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Churpee Choozle
Crusted Cultinator

Registered: 11/11/24
Posts: 2,955
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Huskies]

5
#29182288 - 04/08/25 12:04 PM (1 year, 6 months ago)

Ingeli T1 looking good. I guess. Never seen ingeli before irl :lol:

:lol:

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Jaegur
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Registered: 03/01/25
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Churpee Choozle]
#29182442 - 04/08/25 03:28 PM (1 year, 6 months ago)

Hi there, agar peeps! I'm incredibly new to doing Agar work, would love any thoughts on what to look out for on these plates and what to do next (transfer to new plates, set them on fire, etc.,). I know at least one looks contaminated, not sure on the others though. These are all from the same cluster of Z-Strain.

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cybesa
Loving soul

Registered: 05/24/18
Posts: 542
Loc: Europe
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Jaegur]
#29182540 - 04/08/25 05:37 PM (1 year, 6 months ago)

Hey! From my limited knowledge, I can say that:
#4 - Looks like the kind of contam that carries on with the mycelium. Extremely hard to get rid of, I am on my Transfer #7 on my Hawaii MS and it still looks like that.. it is like I can't get rid of it. :sad:

:sad:

#1 looks similarly contaminated: this kind of contam makes it very hard to see where you could transfer from as it all looks universally crazy.

#2 has a healthy grow at 5'o clock, transfer from there!

#3 looks like the same bizarre contam that is super sticky

#5 looks like it has contam in the middle but healthy grow at the sides, try to transfer from around 5'o clock again!

Good luck! I have loved agar ever since starting in December! :grin:

:grin:

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cybesa
Loving soul

Registered: 05/24/18
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: cybesa]

3
#29182542 - 04/08/25 05:39 PM (1 year, 6 months ago)

Some of my beautiful plates that took me a few transfers to get clean (the last one, the Golden Teacher still looks questionable :wowz:

:wowz:

:uhoh:

)

Jack Frost clone transfer #3:

Golden Mammoth MS transfer #4:

Enigma clone transfer #2:

Bluey Vuitton clone transfer #2:

Golden Teacher MS transfer #5:

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Edited by cybesa (04/08/25 05:40 PM)

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RoscoeReturns
We’ve outstayed our welcome

Registered: 02/13/18
Posts: 3,126
Loc: State of Confusion
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: cybesa]

4
#29182756 - 04/08/25 08:02 PM (1 year, 6 months ago)

TTBVI likes blue.

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Nichrome
-675 kPa

Registered: 12/17/18
Posts: 8,951

Re: AGAR ENVY! (Anything and All things agar!)

[Re: RoscoeReturns]

1
#29182848 - 04/08/25 09:27 PM (1 year, 6 months ago)

Do you know what chemical the blue is?

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Mr Piggy
Swine Flew

Registered: 09/29/11
Posts: 16,764
Loc: bioilliminicent©️

Re: AGAR ENVY! (Anything and All things agar!)

3
#29182868 - 04/08/25 09:57 PM (1 year, 6 months ago)

Yeah, blue.

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Smellyhobbit
Amateur Wizard

Registered: 04/01/22
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Re: AGAR ENVY! (Anything and All things agar!)

1
#29182872 - 04/08/25 09:59 PM (1 year, 6 months ago)

Mr Piggy said:

Yeah, blue.

:rofl:

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fungitobewith
Fungi To Be With

Registered: 07/15/07
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Re: AGAR ENVY! (Anything and All things agar!)

1
#29182875 - 04/08/25 10:01 PM (1 year, 6 months ago)

Mr Piggy said:

Yeah, blue.

Methylene blue lol

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RoscoeReturns
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Re: AGAR ENVY! (Anything and All things agar!)

1
#29183084 - 04/09/25 06:25 AM (1 year, 6 months ago)

Nichrome said:

Do you know what chemical the blue is?

Disodium Erioglaucine. The dye also contains propylparaben and propylene glycol.

--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?

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PuffM4gicDr4gon
Professional Coir Waster

Registered: 06/09/11
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: RoscoeReturns]

1
#29183136 - 04/09/25 08:16 AM (1 year, 6 months ago)

This is 100% circumstantial, so take what I say with a grain of salt, but I've had nothing but problems with Club House brand blue dye. I'll have to check the ingredient list, but it did have PG.

When using it, my cube plates wouldn't grow rhyzo no matter what I did. I tried josex poke, cabin sequestering, and a bunch of transfers. I switched to yellow one day out of boredom and all of a sudden I have rhyzo growth. I now don't use any dye, but it seemed like an interesting coincidence to me.

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AZ Starman2112
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Huskies]

1
#29183274 - 04/09/25 10:53 AM (1 year, 6 months ago)

Huskies said:

You've got aggressive myc, pat your back and enjoy

You can say that again! This is about 36 hours after transfer. You can even see in the original plates where I took the samples from the myc is even starting to grow into the cutout holes! Also the last 4 pics are of some plates I inoculated 12 days ago with spore prints and mss.

And I have to confess, I had to order some premade spawn jars. I am going away for a few days and just don’t have the time to make them this coming weekend. Not happy about it, because I have been having good results making my own jars, but it is what it is.

T1 Transfers at 36 hours and the plates they were taken from…

12 day old inoculations with spore prints and MSS as labeled…

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Huskies
Boop More Snoots

Registered: 03/22/16
Posts: 2,796
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]

1
#29183392 - 04/09/25 12:50 PM (1 year, 6 months ago)

I'll want a swab or wedge of those purple mystics when the time comes fyi!

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oktopus

Registered: 01/09/24
Posts: 814

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Huskies]
#29183533 - 04/09/25 03:21 PM (1 year, 6 months ago)

I don't think it's gonna be clean... :confused:

:confused:

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AZ Starman2112
Male Half of a Friendly Couple

Registered: 12/11/24
Posts: 2,640
Loc: A Steamy Bedroom With The Cool...
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Huskies]
#29183578 - 04/09/25 04:28 PM (1 year, 6 months ago)

Huskies said:

I'll want a swab or wedge of those purple mystics when the time comes fyi!

:awethumb:

I think I can make that happen! Last batch of PM I did those are probably my wife and I’s favorite. Great body buzz and good for sexy time!

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MachineElf226
Stranger

Registered: 08/12/24
Posts: 395
Last seen: 7 months, 13 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]
#29183761 - 04/09/25 07:14 PM (1 year, 6 months ago)

Is this little spot of cubes infected?

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Mr Piggy
Swine Flew

Registered: 09/29/11
Posts: 16,764
Loc: bioilliminicent©️

Re: AGAR ENVY! (Anything and All things agar!)

[Re: MachineElf226]
#29183772 - 04/09/25 07:17 PM (1 year, 6 months ago)

:shrug:

grow it out and find out.

--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿

New to cultivation or the shroomery? Observe the hitchhiker's guide
The Hitchhiker's Guide to the Shroomery

Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

Extras:

fungitobewith
Fungi To Be With

Registered: 07/15/07
Posts: 979
Loc: Rolling Stone, PA/Some Place C...

Re: AGAR ENVY! (Anything and All things agar!)

3
#29183799 - 04/09/25 07:48 PM (1 year, 6 months ago)

multi-tasking!

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MachineElf226
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Mr Piggy]
#29183928 - 04/09/25 09:35 PM (1 year, 6 months ago)

That little 0.5 cm good enough to go into a 4oz jar of sterile grain? I think I'll wait a bit. I've transferred cultures about 3 times already. I'm out of plates! So I'm like between a rock and a hard place I got no backups till they come in mail so I dunno.. Maybe I need to chance it and transfer it to a grain jar...

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rhizoRider
Mycorrhizally expanding

Registered: 12/24/13
Posts: 3,530

Re: AGAR ENVY! (Anything and All things agar!)

[Re: MachineElf226]

5
#29183935 - 04/09/25 09:41 PM (1 year, 6 months ago)

Got lucky off 2nd spore plate transfer of 5made, and IWL looks fire here im thrilled 🙌 :awedance:

:awedance:

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