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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri] * 3
    #28384879 -

Give these a chance to grow and share results. I know I know i know the science behind it. If I cook my agar too long it will not grow anything, and that is just a bare, naked-assed fact. I dropped my cook time and it harbored life. I don’t know why, I’m not making any claims, I’m just putting forward a solution that worked for me. Doesn’t mean it’ll work for you.

Even water agar grows myc. There’s no straightforward reason why a handful of people, new or otherwise, get dead plates.

Being new doesn’t mean anything. I’m doing everything identically to when I first started except cook time 🤷♂️


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #28384889 -

Smellyhobbit said:
I know I know i know the science behind it.


You triple know the science? Teach me :hereyougo:

Sometimes I put the left foot in the right shoe so you better be prepared at the kind of student you are taking up here. Just saying, full disclosure.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: altford78] * 1
    #28384893 -

I use munchausen's agar recipe to the tea and never had issues germinating even the most stubborn of spores. It is weird that not even bacteria is growing...


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A Handy Dandy Rule Book for the Beginner Cultivator /WBS flash prep Tek /Bulk Substrate Coir Tek /Shoebox Tek /Bottle Tek /Clean spawn Checklist /Agar Recipe /Don't put Spores to Grain /Updated Pf Tek /Surface Conditions


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Re: AGAR ENVY! (Anything and All things agar!) [Re: altford78] * 2
    #28384913 -

altford78 said:
Smellyhobbit said:
I know I know i know the science behind it.


You triple know the science? Teach me :hereyougo:

Sometimes I put the left foot in the right shoe so you better be prepared at the kind of student you are taking up here. Just saying, full disclosure.


The only important thing is this:

Your PC chemically can’t caramelize the starch in your media. It doesn’t get hot enough to cause problems, so cook time shouldn’t matter at all. You physically can’t “overcook” your solution, so it shouldn’t matter if it’s in there longer than 30 minutes.

But I only have success between 20-30 minutes. That’s not a disputable issue. I don’t know WHY, but that is the truth. Like 5+ batches of dead plates with known, established recipes say so. Besides that, the recipes aren’t ever a problem. Agar + nutes + water = working plates. Everyone agrees on that. So why are we getting barren plates?


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 5
    #28384920 -

solution? just stop being a noob bro

:pointmade:


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you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #28384921 -

Wait, what? You also have barren plates? Serious?

I get plates full of LIFE at 15-18psi.



--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

Edited by altford78 (07/04/23 04:23 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: altford78] * 1
    #28384924 -

altford78 said:
Wait, what? You also have barren plates? Serious?

I get plates full of LIFE at 15-18psi.




Had. I was cooking for 90 minutes at 15psi and they would either grow nothing or myc would stall out at a dime sized diameter. Even contam, if it germinated, would stay pin prick sized.

Dropped it to 20-30 minutes and never had another problem.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28384939 -

:ohnowaitokay:

But why???? I actually believe you, but why would they not grow on 45min+ cooked media. :shrug:

Like what could possibly inhibity growth, it's just carbs one way or another.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: altford78] * 1
    #28384944 -

Literally have no clue


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28384951 -

Smellyhobbit said:
Literally have no clue


Wonder if sterilizing for too long denatures any of the nutrients? I know this is kind of a sore topic, but im genuinely interested. Have you ever gone back to pressure cooking them for longer and seen if its still not germinating any life, or tried different nutrients?


--------------------
A Handy Dandy Rule Book for the Beginner Cultivator /WBS flash prep Tek /Bulk Substrate Coir Tek /Shoebox Tek /Bottle Tek /Clean spawn Checklist /Agar Recipe /Don't put Spores to Grain /Updated Pf Tek /Surface Conditions


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28384953 -

Fuck me I hate these unknown unknowns :dipspit:


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Pandaskis] * 2
    #28384954 -

I said I was going to do a run following my old protocol last time it got brought up, but then I forgot lmao. I should. Worst case scenario the agar works and then we have no fucking idea what causes this phenomenon or how to fix it.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28384958 -

Smellyhobbit said:
I said I was going to do a run following my old protocol last time it got brought up, but then I forgot lmao. I should. Worst case scenario the agar works and then we have no fucking idea what causes this phenomenon or how to fix it.


Do it pleaseeee


--------------------
A Handy Dandy Rule Book for the Beginner Cultivator /WBS flash prep Tek /Bulk Substrate Coir Tek /Shoebox Tek /Bottle Tek /Clean spawn Checklist /Agar Recipe /Don't put Spores to Grain /Updated Pf Tek /Surface Conditions


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Re: AGAR ENVY! (Anything and All things agar!) [Re: altford78]
    #28384964 -

altford78 said:
:ohnowaitokay:

But why???? I actually believe you, but why would they not grow on 45min+ cooked media. :shrug:

Like what could possibly inhibity growth, it's just carbs one way or another.


dude investigate these two things you will get the answer.

Overheating:
Prolonged exposure to high temperatures, such as 45 minutes of pressure cooking, can potentially denature or destroy essential nutrients, growth factors, or other components present in the agar medium. This can create an unfavorable environment for mycelial growth and inhibit its development.

Nutrient Loss:
Extended pressure cooking can lead to excessive evaporation of water from the agar medium, resulting in nutrient loss and altered agar composition. Lack of adequate nutrients can hinder mycelial growth and development.


also better follow that agar cook time chart and temperatures.

i have used only PDA agar

The melting point of starch, which is a major component of potatoes, is typically around 200 to 212 degrees Celsius (392 to 414 degrees Fahrenheit).

if you over cook PDA nothing will grow on it, potatoes starch don't need to melt to lose nutrient they become useless because nutrients are loss. turns out starch is not the only thing mycelium need, some sugars are gone in potatoes as well to compensate for sugar loss we add dextrose.

The melting point of dextrose is approximately 146 to 150 degrees Celsius (295 to 302 degrees Fahrenheit).

you don't need to hit this temperatures holding 115c to 120c or 239 248 for long period of time will destroy it

the one of the reason autoclave and pressure cook operate on time and charts exists its very reason NOT OT OVER COOK IT

Edited by pesa (07/04/23 04:46 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pesa]
    #28384976 -

lolwut you think we are denaturing our nutes in the old boy presto that can barely do 25psi before blowing up. :notamused:

talk about fucking up your factor analysis


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: altford78]
    #28384983 -

yes

dude i got 13 psi and i don't go over 25 minutes. Borosilicate Glass melting point is  820 to 860 degrees Celsius (1508 to 1580 degrees Fahrenheit) but that does not mean you can stick glass petri-dish in oven.

same goes for pressure cooker, you have to follow the time rule.:shrug:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pesa]
    #28384987 -

what time rule


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] * 1
    #28384990 -

Stipe-n Cap said:
I am 100% confident that your cycle time is irrelevant. If you could produce a gelling agent impervious to microbial activity, you'll probably win the Nobel peize for your engineering achievement.

The only folks with this dead plate problem are noobs. Not one TC has ever experienced this issue, so far as I'm aware.

I  challenge you to go outside and find some mold, place it on this dead agar, report back.


Challenge accepted



Now we wait

Hope it grows mold, I really do.

The whole point of it all is that I followed a recipe exactly, high quality ingredients + distilled water, cooked it for 40 minutes, poured, etc. Before wasting time doing transfers to non-viable agar dishes, I tested one by exposing it to very dirty air for 30 seconds, 4 days later I did it again and added a dab of spit (I know), waited another 4 days still nothing. Now that plate is the same that the mold was added to. If I can't purposefully contam a plate I can't use it to determine my sterile technique or the quality of my cultures, even if it DID decide to actually grow out some mycelium. This is my concern, and ultimately the issue i am attempting to troubleshoot


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𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

Edited by Tri (07/04/23 05:06 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pesa]
    #28384991 -

I have always sterilized agar for 45 mins at around 18psi. The sterilization charts for agar can exceed 45 mins @ 15psi, don't be ridiculous.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: altford78]
    #28384992 -

agar pressure cook time is 25 minutes you can not go beyond that or it will be over cooked agar.
same goes for grains 90 minutes or 120 minutes go beyond that you will end up with burnt grain.

i guess stipe-n cap has that chart thing with agar times on it.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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