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PhunGuyBob
Self-Taught Mycologist!

Registered: 04/13/25
Posts: 20

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Jrey16]
#29199610 - 04/26/25 12:46 AM (1 year, 4 months ago)

I’ve noticed that the Jack Frost strain tends to produce very thin rhizomorphic growth with minimal aerial mycelium. Even after multiple transfers, I haven’t been able to achieve the thick hyphal bundles I’m seeing in your second image. Was that plate a transfer from the first one? I’m wondering what I might be missing or doing wrong.

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Bob | PhunGuyBob
A guy on a mission to change the world one spore at a time!
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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 14,953
Loc: South Africa
Last seen: 1 day, 2 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: PhunGuyBob]
#29199643 - 04/26/25 02:13 AM (1 year, 4 months ago)

Post pictures of your plates and explain your recipe used.

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AZ Starman2112
Male Half of a Friendly Couple

Registered: 12/11/24
Posts: 2,640
Loc: A Steamy Bedroom With The Cool...
Last seen: 1 day, 19 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: PhunGuyBob]
#29199692 - 04/26/25 04:18 AM (1 year, 4 months ago)

PhunGuyBob said:

I'm not very familiar with the HBP strain but just wondering what the inoculation source was? I would have to imagine LC inoculation?

It was a MSS. I’m going to take a couple of A2A transfers from each plate early this coming week and see what happens from those. So far they look ok, just puffy.

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cybesa
Loving soul

Registered: 05/24/18
Posts: 542
Loc: Europe
Last seen: 26 days, 23 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Smellyhobbit]
#29200811 - 04/27/25 08:20 AM (1 year, 4 months ago)

This is not the place for negativity so I don't get what's wrong with Piggy. You rock man, and I love what you said about LC! Truly a 3D grow medium! Just make sure the agar you put in it is 100% clean and boom, no reason not to use it! :grin:

:grin:

--------------------
Be yourself. Don't hurt other beings, or expect hurt yourself. Love others, and expect love yourself.

Cybesa's Ultimate woodlover project

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PhunGuyBob
Self-Taught Mycologist!

Registered: 04/13/25
Posts: 20

Re: AGAR ENVY! (Anything and All things agar!)

[Re: cybesa]
#29201355 - 04/28/25 12:30 AM (1 year, 4 months ago)

Can someone please explain what the heck is going on here? This is a standard agar recipe of agar agar, malt powder, and yeast. It's definitely not contaminated because three of them came out this way and I transferred the others but wanted to keep this one to see if there were any changes over time which there were not. It was mixed thoroughly but colonized this way. I took pieces of the rhizomorphic growth from the two other donor plates that took this form and all 20 recipient plates colonized with rhizomorphic growth only. Any insight from shroomery's finest myco experts?

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Bob | PhunGuyBob
A guy on a mission to change the world one spore at a time!
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PhenoDreamers
hi lol

Registered: 09/02/13
Posts: 3,601
Loc: USA

Last seen: 1 hour, 56 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: PhunGuyBob]
#29201465 - 04/28/25 06:20 AM (1 year, 4 months ago)

How thin did you pour your plates and what's your recipe percentages?

Edited by PhenoDreamers (04/28/25 06:21 AM)

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MycCycle
Searching

Registered: 04/13/24
Posts: 1,714
Last seen: 1 year, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#29201864 - 04/28/25 02:49 PM (1 year, 4 months ago)

I need a reality check before I toss more plates away.
This is cobweb mold, right?

--------------------
= Pressure needed to reach 121C/250F, should you decide not to vent =
= Cabin Sequestering FAQ (to roof, or not to roof?) =

"Assumption is the mother of all fuckups"

Edited by MycCycle (04/28/25 03:08 PM)

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milkboy
Child

Registered: 05/01/21
Posts: 2,727

Re: AGAR ENVY! (Anything and All things agar!)

3
#29202020 - 04/28/25 05:51 PM (1 year, 4 months ago)

If that's cobweb then I'm spiderman

Pic tax

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Check your vector

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Infinite Monkeys

Registered: 09/01/21
Posts: 408

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Infinite Monkeys]

5
#29202160 - 04/28/25 09:58 PM (1 year, 4 months ago)

Infinite Monkeys said:

Even though I lost all of my spore prints and cultures.
I discovered a single popcorn grain when cleaning my jars,
I carefully transferred it from the jar it was in onto a plate.
The jar must be at least two years old, I swear to God.

Although it was unlikely, the grain is taking off! I'm very excited.

Here's the original plate

Transfers

How are the transfers looking?

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Smellyhobbit
Amateur Wizard

Registered: 04/01/22
Posts: 18,424
Loc: Booger Central
Last seen: 40 minutes, 9 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Infinite Monkeys]

2
#29202161 - 04/28/25 10:09 PM (1 year, 4 months ago)

Super good. Nice work reviving an old kernel man. Good stuff.

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LewDoja
mad $cientist, ganjacologist

Registered: 09/18/09
Posts: 4,849
Loc: A fictional tale, in fictional...
Last seen: 3 months, 25 days

Re: AGAR ENVY! (Anything and All things agar!)

1
#29202185 - 04/28/25 11:21 PM (1 year, 4 months ago)

MycCycle said:

I need a reality check before I toss more plates away.
This is cobweb mold, right?

Its been awhile but iirc storm trooper always had a funniness to the myc... not saying u have or have not mold/S.T. myc.

But definitely not Cobweb

Edited by LewDoja (04/28/25 11:22 PM)

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MycCycle
Searching

Registered: 04/13/24
Posts: 1,714
Last seen: 1 year, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: LewDoja]
#29202236 - 04/29/25 02:57 AM (1 year, 4 months ago)

That’s good to know, thanks Lew!
Guess I’ll send some and see what happens.

I’m starting to see that the mycelium on all the other plates start looking funny as well. At first I thought it might have been the colouring, but it’s acting weird all across the board, even those without the colouring.

I’ve either fucked up badly somehow, contaminating everything, or somethings off with the ELME, Agar or the water. Only thing I’ve done different with this batch as opposed to earlier ones has been dropping the temp to sterilise at 15-17psi and running a shorter cycle. Can’t imagine it’s the latter, as 35min should be good for 700ml in glass bottles.

If anyone has any thoughts, beside me being a dirty fuck, I'm all ears 😁

--------------------
= Pressure needed to reach 121C/250F, should you decide not to vent =
= Cabin Sequestering FAQ (to roof, or not to roof?) =

"Assumption is the mother of all fuckups"

Edited by MycCycle (04/29/25 03:12 AM)

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milkboy
Child

Registered: 05/01/21
Posts: 2,727

Re: AGAR ENVY! (Anything and All things agar!)

2
#29202403 - 04/29/25 08:15 AM (1 year, 4 months ago)

Maybe try adjusting the nutrition content in the agar or changing the source of nutrition

I have a PDA recipe if you'd like to try it.

--------------------
Check your vector

Gummies by milkboy
Milkboys Public Display of Affection
I'm a Semp

Edited by milkboy (04/29/25 08:17 AM)

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LewDoja
mad $cientist, ganjacologist

Registered: 09/18/09
Posts: 4,849
Loc: A fictional tale, in fictional...
Last seen: 3 months, 25 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: milkboy]

2
#29202429 - 04/29/25 08:47 AM (1 year, 4 months ago)

:whathesaid:

Also nutritional yeast is a good booster

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MycCycle
Searching

Registered: 04/13/24
Posts: 1,714
Last seen: 1 year, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: milkboy]
#29202464 - 04/29/25 09:23 AM (1 year, 4 months ago)

I'll get the yeast with my next order, Lew, and I'd love that PDA recipe of yours milkboy.

Will be pouring 30 plates of BRF plates today, with a 2% agar content, to see whether I can get something to happen here, before I get PD and yeast.

😁

--------------------
= Pressure needed to reach 121C/250F, should you decide not to vent =
= Cabin Sequestering FAQ (to roof, or not to roof?) =

"Assumption is the mother of all fuckups"

Extras:

Huskies
Boop More Snoots

Registered: 03/22/16
Posts: 2,382
Last seen: 2 hours, 9 minutes

Re: AGAR ENVY! (Anything and All things agar!)

5
#29202480 - 04/29/25 09:39 AM (1 year, 4 months ago)

I’ve made agar successfully with just gnocchi boil water, vermicelli boil water and even ramen boil water.

Haven’t tried regular pasta yet, maybe it’s not starchy enough.

Moral of the story is, you probably have something in your home you can use as dinner and starch base for agar.

Taman Negara on Vermicelli Agar

AJMF

A T2 plate of tentatively Yusky spreading

And the Original Yusky germ plate, after having buried the contaminant. I'm giving it time to consolidate in the hopes that it might've eaten it.

--------------------
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Edited by Huskies (04/29/25 09:53 AM)

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ForZ

Registered: 11/10/24
Posts: 7

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Huskies]
#29203607 - 04/30/25 11:08 AM (1 year, 4 months ago)

some cyan plates from LC T3 and T2 clone from last harvest. Also fastest clone from a FahtLoc harvest. They look fine right? planned to do some SSLC with them later.
Cheers

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LewDoja
mad $cientist, ganjacologist

Registered: 09/18/09
Posts: 4,849
Loc: A fictional tale, in fictional...
Last seen: 3 months, 25 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: ForZ]
#29203723 - 04/30/25 02:16 PM (1 year, 4 months ago)

0.5% lme....?

Extras:

ForZ

Registered: 11/10/24
Posts: 7

Re: AGAR ENVY! (Anything and All things agar!)

[Re: LewDoja]

1
#29203740 - 04/30/25 02:32 PM (1 year, 4 months ago)

1% LME/ 2% Agar this time for the cyans. The Shakti is on 2/2

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,584
Loc: Hollywoo

Last seen: 19 hours, 51 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]

3
#29203946 - 04/30/25 06:27 PM (1 year, 4 months ago)

x7x+ climbing the wall of the plate. It wants out.

--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (04/30/25 06:36 PM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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