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Psilosopherr
A psilly goose

Registered: 02/15/12
Posts: 12,284
Last seen: 7 months, 29 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: blazedup]
#25740453 - 01/12/19 06:46 PM (7 years, 9 months ago)

you do anything special to get ovoid to germinate? I seem to remember reading about others having trouble with this.

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blazedup
Dirk Diggler

Registered: 06/05/11
Posts: 1,244
Loc: Middle Earth
Last seen: 1 month, 23 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Psilosopherr]

1
#25740461 - 01/12/19 06:49 PM (7 years, 9 months ago)

Psilosopherr said:

you do anything special to get ovoid to germinate? I seem to remember reading about others having trouble with this.

Nothing special..it was my first time using a loop. Maybe i just got lucky by using too much for one plate

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woofwoof
such mushrooms!

Registered: 01/04/19
Posts: 1,127

Re: AGAR ENVY! (Anything and All things agar!)

[Re: blazedup]
#25740568 - 01/12/19 08:00 PM (7 years, 9 months ago)

blazedup said:

Tormato said:

blazedup said:

The full plate is rw and the other ovoid

You got some really nice rhizo growth in those red plates...try to transfer a small slice of one of those strands.

Thanks...do you suggest a specific side or any would do?

Take from the most
rhizomorphic area

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Lassy123

Registered: 12/22/17
Posts: 89
Last seen: 5 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: woofwoof]
#25741118 - 01/13/19 02:43 AM (7 years, 9 months ago)

Cloned a mushroom from a B+ grow that i liked.
I've never cloned before. I was wondering how much further i should take this.
Or just take it to grains now?

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woofwoof
such mushrooms!

Registered: 01/04/19
Posts: 1,127

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lassy123]
#25741142 - 01/13/19 03:23 AM (7 years, 9 months ago)

That looks pretty solid! Those sectors are nice, how many transfers did it take to get to that point?

Edited by woofwoof (01/13/19 03:23 AM)

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woofwoof
such mushrooms!

Registered: 01/04/19
Posts: 1,127

Re: AGAR ENVY! (Anything and All things agar!)

[Re: woofwoof]
#25741143 - 01/13/19 03:25 AM (7 years, 9 months ago)

Gunna be doing some agar for first time this week, using syringe. Agar seems exciting

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Lassy123

Registered: 12/22/17
Posts: 89
Last seen: 5 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: woofwoof]
#25741157 - 01/13/19 03:43 AM (7 years, 9 months ago)

1 transfer. It's pretty cool stuff. I just dont know enough about it. I mean i can get clean transfers but I've never cloned before.

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Edited by Lassy123 (01/13/19 03:44 AM)

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woofwoof
such mushrooms!

Registered: 01/04/19
Posts: 1,127

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lassy123]
#25741161 - 01/13/19 03:49 AM (7 years, 9 months ago)

Roger Rabbit has a video on strain isolation using agar and mushroom tissue for cloning which is pretty awesome

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MH5109
Stranger

Registered: 09/28/17
Posts: 1,385
Loc: America

Last seen: 5 years, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: woofwoof]
#25741178 - 01/13/19 04:12 AM (7 years, 9 months ago)

A couple AA+ plates that have some cool rizo growth.

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Failboat
Free man

Registered: 02/01/18
Posts: 8,741
Last seen: 9 days, 31 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lassy123]
#25741290 - 01/13/19 07:43 AM (7 years, 9 months ago)

Lassy123 said:

1 transfer. It's pretty cool stuff. I just dont know enough about it. I mean i can get clean transfers but I've never cloned before.

Peel a mushroom open to expose deep tissue. Use clean tool of your choice to remove a sample. Place tissue sample on agar. Transfer as usual.

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Caps McGee
Grandaddy Smurfshack

Registered: 10/28/17
Posts: 14,366
Loc: ally known as ...

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Failboat]
#25741641 - 01/13/19 11:36 AM (7 years, 9 months ago)

Use it, pick a fruit from the next run to clone, and isolate that way... you'll know exactly what you're getting then

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Lassy123

Registered: 12/22/17
Posts: 89
Last seen: 5 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Caps McGee]
#25742278 - 01/13/19 06:20 PM (7 years, 9 months ago)

but that would be cloning a clone right? That plate is already a clone. I hear thats not good to do.

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Caps McGee
Grandaddy Smurfshack

Registered: 10/28/17
Posts: 14,366
Loc: ally known as ...

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lassy123]
#25742370 - 01/13/19 07:24 PM (7 years, 9 months ago)

I've done it with 3 generations on agar before going to grain... awesome culture... a clone will still contain multiple strains, and a wide array of genetics... it's not advised to continue this way, once you have an isolate, slant it for cold storage and expand from it for several months, even a year or 2 of it's kept clean and given 24-48 hours to recover each time a transfer is taken...

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Tormato
Chairman of the Bored

Registered: 07/01/17
Posts: 6,147
Last seen: 1 month, 15 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lassy123]
#25744119 - 01/14/19 07:22 PM (7 years, 9 months ago)

Lassy123 said:

but that would be cloning a clone right? That plate is already a clone. I hear thats not good to do.

You can clone a clone just after numerous generations they can get funky. :shrug:

:shrug:

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Failboat
Free man

Registered: 02/01/18
Posts: 8,741
Last seen: 9 days, 31 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#25744172 - 01/14/19 07:55 PM (7 years, 9 months ago)

Just ordered 500 petris in sleeves of 20. Gonna be here Thursday. :awesome:

:awesome:

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mushroomnate
Pstranger

Registered: 05/17/17
Posts: 3,100
Last seen: 30 days, 21 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Failboat]
#25744177 - 01/14/19 07:57 PM (7 years, 9 months ago)

:highfive1:

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Tormato
Chairman of the Bored

Registered: 07/01/17
Posts: 6,147
Last seen: 1 month, 15 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushroomnate]
#25744179 - 01/14/19 07:57 PM (7 years, 9 months ago)

mushroomnate said:

:whathesaid:

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Helpful Threads

The Shroomery Store
Tormato's Q&A Thread Post Questions Here or PM me!
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Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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blazedup
Dirk Diggler

Registered: 06/05/11
Posts: 1,244
Loc: Middle Earth
Last seen: 1 month, 23 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#25744328 - 01/14/19 09:20 PM (7 years, 9 months ago)

Sneaky $h**.....

Womp womp...in the garbage

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Failboat
Free man

Registered: 02/01/18
Posts: 8,741
Last seen: 9 days, 31 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: blazedup]
#25744334 - 01/14/19 09:22 PM (7 years, 9 months ago)

Take a transfer first.

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Tormato
Chairman of the Bored

Registered: 07/01/17
Posts: 6,147
Last seen: 1 month, 15 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Failboat]
#25744336 - 01/14/19 09:22 PM (7 years, 9 months ago)

Quirkmeister92 said:

Take a transfer first.

:whathesaid:

--------------------
Helpful Threads

The Shroomery Store
Tormato's Q&A Thread Post Questions Here or PM me!
"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead
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