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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Whiterabbit016]
    #28485821 -

Thanks all work was done in a still air box trying to be as sterile as possible. But yeah the condensation was pretty heavy on the PE Clone. Here's an up close pic and another with a light behind it. To my rookie eyes they look pretty darn good as well.


Edited by Chumbum (09/28/23 04:57 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SwabMarley] * 2
    #28485824 -

SwabMarley said:
Back up plate for @Fahts PE Clone :rockon:




Hell yeah!!
Gotta love Faht!!!

My Faht PE!!


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28486011 -



40 plates poured, 4 different recipes (10 of each).

Recipe 1 (bottom left)

200ml distilled water
3.0g agar
3.0g light malt extract

Recipe 2 (top left)

200ml distilled water
3.0g agar
3.0g casein hydrolysate

Recipe 3 (bottom right)

200ml distilled water
3.0g agar
3.0g light malt extract
0.1g nutritional yeast

Recipe 4 (top right)

200ml distilled water
3.0g agar
3.0g casein hydrolysate
0.1g nutritional yeast

It's an experiment. I have 2 clones, 6 various transfers and 2 germinations to do. For each inoculation I will repeat 4 times across each of the different recipes. This way I will be able to compare like for like for each of the recipes in different situations.

I poured them a little hotter than I usually do due to time constraints so there is probably a fair amount of condensation. I'll leave them in front of the flow hood until tomorrow and do the inoculations at about +20 hours.

Initial observations are that light malt extract is more orange where as the casein hydrolysate is very pale. Nutritional yeast makes the recipe cloudy. Casein hydrolysate is much nicer to work with than malt extract.


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Edited by covertjoy (09/29/23 12:40 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: covertjoy]
    #28486052 -

covertjoy said:
40 plates poured, 4 different recipes (10 of each).

Recipe 1 (bottom left)

200ml distilled water
3.0g light malt extract

Recipe 2 (top left)

200ml distilled water
3.0g casein hydrolysate

Recipe 3 (bottom right)

200ml distilled water
3.0g light malt extract
0.1g nutritional yeast

Recipe 4 (top right)

200ml distilled water
3.0g casein hydrolysate
0.1g nutritional yeast

It's an experiment. I have 2 clones, 6 various transfers and 2 germinations to do. For each inoculation I will repeat 4 times across each of the different recipes. This way I will be able to compare like for like for each of the recipes in different situations.

I poured them a little hotter than I usually do due to time constraints so there is probably a fair amount of condensation. I'll leave them in front of the flow hood until tomorrow and do the inoculations at about +20 hours.

Initial observations are that light malt extract is more orange where as the casein hydrolysate is very pale. Nutritional yeast makes the recipe cloudy. Casein hydrolysate is much nicer to work with than malt extract.


Why did you try the casein? It's a protein so your swapped a sugar for nitrogen... its not a different kind its an entirely alternative form of nutrition


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium]
    #28486286 -

These are transfers I took from agar I colonized with grains and a couple pins. The pins all look like mold. I had trouble so I'm not surprised ... anybody have good advice or a link to a page that gives good advice about cloning pins. It was harder than I thought. I couldn't get a piece to stick to my razor so I kept having to cut it and stab it over and over. Not so good... anyways the grain transfers look good to me what do you guys think? Should I do another transfer on the good ones or are they ready for grain. I can take individual pics if anyone sees anything they think I should address. Thanks in advance. The top one and bottom two pics are the good ones and all the others are the pins that sucked.






Edited by TrKyNcKmSk (09/28/23 10:41 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TrKyNcKmSk]
    #28486338 -

Is it normal for the mycelium to be aerial near the agar piece, or is that contamination or what. The petri dishes look funny compared to my holy Grail no pours, but i also cooled my blade all over in them, hence why it looks broken in some areas. What do y'all think? How should I proceed?





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Re: AGAR ENVY! (Anything and All things agar!) [Re: TrKyNcKmSk]
    #28486339 -

Here's another and the blade lines are more obvious.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium] * 1
    #28486344 -

MicroMycelium said:
Why did you try the casein? It's a protein so your swapped a sugar for nitrogen... its not a different kind its an entirely alternative form of nutrition


I don’t have any particular expectations, in this case I am just curious to see how the fungi behaves in different situations. I’ll repeat the same clones, transfers and germinations on all four recipes and observe the results.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: covertjoy]
    #28486345 -

Not sticking up like the Petrie dishes and they don't look funny even though there are huge razor gauges from cooling a red hot blade... What gives? Any advice would be appreciated. Do I need to keep transferring or are they clean?




Sorry for all the posts, I could only upload a couple pics at a time.

Edited by TrKyNcKmSk (09/29/23 12:34 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TrKyNcKmSk]
    #28486593 -

The aerial myc near the transfer piece is very common ime. Nothing to worry about. I believe that it is just the myc stretching out and "looking for" a hospitable place to grow before it finds the agar surface.

Not sure why you don't see in on the holy grails. It just may be harder to see due to less clarity?

It looks like you are cooling your blade near the center of the plate where the transfer goes. I recommend cooling your blade near the outer edge so it doesn't interfere with the growth. That just makes it harder to determine if you have nice symmetrical growth.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] * 1
    #28486687 -

I just cut my transfer with a hot scalpel. :shrug:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28486766 -

Same. I never understood the whole cooling it down thing


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Re: AGAR ENVY! (Anything and All things agar!) [Re: covertjoy]
    #28486770 -

you guys are too fancy...
I'm a basic dude:
400ml tap water
12gr Potato flakes
8gr Agar Agar
4gr Corn Syrup
some drops of food colouring


--------------------
β€œAll this buttoning and unbuttoning”

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28486772 -

tryptkaloids said:
I just cut my transfer with a hot scalpel. :shrug:


AspectOfTheCreator said:
Same. I never understood the whole cooling it down thing


You can certainly do it that way as well. I take tiny transfers and find that the hot scalpel melts the agar making it hard to see my target piece. Like  tiny little 1mm pyramids.


If I cool it in agar, I usually do it on the outer edge of the transfer plate rather than the receiving plate, but that may not be best practice since sending plate has a live culture and ideally the receiving plate is completely sterile until inoculated.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

Edited by ReverendMyc (09/29/23 02:16 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] * 4
    #28486925 -

Ghost clone tissue ready for grain


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Re: AGAR ENVY! (Anything and All things agar!) [Re: 999999999] * 1
    #28486932 -

Gleyck said:
you guys are too fancy...
I'm a basic dude:
400ml tap water
12gr Potato flakes
8gr Agar Agar
4gr Corn Syrup
some drops of food colouring


This guy fucks
:pointmade:


--------------------
Check your vector

Gummies by milkboy
Milkboys Public Display of Affection
I'm a Semp


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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos] * 1
    #28487107 -

tholos said:
Ghost clone tissue ready for grain




:halfcocked:
This thread will be the death of me


--------------------
β€œSunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
β€œThere is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28487185 -

0mar said:
tholos said:
Ghost clone tissue ready for grain




:halfcocked:
This thread will be the death of me


Those are some beautiful plates!!!


--------------------

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: HILLBILLY OUTLAW] * 2
    #28487605 -

Sent some tat v wombat swabs to new agar recipe (6gs LME/8gs Agar/400 ml distilled) only did one Z shaped streak to not have such messy plates. Most of my other plates look really moldy so I tossed most of my germ plates and only kept one ghost plate in hopes of pins… I haven’t gave up just yet folks. I’ll give it my all till I run out of spores :facepalm:


--------------------
β€œSunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
β€œThere is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar] * 3
    #28487611 -

And then when you run out we will send you more.

You’ve got it bro


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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