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Offlinesexymoreno
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Registered: 08/04/05
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Using agar to inoculate liquid culture.
    #4583078 - 08/26/05 05:10 AM (11 years, 3 months ago)

HOw would i do this?

Im thinking dissolve the agar culture into some sterile water then inject it into the jar. I'll be taking chances though.


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Invisibleagar
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Re: Using agar to inoculate liquid culture. [Re: sexymoreno]
    #4583085 - 08/26/05 05:38 AM (11 years, 3 months ago)

In a glove box, or flowhood just slice a colonized wedge out, open the jar & drop the wedge in, then close the jar. Or, in a glove box, or flowhood add a little sterilized water with a syringe - over the top of the agar culture surface, use the syringe needle to scuff up the myc into solution, asperate some up into the syringe & inject into the LC.


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Offlinesexymoreno
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Registered: 08/04/05
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Last seen: 10 years, 7 months
Re: Using agar to inoculate liquid culture. [Re: agar]
    #4583094 - 08/26/05 05:57 AM (11 years, 3 months ago)

THANK YOU SO MUCH!!!!x10 to the exponent of infinity!!!


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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