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Re: AGAR ENVY! (Anything and All things agar!) [Re: seand04]
    #26963009 -

I have been using a 2% turbindo sugar 10% MEA agar 10% telephone agar and 5% BRF. Distilled water. Agar to LC to wbs will post pictures soon. More PE

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Re: AGAR ENVY! (Anything and All things agar!) [Re: seand04]
    #26963013 -

i got it by mistake so why not use it see how it does.  Ingredients say malted barley flour, dextrose, wheat flour.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario]
    #26963014 -

It looks like most of y'all are using petri dishes. I'm all set to do Pasty's agar TEK with the Glad Mini Rounds already, so that's what I will start with, but what is everyone's opinion on using petri dishes vs the little tupperwares? What are the benefits for doing it all legit sciency?


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"...do you think God made the world to amuse himself because he was bored? Because if so he would have to be mean." - Jack Kerouac

                             

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MycoTricho]
    #26963017 -


Here are my mea plates.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MycoTricho] * 1
    #26963020 -

MycoTricho said:
It looks like most of y'all are using petri dishes. I'm all set to do Pasty's agar TEK with the Glad Mini Rounds already, so that's what I will start with, but what is everyone's opinion on using petri dishes vs the little tupperwares? What are the benefits for doing it all legit sciency?



I use pasty plates exclusively bro. It is how good your own personal sterile technique is that defines you

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Normlizer420]
    #26963023 -

Normlizer420 said:

Here are my mea plates.



Looking good ready for the second transfer

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Normlizer420] * 1
    #26963032 -

never tried the tubberware containers.  But before i got into agar i did use the tiny ball jars with one of the brf agar teks and had good results.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: seand04]
    #26963035 -

seand04 said:
MycoTricho said:
It looks like most of y'all are using petri dishes. I'm all set to do Pasty's agar TEK with the Glad Mini Rounds already, so that's what I will start with, but what is everyone's opinion on using petri dishes vs the little tupperwares? What are the benefits for doing it all legit sciency?



I use pasty plates exclusively bro. It is how good your own personal sterile technique is that defines you



The only thing I could think of, besides having a better view, would be the fact that they are more compact. That seems like it would make it a little easier to work with.


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"...do you think God made the world to amuse himself because he was bored? Because if so he would have to be mean." - Jack Kerouac

                             

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Normlizer420] * 1
    #26963038 -

I don't know how to send links, but look up pasty plates. Pastywhyte is the person who got me into agar. So easy so super props to him, juraó:rockon:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: seand04]
    #26963040 -

I confess oops... wrong thread. I've yet to use pasty's plates. I think that I would have less contams if I did but I started with petris and am getting comfortable with them. I will use some in the future but for now, I've got a fairly high contam rate and I'm not gonna abandon my petri technique until I figure out what I'm doing wrong. I've got a thing where I hate leaving a job half finished and if I start a different tek, it'll bother me the whole time knowing that I didn't figure out my sterile petri technique.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: seand04]
    #26963041 -

seand04 said:
Normlizer420 said:

Here are my mea plates.



Looking good ready for the second transfer



Thank you :thumbup:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Normlizer420] * 2
    #26963053 -

Always,  I love this community,  going through a Divorce and this community really helps me so thank you all blessings be upon you and yours

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Normlizer420]
    #26963054 -



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"...do you think God made the world to amuse himself because he was bored? Because if so he would have to be mean." - Jack Kerouac

                             

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MycoTricho] * 1
    #26963056 -

MycoTricho said:
Pastywhyte's Easy Agar Tek



That is what I am talking about

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Re: AGAR ENVY! (Anything and All things agar!) [Re: seand04]
    #26963069 -

I bought a bunch to try but went back to petris as I preferred the visibility of petris and less to no condensation. I occasionally pour a few deli containers though for swab dunks

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario]
    #26963126 -

Doctor Mario said:
The Dalcassian said:
Anyone used low acyl Gellan gum before.
Just came across this pic and damn is it a sexy plate.





Is that plate empty or is it just that clear? That does look pretty sweet.



Sorry dude, different timezones.
It's just that clear. Stunning. It's $20 per 100g, I've ordered some to have a play


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Here Lies This Individual




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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26963128 -




Clones from a Z-strain shoebox 3rd flush

I think I chose the wrong spot on that bottom transfer 1st plate. Should have done 9-11 o'clock what do you think?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #26963133 -

I am not an expert but looks good to me

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #26963134 -

TheDoobsker said:



Clones from a Z-strain shoebox 3rd flush

I think I chose the wrong spot on that bottom transfer 1st plate. Should have done 9-11 o'clock what do you think?



you can still do it

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Re: AGAR ENVY! (Anything and All things agar!) [Re: seand04]
    #26963201 -

I’ve never tried pasty plates or any of the little Tupperware methods but everytime I go to knock up jars I wish I could just dice up and squeeze the whole puck out at once like you can with those.


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LAGM2020

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