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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: stockw] * 2
    #28392156 -

You didn't take the whole culture, the mycelium is embedded in the agar and will continue to consume the nutrients and grow outwards - its normal


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smoothcat]
    #28392193 -

Makes sense, thanks man.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: stockw] * 1
    #28392434 -

Did similar with plates that were put to grain a few weeks ago just to see what they would do. They even started climbing over the top of the well and under the cling wrap :crazy:



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Re: AGAR ENVY! (Anything and All things agar!) [Re: MethodMyst] * 1
    #28392639 -

This could be the next step if you let the plates sit for longer :mushroom2:



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Re: AGAR ENVY! (Anything and All things agar!) [Re: MethodMyst]
    #28392874 -

MethodicalMystical said:
Did similar with plates that were put to grain a few weeks ago just to see what they would do. They even started climbing over the top of the well and under the cling wrap :crazy:




Where did you get those lovely little plates? What size are they?

stockw, I've had this happen also. It's normal at all stages for mycelium to be able to go from fluffy to rhizo and back.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #28392921 -

They look like 50mm.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28393078 -

They're 60mm from Celltreat. I ordered a case of 500 a while back (from eBay iirc, since it's about half the cost as the main site). Pretty neat for small form factor grows, but can take a bit to get used to if you typically use larger containers.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MethodMyst]
    #28393340 -

What does it mean to fully colonize an agar plate?

Is it just when mycelium covers the entire surface area regardless of its characteristics? Is there a benefit to waiting longer before using the plate?

I ask because I noticed multiple of my transfers seem to cover the entire surface quicklyโ€ฆ but itโ€™s typically very thin mycelium and it seems to thicken/continue developing even after โ€œfullyโ€ covering the surface.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium]
    #28393350 -

Sounds like mold.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28393361 -

Stipe-n Cap said:
Sounds like mold.


You think? This is an example of what Iโ€™m talking about.
The spread from the transfer is typically very thin like this, and once it reaches the edge of the plate, it will thicken up over the next week or two.
I was attributing it to my agar formula


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium]
    #28393387 -

What recipe are you using?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] * 2
    #28393400 -

Stipe-n Cap said:
Sounds like mold.


:whathesaid:
I'd even say it looks like mold


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Prolific Koi]
    #28393413 -

Prolific Koi said:
What recipe are you using?


Broad range medium total nutrition content.
ME, Yeast, Peptone, Charcoal, Agar

Iโ€™ve put one of these plates to grain and now substrate and itโ€™s colonizing the substrate fine so I highly doubt mold


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28393420 -

Mold colonizes sub too. Only time will tell what fruits


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 5
    #28393424 -

Doesnโ€™t charcoal fuck with growth? Why so complicated? LME AGAR WATER done


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Screwup]
    #28393437 -

Screwup said:
Doesnโ€™t charcoal fuck with growth? Why so complicated? LME AGAR WATER done


I have regular PDA plates running fine but I wanted to mix it up because I wasnโ€™t fond of their development.
This formula was obviously worse but thatโ€™s still information to use for comparison in the future.
The definition of insanity is doing the same thing over and over and expecting different results. -Albert Einstein.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium]
    #28393460 -

What is the best way to clean up โ€œscalloped mycelium?  Do I take a small piece from the flattest section?  Water agar?  Is the culture not salvageable?

This is a Ghost culture that seems to grow very quickly / well, and produces really nice fruits with good potency, so Iโ€™d like to see it remain viable over time.

Iโ€™ve cloned twice now and the plates are always a bit scalloped and flushes certainly could be more dense.  Spawn cleanliness is good, but not perfect - and I canโ€™t seem to get perfect regardless of oat prep and ridiculously anal inoculation in front of an FFU.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas] * 2
    #28393464 -

I feel like one of the TCs have said trying to clean up scalloped plates always ends up not working as if something is constantly riding along with it as they transfer. Maybe Iโ€™m wrong and it was something else with agar.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas] * 1
    #28393466 -

you got some pics Bobgas?

You could try a hot pour if you suspect bacteria, water agar would be worth a shot
and there is always the Josex style BRF plates


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium]
    #28393468 -

Transferred these on 6/4 and ended up using other plates. These have been just sitting on my desk since then. If you zoom in the second picture there all these little dots. What are those? Knots? Also, is it recommended to take off cling wrap if I want plins?



Edited by Stroms (07/12/23 12:00 PM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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