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Re: Official Copelandia/Panaeolus Thread [Re: Wedgie] * 1
    #29702379 -

Wedgie said:

WEZA x PAN IND01 I am loving how these are coming out...
but hmm, I dunno...
:aweshit:
It seems normal for WEZA but fast-pins don't seem typical for INDO1 It's still pretty early though my friends 🥳
So I will bring food 🍔 you guys get the drinks while we wait 🍻



Love the funky casing:mushroom2:


--------------------
It’s not personal

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Re: Official Copelandia/Panaeolus Thread [Re: idontknow1] * 2
    #29702478 -

Updates have been posted to my journal:

https://www.shroomery.org/forums/showflat.php/Number/29702148#29702148

I'm really pushing the limits of what pans can tolerate with this one.

After pasteurization, my casing only showed a pH of 7.2. Either my meter is wrong or I didn't add enough hydroxide. Only one way to find out.

Also, I think my substrate was a bit on the wet side leading to less vigorous colonization.

Either way, you can't un-ring a bell so I'm running it to see what happens.

I'm planning to spawn more jars tomorrow, so there will always be another battle. Hopefully after a few more runs I'll have a method that is repeatable and consistent which will help me test variables better.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Copelandia/Panaeolus Thread [Re: PuffM4gicDr4gon] * 4
    #29702481 -

PuffM4gicDr4gon said:
Updates have been posted to my journal:

https://www.shroomery.org/forums/showflat.php/Number/29702148#29702148

I'm really pushing the limits of what pans can tolerate with this one.

After pasteurization, my casing only showed a pH of 7.2. Either my meter is wrong or I didn't add enough hydroxide. Only one way to find out.

Also, I think my substrate was a bit on the wet side leading to less vigorous colonization.

Either way, you can't un-ring a bell so I'm running it to see what happens.

I'm planning to spawn more jars tomorrow, so there will always be another battle. Hopefully after a few more runs I'll have a method that is repeatable and consistent which will help me test variables better.



Your reader is probably not accurate.  60ml is about 1 tsp of lime per dry cup of peat and that’s plenty.  I wouldn’t add any more

If you want a true accurate ph reading, you need to get a somewhat nice digital reader that comes with calibration liquids (7 and 4 ph for example) and calibrate it often.  But if all you’re using it for is the casing, I’d just stick with the 1 tsp per cup of peat and call it good.. it hasn’t failed me in many years of using the same recipe


BVI tray that keeps giving up the goods.  Fifth flush


It’s starting to get cooler here and I want to run 6 trays during the winter.. 3 in the jcm and then 3 ziplocks.  I don’t want to have to heat the room tho so I’m trying out a reptile heating pad. 

The ziplocks are sitting directly on the pad.  My question about it is whether it’s the substrate/surface alone that needs to be at pan fruiting temps or does the actual air inside the ziplock need to be at that temp as well?  I assumed the air would follow close behind the sub temp.

There’s a thermostat with probe connected to the pad.  The only way (at least starting out) to fully control the sub temp with the probe is to stick the probe end fully into one of the subs



I have the temp set to 81F and there’s a 3 degree buffer so when the temp target is hit, it shuts off until the temp reaches 78F.  So far it seems to be working great. 

I’ve used a laser temp gun to read the surface of other ziplocks when I’ve had the heat turned off for like 12 hours and the ziplocks without the pad are reading significantly lower than the ones on the pad.. which are reading right in that target temp of 78-81F

Knowing that the temp of the air inside the bag moves 2.5F lower than the sub temp is useful because then I can set the probe target temp at say, 78.5F and I wouldn’t have to stick the probe directly into the substrate of one of the three trays. Not that I mind putting the probe into the substrate.  I sanitized it and it’s metal

I lasered the pad itself and doesn’t get above 90F so I’m not too worried about overheating the bottom part of the sub that’s closest to the pad.  But I’ve only had this set up for a couple days so I guess I’ll see if there’s anything like that that pops up

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29702557 -

fahtster said:
It’s starting to get cooler here and I want to run 6 trays during the winter.. 3 in the jcm and then 3 ziplocks.  I don’t want to have to heat the room tho so I’m trying out a reptile heating pad. 

There’s a thermostat with probe connected to the pad.  The only way (at least starting out) to fully control the sub temp with the probe is to stick the probe end fully into one of the subs


I have the temp set to 81F and there’s a 3 degree buffer so when the temp target is hit, it shuts off until the temp reaches 78F.  So far it seems to be working great. 

I’ve used a laser temp gun to read the surface of other ziplocks when I’ve had the heat turned off for like 12 hours and the ziplocks without the pad are reading significantly lower than the ones on the pad.. which are reading right in that target temp of 78-81F

Knowing that the temp of the air inside the bag moves 2.5F lower than the sub temp is useful because then I can set the probe target temp at say, 78.5F and I wouldn’t have to stick the probe directly into the substrate of one of the three trays. Not that I mind putting the probe into the substrate.  I sanitized it and it’s metal




Is evaporation a problem with the heating pad and the ziplocks? Would you rehydrate while fruiting or between flushes?

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29702589 -

fahtster said:
It’s starting to get cooler here and I want to run 6 trays during the winter.. 3 in the jcm and then 3 ziplocks.  I don’t want to have to heat the room tho so I’m trying out a reptile heating pad.




How cold does it get in the room? Temps ranges of between 12-20C (night/day) wold still work here but growth speed was diminish to almost that of regular cubes.

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 1
    #29702612 -

Thanks faht! I got the 1tsp per cup ratio from your tek. I didn't use quite as much verm because I didn't want to eyeball an extra cup so I did 2 quarts instead.

I did not use a bag to shake, to my own turmoil. Stiring the bucket released a death cloud right in my face. Definitely going to use one next time.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Copelandia/Panaeolus Thread [Re: PuffM4gicDr4gon]
    #29702648 -

shroboo said:
Is evaporation a problem with the heating pad and the ziplocks? Would you rehydrate while fruiting or between flushes?



Like I said, this is very new as I’ve only run it for a few days but that’s something I’ll be on the look out for.  The pad never gets above 90F which is about the highest temps at the height of summer but the probe is basically stuck into the sub all the way to the bottom so the sub itself never goes above 81F which is normal pan temps.  So, I’m not expecting crazy water loss but we’ll see.  I rehydrate between flushes anyhow.

Baba Yaga said:
fahtster said:
It’s starting to get cooler here and I want to run 6 trays during the winter.. 3 in the jcm and then 3 ziplocks.  I don’t want to have to heat the room tho so I’m trying out a reptile heating pad.




How cold does it get in the room? Temps ranges of between 12-20C (night/day) wold still work here but growth speed was diminish to almost that of regular cubes.



It can get really cold in the middle of winter but I do have an individual room wall heater.  The problem is that in order to keep the room in the mid 70’s, the heater pretty much runs constantly.  This gets expensive and the fan on the heater running continuously throws off the fae for the bags drying them out.

The goal is to keep the room in the 60’s while maintaining the pan sub temps in that 78-81F range.  This will still cause the heater to run in the room but not nearly as much.

PuffM4gicDr4gon said:
Thanks faht! I got the 1tsp per cup ratio from your tek. I didn't use quite as much verm because I didn't want to eyeball an extra cup so I did 2 quarts instead.

I did not use a bag to shake, to my own turmoil. Stiring the bucket released a death cloud right in my face. Definitely going to use one next time.



You can definitely vary the amount of verm np.. I’ve done 60/40 (what I used for cubes) and 50/50 (what I currently use for pans)

As far as the lime goes, I’ve used less as well (down to 1/2 tsp per cup of peat) and it works.  I did a lot of experimenting with lime amounts (that’s when I bought the digital ph reader) and what I found was that I didn’t like the way the myc wouldn’t grow into the casing some with lower amounts of lime.

Idk how you water your casing when you apply it but I basically flood the casing to the point of starting to pool.  I believe this is leeching off some of the lime from the casing and that water is being absorbed by the sub itself. 

What brought about me experimenting with it was that my buddy that grows pans a lot said that 1 tsp per cup was too much lime.  I had already been using the recipe successfully for years at this point and it worked great so I wanted to find out what happened when I used less and like I said, using less caused the myc to avoid growing into the casing at all.  I didn’t like that.  It left the casing loose on the surface and didn’t improve the flush at all.

So either pans like a higher ph in the casing or the heavy misting was diluting the lime down to where the myc likes it.  Either way, the 1 tsp per cup of peat works

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 1
    #29702651 -

Good to know!

I normally mist the hell out of it when I apply the casing so it's glistening with pools that dissappear. I did not do it this time because I forgot. I'll do that now.

Edit:

I'm glad you mentioned it. Each tray took about 300ml each. That definitely would've caused me some problems since I'm going to neglect this for a week.



--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Edited by PuffM4gicDr4gon (10/19/26 09:53 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: PuffM4gicDr4gon]
    #29702724 -

Working on some tests for Pan Bisporus

Love the species

Have some agar drops into coffee supplemented RGS

And a new concept mix that's more like potting soil

3 pints RGS (with one pot spent grounds, 1/8c coffee
Simmered with minimal water and few Tbsp gypsum

1 pint loamy hpoo (years old, maybe 5% sandł

1 pint perlite (fluffed into the hpoo)

1 pint Coarse verm

RGS is drained in strainer, but kept wet. Quick prep

1/3 folded into hpoo mix, then another 1/3

Last 1/3 is mixed with the verm, then both folded together to keep flufiness

16 pints at 60ish percent filled (23L Presto fill)

PC 17 psi for 90 min

Agar drops

Making a Journal for experiment and secondary spawn expansion and/or tray options

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29702837 -

fahtster said:
Baba Yaga said:
fahtster said:
It’s starting to get cooler here and I want to run 6 trays during the winter.. 3 in the jcm and then 3 ziplocks.  I don’t want to have to heat the room tho so I’m trying out a reptile heating pad.




How cold does it get in the room? Temps ranges of between 12-20C (night/day) wold still work here but growth speed was diminish to almost that of regular cubes.



It can get really cold in the middle of winter but I do have an individual room wall heater.  The problem is that in order to keep the room in the mid 70’s, the heater pretty much runs constantly.  This gets expensive and the fan on the heater running continuously throws off the fae for the bags drying them out.

The goal is to keep the room in the 60’s while maintaining the pan sub temps in that 78-81F range.  This will still cause the heater to run in the room but not nearly as much.





Yeah, costs of heating are the reason why I shut down during winter. I can manage to grow from mid spring to mid autumn. I'm curious to see how it
works out for you fruiting in the mid 60s without any addons just because your grows are so consistently well performing which makes it intresting to
see how changing one variable such as temperature would reveal it's influence more accurately I assume opposed to trying this with grows that
are mediocre and inconsistent or have you already had your share with fruiting in these temp ranges? Not trying to tell you what to do but since
I had OK results between 53F and 68F I'm really curious about your experience.

Edited by Baba Yaga (10/19/26 02:39 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #29702848 -

What ever happened to NecD? I bought two prints a few years ago when they dropped but never grew them out and haven't really looked around the forums for them since. I just found the prints a few weeks ago and germ'd a few plates, but after a bunch of searching I can't really find anything recent on them around the forums. Did they turn out to be meh?


--------------------

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #29702883 -

Baba Yaga said:
fahtster said:
Baba Yaga said:
fahtster said:
It’s starting to get cooler here and I want to run 6 trays during the winter.. 3 in the jcm and then 3 ziplocks.  I don’t want to have to heat the room tho so I’m trying out a reptile heating pad.




How cold does it get in the room? Temps ranges of between 12-20C (night/day) wold still work here but growth speed was diminish to almost that of regular cubes.



It can get really cold in the middle of winter but I do have an individual room wall heater.  The problem is that in order to keep the room in the mid 70’s, the heater pretty much runs constantly.  This gets expensive and the fan on the heater running continuously throws off the fae for the bags drying them out.

The goal is to keep the room in the 60’s while maintaining the pan sub temps in that 78-81F range.  This will still cause the heater to run in the room but not nearly as much.





Yeah, costs of heating are the reason why I shut down during winter. I can manage to grow from mid spring to mid autumn. I'm curious to see how it
works out for you fruiting in the mid 60s without any addons just because your grows are so consistently well performing which makes it intresting to
see how changing one variable such as temperature would reveal it's influence more accurately I assume opposed to trying this with grows that
are mediocre and inconsistent or have you already had your share with fruiting in these temp ranges? Not trying to tell you what to do but since
I had OK results between 53F and 68F I'm really curious about your experience.



I’ve fruited in the low 70’s np.  It’s definitely slower but I haven’t tried lower than that.  This is really just to get a feel for whether or not it’s even a viable option.  The real test will be in about a month when the temps really drop outside.  I do think that as long as I keep the substrate itself in the proper range, I won’t have too many problems.  I’ll be running a humidifier in the room  as well to help keep the rh near 60% in the room.  The only thing I foresee rn being an issue to contend with is condensation.  We’ll see what happens :thumbup:

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29702894 -

okay, I need the master's help.

Here are the Clones i took on the water agar from that bacterial tub.

Baba stated to use 0.5% nutrient agar. I'm going to whip some up really quick. but while i was doing that i wanted to ask you guys what you think about the plates so far.


There are some spots where you can see darkening 'veins' but the leading edge is very clean still so i'd be taking rice grain size transfers to low nutrient plates. Grow them out a bit more then put them in a cabin.


There is only one spot that has ariel myc and thats 2 o'cock above the right side clone on plate 1. I know these aren't the greatest looking but this is a full rescue attempt because i got no spores at all and the genetics i got from the vendor came bacterial.

Any spots that look better than others to any of you?

Plate 1



Plate 2



Plate 3



Plate 4


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

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Re: Official Copelandia/Panaeolus Thread [Re: Lo-Z]
    #29702931 -

My 2 cents:

Plate 1 has already sporulating mold on it. Transfer from plate 2-4 leading edge and put straight through a cabin and put some on low nute agar and or
pucks to keep options open. All the plates have this amber discoloration which is either a reaction to mold or bacteria or both. Well as plate one
shows there is definitely a pin mold at work. The other ones seem to be affected as well. All cultures "sweat" around the center which is an
indicator that there is probably mold running along, you can see a secondary growth pattern too. You could also try a hot pour over the plates
once you have taken the transfers which might help with the mold.


So,

  • take transfers to low nute and or puks and some through a cabin but .

  • Try a hot pour over one or two plates (choose from plate 2-4) I actually think in this situation it would be your best shot.

  • Keep any remaining original plates around to see what might pop up later on.

  • I would discard plate 1



This is water agar right? I might be wrong since I haven't used it in a while, just seems like a lot of growth for no nutrition. Are you using just plain agar powder?

Edited by Baba Yaga (10/19/26 04:41 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #29702993 -

Baba Yaga said:
My 2 cents:

Plate 1 has already sporulating mold on it. Transfer from plate 2-4 leading edge and put straight through a cabin and put some on low nute agar and or
pucks to keep options open. All the plates have this amber discoloration which is either a reaction to mold or bacteria or both. Well as plate one
shows there is definitely a pin mold at work. The other ones seem to be affected as well. All cultures "sweat" around the center which is an
indicator that there is probably mold running along, you can see a secondary growth pattern too. You could also try a hot pour over the plates
once you have taken the transfers which might help with the mold.


So,

  • take transfers to low nute and or puks and some through a cabin but .

  • Try a hot pour over one or two plates (choose from plate 2-4) I actually think in this situation it would be your best shot.

  • Keep any remaining original plates around to see what might pop up later on.

  • I would discard plate 1



This is water agar right? I might be wrong since I haven't used it in a while, just seems like a lot of growth for no nutrition. Are you using just plain agar powder?




Thank you Baba, and yes water agar. 2.5% agar (telephone brand) with blue coloring to remind myself that it is in fact water agar.

Plate 1 definitely has something going on and I suspected that would be the case the moment that whole ass stipe fell on the plate.


I'll take transfers from 2-4 on the leading edge,
put some through cabins,
put some through agar wadge's island tek,
transfers to low nute,
and do hot pour on 2 of the remaining 3. We'll see what i can come up with and report back in about a week or so when things pan out... lmao no pun intended.


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Lo-Z] * 1
    #29703068 -

So if you have mold meshed in there is no easy option or guarantee to get rid of it, but you said it's your only option to save this culture. How
about asking for a print in the marketplace or I bet someone here will help you out?

While you wait on new spores you could try cleaning this up but mold is a bitch to get rid off, well at least it will give you lots of practice but
really, going back to spores would be the best bet and try to clean this up as entertainment and practice in the meantime. I'd think repeated early
transfers from the leading edge switching between low nute agar and water agar might help and as I said before maybe a hot pour might help if the
mold gets stunned more than the pan mycelium.

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #29703094 -

That would be awesome, though im not sure i have access to the market place yet because im a wee baby.

I just took 4-5 cabins, 1 trap door, 2 trenches and 6 straight to low nute transfers and 2 plates straight hot pour.

We'll see 🤷‍♂️

Practice is practice, If i can clean it awesome. If not it buys my time to get to the market place for a spore print.


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Lo-Z] * 1
    #29703099 -

I’m doing the exact same thing with my TTBVI, while waiting for some spores! I am sure someone will reach out if you ask on here. If I had any I would send you some pan prints. But there are a ton of amazing people, I’m sure you can find some.


--------------------
*Not a therapist - Do not take advice from me

Be nice. You are loved. You deserve happiness. You are not alone.

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Re: Official Copelandia/Panaeolus Thread [Re: Lo-Z] * 1
    #29703100 -

Lo-Z said:
That would be awesome, though im not sure i have access to the market place yet because im a wee baby.

I just took 4-5 cabins, 1 trap door, 2 trenches and 6 straight to low nute transfers and 2 plates straight hot pour.

We'll see 🤷‍♂️

Practice is practice, If i can clean it awesome. If not it buys my time to get to the market place for a spore print.



Whats the strain?


--------------------
what i've been growing

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Re: Official Copelandia/Panaeolus Thread [Re: arlo.arlo] * 1
    #29703121 -

JoeTherapist said:
I’m doing the exact same thing with my TTBVI, while waiting for some spores! I am sure someone will reach out if you ask on here. If I had any I would send you some pan prints. But there are a ton of amazing people, I’m sure you can find some.



arlo.arlo said:
Lo-Z said:
That would be awesome, though im not sure i have access to the market place yet because im a wee baby.

I just took 4-5 cabins, 1 trap door, 2 trenches and 6 straight to low nute transfers and 2 plates straight hot pour.

We'll see 🤷‍♂️

Practice is practice, If i can clean it awesome. If not it buys my time to get to the market place for a spore print.



Whats the strain?





:boombox2::rockon:

I would have offered but my newest prints are all around 3 years old.

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Shop: North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale


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