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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: KlarnEvil9] * 2
    #29253507 -

I have 66qt tub with two holes cut in it. I spray really good on the inside with 90% ISO and let it sit for a few minutes before I do the Agar.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ClearLakeBoy] * 2
    #29253510 -

There are some good threads around here with pointers to help prevent contam. I'll see if I can find them and post the links.

Edit:

Why start with agar

Improve your sterile technique

My SAB - Spitball Jedi

Bod's SAB Tek

How it should and shouldn't look




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"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ClearLakeBoy] * 1
    #29253515 -

^ That'll do.

70% alcohol is actually a better sanitizer than 90% but no worries, that's just splitting hairs.

No need to wait btw, with the walls wet and static from the tote, any contaminants are stuck to the sides and the air is still, almost instantly. -Ever been drenched in sweat 🥵 and close the window? You can feel how quick it becomes dead air - 🤣🤣🤣

As for isolating away from contaminants or cleaning up cultures... It's good practice to make transfers as soon as you see mycelium expanding & not wait for days. Takes more plates but easier to fix; at least until you get comfortable with your sterile procedure & get an eye for different baddies & proper growth. 👍🏻🫶🏻


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Chock full o' nuts. ☕

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ShitdickNancy]
    #29253556 -

ShitdickNancy said:
^ That'll do.

70% alcohol is actually a better sanitizer than 90% but no worries, that's just splitting hairs.

No need to wait btw, with the walls wet and static from the tote, any contaminants are stuck to the sides and the air is still, almost instantly. -Ever been drenched in sweat 🥵 and close the window? You can feel how quick it becomes dead air - 🤣🤣🤣

As for isolating away from contaminants or cleaning up cultures... It's good practice to make transfers as soon as you see mycelium expanding & not wait for days. Takes more plates but easier to fix; at least until you get comfortable with your sterile procedure & get an eye for different baddies & proper growth. 👍🏻🫶🏻


Thanks, good info. I am still learning. I started off really good and had some really good tubs. I have a few going now with some pretty good shrooms. I have had some Trenching tubs going on lately. I am trying to figure out why. They start off good and then start Trenching. I moved the new tubs to a different room to try to insolate from the Trenching tubs. I am guess that the baterial is Trenching the new tubs.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ClearLakeBoy] * 7
    #29253914 -



T1 from clone plate


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The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nocap]
    #29254000 -

This plate was taken from 2 sections of g2a where I could tell that the growth came from each grain.
I like that you can see a border, but there is a clean line where you see where they're getting along.



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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

HitchHiker's Guide
Identifying proper surface conditions

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sprnk] * 1
    #29254653 -

Life situation made me stop abruptly quite awhile ago.  I had a great ghost isolated that reproduced fantastic shrooms - alas, that is gone. :frown:.

I had 1 syringe stored in a fridge and hoped beyond hope it would be viable and I could start again. How long is normal to see some activity on an LME agar plate??

I am really worried I have nothing to actually work with.


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🅃🄴🄰🄼  🄲🄻🄸🄽🄶🅆🅁🄰🄿

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas] * 1
    #29254662 -

Welcome back!

I have heard of spore syringes remaining viable for years under refrigeration.  If you don’t have luck though, slide on over to the Marketplace and get a fresh spore print from one of the giveaways. :mushroom2:


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The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nocap]
    #29254673 -

Thanks!  I’ll keep an eye out.


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🅃🄴🄰🄼  🄲🄻🄸🄽🄶🅆🅁🄰🄿

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas] * 1
    #29254694 -

Id definitely give it the ol college try at least! I can't speak to syringe shelf life, but I've seen mention of people reviving some pretty old spore prints anyway. I hope it works out for ya though!


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Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #29254697 -

It's not the best way to archive.  See the cryo tek for next time.


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Psilocybe Taxonomy

Panæolus Species

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dr. Smoke]
    #29254856 -

Put a drop on 6 plates 48 hours ago - not seeing anything yet.

Yeah, I had to stop abruptly


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🅃🄴🄰🄼  🄲🄻🄸🄽🄶🅆🅁🄰🄿

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas] * 3
    #29254945 -

Ah, two days isn’t much at all. Give it another week even.


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The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas] * 2
    #29255118 -

Bobgas said:
Life situation made me stop abruptly quite awhile ago.....I am really worried I have nothing to actually work with.


Welcome back!
There are 3 giveaways in my sig line.


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--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #29255560 -




These are my first plates. I used LC to inoculate. I could use some advice as to where I should start trying to isolate and why. There is only one super obvious contamination in one of the plates. I appreciate any insight on my journey.


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Is not what we see or seem but a dream within a dream? -Edgar Allan Poe

:mushroom2:The Hitchhikers Guide to the Shroomery:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Harpo67]
    #29255566 -

I wish i wasnt at work so i had time to edit those and show you. You want the most organized, quick growth


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #29255570 -

tryptkaloids said:
I wish i wasnt at work so i had time to edit those and show you. You want the most organized, quick growth


I got you fam…


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The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Harpo67] * 1
    #29255577 -




These spots all look pretty good to take a transfer.

If you see similar looking spots on the plates in person, and the pics didn't do it justice, then I would take some additional transfers from those spots as well.


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The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nocap]
    #29255605 -

tryptkaloids said:
I wish i wasnt at work so i had time to edit those and show you. You want the most organized, quick growth


No rush. 😀

I only have 3 fresh plates for the 3 best spots. Making more plates up today/tomorrow as I just got my order today.

I assume there are at least some promising transfers.
nocap said:



These spots all look pretty good to take a transfer.

If you see similar looking spots on the plates in person, and the pics didn't do it justice, then I would take some additional transfers from those spots as well.


Thank you!

I was eyeing a couple of those areas as well. Certainly not all. Heading to farmers marker and getting something there that I'm going to clone as well. WTH


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Is not what we see or seem but a dream within a dream? -Edgar Allan Poe

:mushroom2:The Hitchhikers Guide to the Shroomery:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Harpo67] * 7
    #29255731 -



I love a classic cube.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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