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Ababyphoenix
You

Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 2 months, 22 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nobler Hino]
#27100933 - 12/21/20 01:34 PM (5 years, 8 months ago)

all going to grain soon :smile:

:smile:

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:sporedrop:

:aum:

Pho's MushiAventures: :aum:

:aum:

:sporedrop:

:heartpump:

:heartpump:

:peace:

:peace:

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Eugene Gesuale
Jar-Sniffer

Registered: 04/12/20
Posts: 1,920
Loc: The Basement

Last seen: 3 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Ababyphoenix]
#27100993 - 12/21/20 02:13 PM (5 years, 8 months ago)

What do you guys think of this. I can’t tell if it’s bacteria or not because the only bright white myc I see is coming out the top of the spore clumps

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Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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verytastycheese
Tripmaster

Registered: 11/11/14
Posts: 101
Last seen: 5 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#27101004 - 12/21/20 02:21 PM (5 years, 8 months ago)

Inthepit said:

verytastycheese said:

I took 4 plates from my original spore streak so labeled them A1-A4 - all were very separate clusters of spores, and started T1++ from there. But since I take more than one transfer off each plate I had to start T3A,B,C etc. The system is starting to get long and disorganized. I'm thinking of just numbering them and keeping a spreadsheet of the lineage instead. So yeah Amazonian, Third agar plate, 4th transfer, 4th wedge of that transfer.

My "system" has been getting more complex also, but what good is it?
Ha, it seems like where a winner, RR picks a clone, came from doesn't matter.
Only that you have the winner as a Master. The date, number of transfers is
mildly interesting, but it's what looks worth sending to grain, right?

All those old plates don't mean diddly, am I right?
I'm keeping old plates around in case one pins, but even a pin isn't that big of a deal.
And by the time one pins yer prolly already going for spawning.
like this by Ima Trooper
None of these plates were worth anything after I went to grain...

The only value is in a clone, that
has most of the characteristics yer lookin for.

Nice notes! I might have to copy some of your spreadsheet style.

I'm mostly keeping track to figure out where I've gone wrong (In the past) and trying to tighten up the whole process. But does it not make sense to keep your old culture plates that haven't been stretched too far, in case it was a bomb culture and you want to store it long term, or want to start another batch without using spent/recessive genetics? Also to compare my future work to in case I've forgotten what I'm really looking for.

I've been on a losing streak lately and I figure more detailed notes will clear it up.

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Yeetusdeetus

Registered: 11/23/19
Posts: 1,242
Last seen: 1 month, 28 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: verytastycheese]
#27101404 - 12/21/20 06:37 PM (5 years, 8 months ago)

Do these look funky or is it me?

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verytastycheese
Tripmaster

Registered: 11/11/14
Posts: 101
Last seen: 5 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Yeetusdeetus]
#27101426 - 12/21/20 06:49 PM (5 years, 8 months ago)

Yeetusdeetus said:

Do these look funky or is it me?

My newfound paranoia for anything but silky rhizo growth has me saying nope to those.

But also how'd you get your agar so clear??

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Yeetusdeetus

Registered: 11/23/19
Posts: 1,242
Last seen: 1 month, 28 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: verytastycheese]
#27101485 - 12/21/20 07:13 PM (5 years, 8 months ago)

400ml water
6g agar
5g malt

Shake, boil in microwave, shake, then pc :gangsta:

:gangsta:

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verytastycheese
Tripmaster

Registered: 11/11/14
Posts: 101
Last seen: 5 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Yeetusdeetus]
#27101510 - 12/21/20 07:22 PM (5 years, 8 months ago)

Hmm I wonder if I'm using too much agar/everything then.
500ml water
10g agar
7g LME (previously 10)

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Yeetusdeetus

Registered: 11/23/19
Posts: 1,242
Last seen: 1 month, 28 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: verytastycheese]
#27101539 - 12/21/20 07:34 PM (5 years, 8 months ago)

Yeah that sounds like a lot :lol:

:lol:

. Use what works for you but if you’re not getting desirable results that might be why

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,216
Loc: Gaming the system
Last seen: 20 hours, 22 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Yeetusdeetus]

1
#27101547 - 12/21/20 07:40 PM (5 years, 8 months ago)

Those plates look fine to me

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jcm4620
Stranger

Registered: 05/26/19
Posts: 6,700
Last seen: 4 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Yeetusdeetus]

2
#27101554 - 12/21/20 07:42 PM (5 years, 8 months ago)

well i guess this place is as good as any to post this of some transfers from jakes nec-d pan these were all taken only a day n a hlf ago off a barely germinating streak plate. i just cant get over how fast these things are moving. plus they look good

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PANAEOLUS FRUITING MADE SIMPLE

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,216
Loc: Gaming the system
Last seen: 20 hours, 22 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: jcm4620]
#27101567 - 12/21/20 07:45 PM (5 years, 8 months ago)

Man I just got a print but now I’m waiting on my case of plates before I can get it started.

I’ve had a couple take off like that but it’s crazy they all did.

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Josex
#cheat_code

Registered: 11/13/15
Posts: 8,999
Loc:

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27101575 - 12/21/20 07:46 PM (5 years, 8 months ago)

A.k.a said:

Those plates look fine to me

To me too.

Also, 2% agar and 2% LME is the gold standard, that means 10 grams agar and 10 grams LME to 500ml of water. It's fine to go lower than that but 2% nutes is not "a lot" for agar and I personally prefer it over lower nute strengths.

--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

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Yeetusdeetus

Registered: 11/23/19
Posts: 1,242
Last seen: 1 month, 28 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27101579 - 12/21/20 07:48 PM (5 years, 8 months ago)

Okay cool. Whenever I don’t get bright white myc I immediately think mold

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Josex
#cheat_code

Registered: 11/13/15
Posts: 8,999
Loc:

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Yeetusdeetus]
#27101609 - 12/21/20 08:10 PM (5 years, 8 months ago)

Yeetusdeetus said:

400ml water
6g agar
5g malt

Shake, boil in microwave, shake, then pc

Maybe you'll get thicker growth if you add 8 grams of malt to those 400ml of water.

--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

Extras:

Yeetusdeetus

Registered: 11/23/19
Posts: 1,242
Last seen: 1 month, 28 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Josex]
#27101636 - 12/21/20 08:25 PM (5 years, 8 months ago)

I’ll make a couple bottles to try next time I make agar lol. I’ve had bright white growth with this recipe before, but this is two transfers out from an iffy pinned plate I made in June. That’s why I was wondering what people with more experienced eyes thought

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Mr. Mushie

Registered: 01/16/20
Posts: 667

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Yeetusdeetus]
#27101858 - 12/21/20 11:34 PM (5 years, 8 months ago)

PESH Clone T1

Where would you transfer from on this plate?

I could grab an individual sector but wouldn't that further isolate the genetics to some extent?

There was a thread on this a few weeks back and the general consensus was that it's not going to drastically affect the genetic outcome unless many transfers were made in an attempt to further isolate.

Any thoughts Agar gurus?

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dingdonger22
Stranger

Registered: 12/14/20
Posts: 35
Last seen: 5 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Josex]
#27101864 - 12/21/20 11:41 PM (5 years, 8 months ago)

These were done with slightly less (1.5% agar/LME) as an experiment but the next ones i'll do at 2%. Is that considered to be a general sweet spot for agar / LME?

I know it is early but Im wondering if I should be looking for any characteristics that would let me know if any of these could be good candidates to go to grain or if they need more transfers. Want to make sure I'm not rushing anything or being overly impatient but also don't know exactly what would qualify something to be ready.

These are from a pin that grew on an agar plate

This is a second transfer from an APE pin on an agar plate

this is a second transfer from a MS streak plate

I can't tell if the growth on the first two (or any really) are going well enough to go to the next step but these are what I think are the best ones!

edit :: looking at this I realized I took the original sample in the transfer. how could that potentially impact things?

Edited by dingdonger22 (12/21/20 11:45 PM)

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capntrips7
Moldsmith

Registered: 10/21/20
Posts: 91
Loc: Urithiru

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]

1
#27101890 - 12/22/20 12:38 AM (5 years, 8 months ago)

I bought a cheap light box. Pics are looking way better:

There is still a bit of glare on the edges of the plates and the sides of the jars, but it isn't awful.

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: jcm4620]
#27101948 - 12/22/20 02:55 AM (5 years, 8 months ago)

jcm4620 said:

Hey JCM, I like this but, are you really taking 7 or 8 tiny transfers to a plate?

I mean, that's the first time I've seen this and am wondering
how they can grow out to pick from there, it looks so crowded.

The plate looks smaller too, 60mm?

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:sporedrop:

:sporedrop:

:sporedrop:

:sporedrop:

GETTING STARTED :sporedrop:

:sporedrop:

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,216
Loc: Gaming the system
Last seen: 20 hours, 22 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#27102010 - 12/22/20 05:08 AM (5 years, 8 months ago)

Yeah cuz with pans the type of myc is important so with all those wedges there’ll be plenty to pick from. In this case the pretty much all look good but that doesn’t usually happen. They won’t get grown out much I imagine he’s probably taking the next wedges today if it’s not already done.

Lol it does look crazy with so many though.

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