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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: NotFromGeorgia]
    #28445861 -

Hey I'm just curious how did you inoculate? Just shoot in a drop, or did you use an inoculation loop?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TrKyNcKmSk]
    #28445865 -

shot in 2 drops, some cups got 1 drop


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The most important step a man can take. It's not the first one, is it?
It's the next one. Always the next step, Dalinar.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: NotFromGeorgia]
    #28445869 -

I've been told that's bad and is way too much. I have not done it yet, but I will use an inoculation loop. Hopefully that may help you too. You can make homemade ones, bod has a tek or you can buy em, they're cheap.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TrKyNcKmSk]
    #28445872 -

Definitely use a drop on a loop and streak OR put a drop to a BRF puck and transfer to agar form that.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TrKyNcKmSk]
    #28445874 -

ya I made one but forgot to grab it. Was already gloved up in front of the SAB and said beginner's luck let's go! Turns out it doesn't work that way ha

That jmf syringe was growing ick spots faster than the lc syringe grew myc so I'm not sure if it's even worth doing anything with it

Smellyhobbit said:
Definitely use a drop on a loop and streak OR put a drop to a BRF puck and transfer to agar form that.


aye aye capn


--------------------
The most important step a man can take. It's not the first one, is it?
It's the next one. Always the next step, Dalinar.

Edited by NotFromGeorgia (08/24/23 10:30 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28445878 -

tryptkaloids said:
an old post from mushboy isn't gonna convince me


:mostinteresting:
how about a new one.

although it will be the same as the old one.

ive really only seen rings during the summer. im leaning towards temp these days. when i was a noob i thought it was light because night and day were the easiest variables to observe.

i honestly dont know.


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

Edited by mushboy (08/24/23 10:33 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: NotFromGeorgia]
    #28445887 -

:lol: you're SO convincing


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 1
    #28445888 -

Anyone have tips for germinating old improperly stored prints?

I'd atleast like to try a hail Mary or 2 before I deem them dead


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28445891 -

Careful now everyone. If you don't have plates going, you are flooding the forum!!! Or so I was told. ๐Ÿคช

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TrKyNcKmSk] * 2
    #28445925 -

Temperature fluctuations accentuated by winter, the out ring coincides perfectly with the turning on of an oil heater.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #28446005 -

I have these plates which all have rings forming at more or less the same time. Checked the temperature over the growth time and after one week around the 12.8 the temperature raised by about 10 degrees and stayed there. Definetely supports the theory of temperature fluctuations.
Either way im happy to hear this because i was thinking its a sign of bacterial contamination :rolleyes:



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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28446110 -

tryptkaloids said:
no need to yell. Dood.

That used to be said pretty often, yet I've never seen evidence of or seen anyone show a light cycle cause rings. I've also seen cultures in no light have rings.

I like to be proved wrong, but an old post from mushboy, (that you have to filter to his posts to find) isn't gonna convince me. I'll test it when I get a couple cultures going. And try and cause some with light just to see if I can.


Just making shur this info is available.
Because rings can be caused by light ๐Ÿ’ก
And your misinforming noobs

All i did was search rings on agar and click the small tc box pimp .
As should all your searches be before trying to correct some shit lol
But fr doe i asked this question about rings on agar a year or 2 ago .
And was told light

Edit: heres another tc. 
verum subsequentis said:
A lot of things can cause rings, including but not limited to; light, temp swings and contamination. My guess, in this particular situation, is contamination.



Edited by Culliganman360 (08/25/23 07:18 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Culliganman360]
    #28446227 -

My point is, a lot of things are taken at face value and passed on without evidence. TCS aren't infallible.

I'm sure they are better equipped to prove me wrong than I am at the moment, and I hope they do.

I hear your criticism and I'll do my best to not sell shit as shoeboxes.
But I, just like the TCS am human.

Mush love. :mushroom2:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28447026 -

Made a thread but didn't get much answers so I thought I'd ask here

I put some agar plates in the fridge 15 days ago, most of it were in tupperware but this particular one was just in a plastic baggie.
As you can see its all dried out and the agar is kind of folding over itself.
Now I'm paranoid and not sure if just tupperware is enough.
My fridge is 3ยฐC and it's not a dedicated myco fridge

How do you store your agar dish for maximum shelf life ?




Edited by enscred1312 (08/26/23 05:45 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 1
    #28447070 -

tryptkaloids said:
Anyone have tips for germinating old improperly stored prints?

I'd atleast like to try a hail Mary or 2 before I deem them dead


You can try rehydrating the print in sterile distilled water. Add a drop of surfactant before sterilization cycle, jet dry or polysorbate 20. After 24 hours, streak spore solution to agar.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: enscred1312]
    #28447089 -

enscred1312 said:
Made a thread but didn't get much answers so I thought I'd ask here

I put some agar plates in the fridge 15 days ago, most of it were in tupperware but this particular one was just in a plastic baggie.
As you can see its all dried out and the agar is kind of folding over itself.
Now I'm paranoid and not sure if just tupperware is enough.
My fridge is 3ยฐC and it's not a dedicated myco fridge

How do you store your agar dish for maximum shelf life ?






I'm my experience, plates aren't really meant for long term storage. I've had success for a few months with them in the fridge but after 3-4 months they start to dry out a little. If you want long term storage try slants or for really long term storage distilled water storage. If you just want to use plates you will need to transfer to new plates a few times a year.


--------------------
Pastywhyteโ€™s Ez dialed monotubs
My Miraculix Potency Test Results
The Hitchhiker's Guide to the Shroomery
My Museum





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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bigworm] * 1
    #28447118 -

It doesnโ€™t really matter if they dry out though does it? Kind of like dried grains except itโ€™s with agar?


--------------------
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


Help US help YOU TEK

2023 Dehydrator TEK

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bigworm] * 1
    #28447128 -

I store my plates for a year plus, then make fresh transfers. It really depends upon the quality of refrigerator, and the culture cleanliness.

Don't use beer fridges with internal freezers or you'll lose a bunch.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28447149 -

:whathesaid: In my experience storing in my mini fridge with a top separate feeezer the wrong spot in the fridge will still freeze plates quick. I stear clear of storing in it now.


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๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28447185 -

Screwup said:
It doesnโ€™t really matter if they dry out though does it? Kind of like dried grains except itโ€™s with agar?


Yup. I've revived a dried plate by chipping a piece of agar with myc on it and putting it on a new plate.

Stipe-n Cap said:
I store my plates for a year plus, then make fresh transfers. It really depends upon the quality of refrigerator, and the culture cleanliness.

Don't use beer fridges with internal freezers or you'll lose a bunch.


I'm using a keg fridge but no freezer. It dries my plates pretty quick. I see your point about freezing if they were in the wrong spot though.


--------------------
Pastywhyteโ€™s Ez dialed monotubs
My Miraculix Potency Test Results
The Hitchhiker's Guide to the Shroomery
My Museum





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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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