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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight] * 1
    #28647632 -

LadysKnight said:
While brf agar is a solid recipe, I haven't found it to have much effect on bacteria. It is great for germinating spores tho. Brf cakes and pucks otoh, are known bacteria chokers.


Derp, I meant "BRF puck", for some reason I thought they were synonymous.

I've got a very bacterial clone that I transferred yesterday. Hopefully at least one of the transfers is contam-free, otherwise I'll try throwing it onto a BRF or wheat puck.

Edited by arnold582 (02/05/24 03:16 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28648310 -

Plate read, please?

First-ever LME + yeast plates.  For LAGM, I transferred  from my spore smear plate to this T1 yeast plate using a needle biopsy.  Transferred myc sample smaller than a grain of rice.  This is day two. The growth looks too much to me.  Mold?  I've never used yeast plates, don't know what to expect.  Thanks in advance.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA] * 2
    #28648311 -

I have growth like this on a T1 from a clone plate. Looks like cube myc trying to figure out who it wants to be when it grows up to me.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #28648330 -

Ya looks a bit like my cousin. He, too, wanted to be a garbage man when he was a young growth.

Agar tax, PE clone:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28648334 -

Smellyhobbit said:
I have growth like this on a T1 from a clone plate. Looks like cube myc trying to figure out who it wants to be when it grows up to me.


:rofl:  Let's see what it decides to do.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: stubb]
    #28648335 -

stubb said:
Ya looks a bit like my cousin. He, too, wanted to be a garbage man when he was a young growth.

Agar tax, PE clone:



Little toddlers.  They are always trouble, aren't they?  But wait until they are teenagers!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA]
    #28648817 -

So since I started doing pours, I've noticed that my mycelium has been on the wispier side.

They seem to be clean, they pin, no sign of mold, and I've done a few transfers. But even when I grab rhizo  looking fgrowth, I get more peach fuzz.

For example, this one started fine, but went like this





I'm at T2 with this one, but T1 seemes to be pinning.





Things that might be the case, please let me know:

1- I am dumb and this stuff is highly bacterial/ the sauce containers are NOT good enough for agar.

2- Low nutrients in the agar. It's actually just boil water from oats and agar. Maybe I should adapt my recipe, go back to use the pastyplates variety? Apparently you need to stir pour agar to not let the nutrients settle? oooops?

3- Colder temperatures. The room they are in is closer to 18ish degrees. Could that slow down growth or create lazier looking myc?

4- This is totally fine and nothing to worry about.

I put some plates on some millet jars, half plate each. They are taking forever it seems. How long do your agar jars take to colonize? I can fortunately see some growth, but its been a week.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Huskies] * 3
    #28649505 -

B+ clone taken from a big ol cluster



--------------------
Hitchhiker's Guide to the Shroomery

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA] * 1
    #28649740 -

SWBZA said:
Smellyhobbit said:
I have growth like this on a T1 from a clone plate. Looks like cube myc trying to figure out who it wants to be when it grows up to me.


:rofl:  Let's see what it decides to do.


They grow up so fast, don't they? The toddler, the brother and the original spore streak. I promise I won't use blue agar again

Original


The brother


The tiny toddler

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA]
    #28649802 -

SWBZA said:




How much blue dye did you use?! :rofl:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Phaethon] * 1
    #28649815 -

Phaethon said:
SWBZA said:




How much blue dye did you use?! :rofl:


Waaaaay too much. Actually, that is not the problem. My wife gave me some coloring powder she uses for icing of cakes. That stuff is like glitter. I used less than fits on the tip of a teaspoon handle, and I cleaned up blue from kitchen surfaces for a week. That stuff is like trich - you have no idea how it got to where it is, yet, there it is! I worked as carefully as I can but my hands were blue for days. It didn't wash off, I think the color disappeared as my skin got replaced through normal biological processes :eek: That stuff should be classified as a biological weapon.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] * 1
    #28649818 -

Hey ya'll, This is my first post on this holy grail of mushroom knowledge so I apologize in advance if I sound like a newb.
In any case, I currently have 2 agar plates of gt and b+ that were inoculated via LC syringes that were left over from grain jars that are about 85% colonized. Now I'll admit I used WAY too much LC, I realize a drop will do but it was the last of the LC and I couldn't aim well in my SAB.

Agar report please? Should I take a transfer from one of the white puffy bits? Thank you





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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shroombensis] * 1
    #28649902 -

Yes, sounds like a good plan.
And welcome to the thunderdome Shroomery!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #28649962 -

APE from a (purchased) MSS that had germinated... to basically an LC to agar. This is the first transfer.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shroombensis]
    #28650038 -

Shroombensis said:
Hey ya'll, This is my first post on this holy grail of mushroom knowledge so I apologize in advance if I sound like a newb.
In any case, I currently have 2 agar plates of gt and b+ that were inoculated via LC syringes that were left over from grain jars that are about 85% colonized. Now I'll admit I used WAY too much LC, I realize a drop will do but it was the last of the LC and I couldn't aim well in my SAB.

Agar report please? Should I take a transfer from one of the white puffy bits? Thank you







Excellent first post. Starting on agar early and ready to learn. I agree with LadysKnight. Start getting some transfers from the outer edges of those puff balls and watch for clean symmetrical growth. As you mentioned, for future, use as little liquid inoculant on the plates as possible. Then they will have more rooms to grow out away from the inoculation points on the germ plate and it will be easier to identify places to transfer from on the germination plate. And you can save the rest for more plates later. But you are good to get several transfers now and make your selections on the next plates.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pablotx]
    #28650056 -

lol your mss germinated inside of the syringe? Did you watch it germinate or when you recieved it, it already germinated?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhenoDreamers]
    #28650074 -

It was already germinated... and from one
SupaThaRipper said:
lol your mss germinated inside of the syringe? Did you watch it germinate or when you recieved it, it already germinated?


Already germinated - go figure. From one of the trusted vendors.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pablotx] * 1
    #28650079 -

Those dirty bitches sent you an LC 🤣

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhenoDreamers]
    #28650216 -

Are there any advantages to using MEA over PDA?

So far I've only been able to find PDA ingredients locally, so I only used that recipe. I finally got my hands on some LME so today I mixed it up for the first time, and something about its smell made me super nauseous. I always boil my mixture before PCing because my agar is very strong and if I don't dissolve it completely, it doesn't mix evenly in the bottle during the cycle and the bottom turns solid while the top 2/3rds don't thicken enough. The LME doesn't have a strong smell, but for some reason it almost makes me want to puke, something about it doesn't agree with me at all. So unless it works so much better than PDA, I'm never using that shit again.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LyleChipperson]
    #28650757 -

My t1 plates always grow out clean. Is it risky or bad practice to use these for inoculating grain rather than going to t2 first?


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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