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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #29671329 -



Man am I excited for the Baba Yaga comeback :awedance:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 2
    #29671414 -

Yeah, I'm getting excited too, although I can't go in knee deep anymore but have room to play a little.

I have a drum in the garden which I had filled with all sorts of invasive plants/weeds like Agapanthus, Tradescantia zebrina, ivy, oxalis......
Filled it up with water, put lid on and watched it for two years, feeding it a couple of buckets of wood ash during the winter.

First the top was covered in white mold, which disappeared after a year. Then the lid got pushed up from plant matter rising to the top due to gas
buildup and now most of it has settled down and it smells like ruminant manure. Going to trial it on a few plants as a fertilizer before I spread
it to make sure no pathogens are hiding in there and of course the liquid is going to be pasteurized and used to hydrate coir for pan tubs.


Stinky business, took the plant matter out today




The pH of the stuff is 6 which is not too bad, maybe a little hydrated lime to bring it up a notch.




Got a sack of horse manure here as well to run control tubs.

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #29671436 -

Has anyone ever seen TTBVI form blobs?  I’ve never had this happen with pans:




Edited by GerryD (09/17/26 04:40 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: GerryD]
    #29671472 -

Looks cool.

Do they bruise? Could be a reaction to something but would be nice if it is a mutation that can be stabilized. Definitely take a clone.

Please update with how this tray is going to progress, whether its going to contam and what ever happens.

Edited by Baba Yaga (09/17/26 05:32 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #29671607 -

wow, 5yr pan LC still alive. very interesting. did u test other pan cultures a well or just ur thai pan? have u tested pan prints as well? wondering how long they still viable.
 
whats ur recipe for ur LC?

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #29671672 -

Baba Yaga said:
Yeah, I'm getting excited too, although I can't go in knee deep anymore but have room to play a little.

I have a drum in the garden which I had filled with all sorts of invasive plants/weeds like Agapanthus, Tradescantia zebrina, ivy, oxalis......
Filled it up with water, put lid on and watched it for two years, feeding it a couple of buckets of wood ash during the winter.

First the top was covered in white mold, which disappeared after a year. Then the lid got pushed up from plant matter rising to the top due to gas
buildup and now most of it has settled down and it smells like ruminant manure. Going to trial it on a few plants as a fertilizer before I spread
it to make sure no pathogens are hiding in there and of course the liquid is going to be pasteurized and used to hydrate coir for pan tubs.


Stinky business, took the plant matter out today




The pH of the stuff is 6 which is not too bad, maybe a little hydrated lime to bring it up a notch.




Got a sack of horse manure here as well to run control tubs.





It doesn't always have to be go big or go home, I would guess a lot of us don't need any more fruits so then play is were it's at :samus:

I suppose you've seen MojaveMyc's CV+ thread? My point being that it's sounds to me that you are making your own fertilizer in that drum :strokebeard:

Either way I'm here for it :popcorn:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: maensel] * 2
    #29671684 -

Yep have seen the CV+ thread and might do something along those lines but the drum juice will be first.

I made this cause I wanted to kill those weeds but then I thought I keep it and see what is going to happen and whether it is going to be useful.

Yep fruits usually go on the compost playing around and trying new things is what drives me most.

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Re: Official Copelandia/Panaeolus Thread [Re: maensel] * 3
    #29671686 -

maensel said:
wow, 5yr pan LC still alive. very interesting. did u test other pan cultures a well or just ur thai pan? have u tested pan prints as well? wondering how long they still viable.
 
whats ur recipe for ur LC?





I have around 10 pan LCs here of which most are form 2022 and 2023. Since I am doing a lot with LC I have used 1 year old LC quite often. The Thai Cambo culture is from 10/2021 and the oldest of them all, it's the first pan I grew.

I much better about the age of the cultures now since this one came back, still have to test it on agar and grow out to see if it has still the same resilience and vigor...it's the best clone I found back then.


You can create long term storage liquid media by either dropping a few agar wedges into sterile water like ReverendMyc does here or you make regular malt extract LC @ 0.1-0.2% in distilled water and inoculate. There should be a thread here somewhere talking about long term LC but I can't remember who has done a write up on it. Mateja had a good visual presentation on nutrient content and mycelium growth here.


If you are interested in the topic of long term storage then you can check these papers out. I hope the links still work, there is a lot of info out there.


Some research papers on long term storage of mycelial cultures:

    An example for comparison of storage methods of macro fungus cultures Schizophyllum commune

    Evaluation of different storage methods for rapidand cost-effective preservation of Botryosphaeria species

    Preserving cultures of wood-decaying Basidiomycotina using sterile distilled water

    Saving-fungal-Cultures-Sterile-Water-By-Harris

    Preservation of fungi in water (Castellani) 20 years

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #29671693 -

Baba Yaga said:
Yep have seen the CV+ thread and might do something along those lines but the drum juice will be first.

I made this cause I wanted to kill those weeds but then I thought I keep it and see what is going to happen and whether it is going to be useful.

Yep fruits usually go on the compost playing around and trying new things is what drives me most.



:nodofunderstanding:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #29671751 -

thankx baba, good stuff. will check out the links.

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #29672092 -

Baba Yaga said:
Looks cool.

Do they bruise? Could be a reaction to something but would be nice if it is a mutation that can be stabilized. Definitely take a clone.

Please update with how this tray is going to progress, whether its going to contam and what ever happens.



Nope, no bruising whatsoever and doesn’t smell like a mushroom.  I’m kind of perplexed and 99% sure I didn’t mix up the genetics with something else.

I’m thinking it must be a result of contamination from the spawn although that looked very healthy.  The substrate and casing were used for Pan Jams in the same tub and grew just fine (see below).  :shrug:


Edited by GerryD (09/18/26 11:49 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: GerryD]
    #29672104 -

GerryD said:
Baba Yaga said:
Looks cool.

Do they bruise? Could be a reaction to something but would be nice if it is a mutation that can be stabilized. Definitely take a clone.

Please update with how this tray is going to progress, whether its going to contam and what ever happens.



Nope, no bruising whatsoever and doesn’t smell like a mushroom.  I’m kind of perplexed and 99% sure I didn’t mix up the genetics with something else.

I’m thinking it must be a result of contamination from the spawn although that looked very healthy.  The substrate and casing were used for Pan Jams in the same tub and grew just fine (see below).  :shrug:






Maybe a mycogone contamination? They seem way bigger than the mycogone blobs I've encountered, but I know it can fuck up the fruits and cause some weird shit. I have a stinking suspicion that the "Enigma" cube mutation is tied to a mild-variety mycogone contamination of the original culture. The culture I worked with was really early on, before it was named. And it was the first culture I ever had to diagnose with mycogone.

Edited by ghiajake (09/18/26 01:46 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: ghiajake] * 5
    #29672482 -

So I finally got to use the steamer at work for a working batch. I went with a 2/3 coir to 1/3 sifted Black Kow substrate this time. 2 quarts of substrate, and 1 pint of spawn per bag. Figured I'd just let the bags colonize in the dry fruiting system.



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Re: Official Copelandia/Panaeolus Thread [Re: ghiajake] * 8
    #29672493 -

Couple more ziplocks


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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 3
    #29672496 -

fahtster said:
Couple more ziplocks





NOICE!

Edited by MushLuvR77 (09/18/26 08:20 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 3
    #29672497 -

fahtster said:
Couple more ziplocks







Faht always being like :freewilly:

:illjustgothrowup:

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Re: Official Copelandia/Panaeolus Thread [Re: ghiajake]
    #29672683 -

Hello guys, just a quick question... I have 2 pan cyan tubs finished with the first flush. it been the 5 day today and I haven't seen any new pins. it is horse manure, palm peat and vermiculite substrate with a thin purr verm casing layer. i did not dunk it for the second flush. i asked AI and it advised me not to dunk because it would soak the cake and stall the grow. what do you guys do? i thought the bottom water technique would be the best option since it involves not completely covering the cakes.i dont think misting it would be best because the casing is fully saturated.


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Re: Official Copelandia/Panaeolus Thread [Re: Grim11311]
    #29672724 -

I'd give it a good mist and wait a bit longer.

What is the temperature in your room?

Have you got photos of your first flush?

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #29673055 -

Baba Yaga said:
I'd give it a good mist and wait a bit longer.

What is the temperature in your room?

Have you got photos of your first flush?




the room is at 18 degrees but the tub is 22-23 degrees Celsius. no sorry don't have pics of the first flush. should i misy even though the casing is at field capacity and perfectly wet?

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Re: Official Copelandia/Panaeolus Thread [Re: Grim11311]
    #29673076 -

If the casing is still moist and the first flush was not crazy big then I guess you could just leave it and give it more time. I used just verm as a
casing and although it can work it is not easy to judge the moisture level.

Having said this, I feel like if you have not had to mist since you've set up the tub then I think it could do with more FAE but that is just me trying
to give advice from afar with no sensory input other than a photo.

How do your grows usually behave, is no second flush within 5 days an unusual thing?

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Shop: Mushroom-Hut Substrate Mix   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   MagicBag.co Certified Organic All-In-One Grow Bags


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