Ovoidhunter said:

These are the 3rd transfers from a old crispy peice of dried up agar that im cleaning up.

Question is how do they look to you guys? How many more transfers would you do? (I plan to do 2 more for good measure.)

Also would you take your tranfers from the first pic or the other 2?

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Looks good from here, the number of transfers is not a real measure. You can establish a clean culture from spore in 1 transfer or 30 if your technique is off.

take from all 3 plates and see whatcha get, I would almost consider dropping that 1st plate to grain tho *(assuming that more opaque area to the left of the culture looks like what you can see on the right)
Can you get pics from the side with a top view of the culture?

Edmunter said:

I reccon everyone ive not commented on is ready for masters.  Any input here would be much appreciated....

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They all look pretty spot on

TheDoobsker said:

Still working on getting something I can put to grain
These were a Z-strain clone and their first transfer
Going to transfer from these first 2 plates today. Any tips appreciated
I have yet to have any luck getting to a clean culture when transferring from plates that look like these ones

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I usually let mine grow out more before taking a transfer...but that's just me

Tormato said:

Don't remove the contam...transfer myc away to another dish

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One of Us said:

But I'll also add that transferring away from bacteria colonies like most of your pics is no big deal at all. Just take some clean myc to a clean plate.

Much more care must be taken when taking a transfer from a plate with sporulating mold, as seen in your third pic.  It can be done, you just have to use VERY slow movements to avoid getting spores all over the culture, if they haven't gotten there already. And then follow up with quick subsequent transfers as soon as you can take them, so you don't give the spores time to germinate.

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