100% contamination to me...

I have no contam issues like this in my other agar work, def dirty prints. And only did small drops with an inoculation loop in the center of each Y slots. (I have since gotten some huge dishes and used gentamicin and the following tek with the multiple swipes with fired loop in between swipes https://www.shroomery.org/forums/showflat.php/Number/24330957/fpart/all/vc/1)

Also, can't get anything at all to happen with the 2 PE swabs I got. If anyone has PE to trade PM plzzz

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