Home | Community | Message Board


Vaposhop
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Paul Stamets, Petri Dish

Jump to first unread post. Pages: 1
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
incubate agar plates
    #3634113 - 01/16/05 05:00 AM (11 years, 10 months ago)

ey!

since i been messing around with agar recently (and it all works REAL good)
i have been wondering if one could actually speed up the extremely
slow colonisation rate of peroxide agar by incubating them.

i know, that on peroxide agar the growth usually is slower,
but since i store mine at quite chilly room temps they are growing
at ridiculously slow speed.

now, i was wondering if i could simply put them into the incubator
where there's a nice 27.5C/81.5F all the time to somewhat speed
up the slow colonisation.

i understand that myc really likes this temp for growing in general,
so it should also like it on agar right?

hope for input :smile:
peace ohm :mushroom2:

*edit*
i forgot to add that i hit the search quite a bit and didnt find
the answer to my question.
i think this should also be covered in the AGAR section of the
mushroom cultivation archive.


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Edited by ohmatic (01/16/05 05:01 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: incubate agar plates [Re: ohmatic]
    #3634243 - 01/16/05 06:59 AM (11 years, 10 months ago)

allright since there was no reply i spent the last hour searching and
finally found a reference to my question.

Quote:

Incubation

It is now necessary to let the spores incubate. Place the agar filled petri dishes that have been inoculated in a dark, warm (86 F) place for a week. After a week check on them, they should show signs of growth. Over the next week or two you will start to see white mycelium forming and growing out from the center of the dish. During this phase you will probably notice that there are different types of mycelium growing. Some may seem strandy (rhizomorphic) and some may seem cottony (tomentose). The characteristic of different types of mycelium growing on the same dish is called Sectoring, this is normal and expected. Keep an eye on the dishes and look for mycelium that looks especially fast growing and that is very rhizomorphic (strandy, rope like), this is the mycelium that we want. You do not want to let the mycelium reach the sides of the dishes, as the sides of the dishes are prime spots for contaminants to be lurking. Once the dish is 3/4 colonized it is time to go onto the next step.




source: http://www.shroomery.org/index/par/8006

so ill move them to the inc :smile:
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: incubate agar plates [Re: ohmatic]
    #3634251 - 01/16/05 07:06 AM (11 years, 10 months ago)

ARGH ! after digging the mushroom cultivator,
i came across something else!

on page 37 he goes about incubating the slants at 24C,
but i guess incubating them around 27.5 cannot be that bad can it?
just wanna do the best to my little cultures,
not wanting to grill any of my babies :wink:
INPUT !!!
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 9 days
Re: incubate agar plates [Re: ohmatic]
    #3634259 - 01/16/05 07:11 AM (11 years, 10 months ago)

Every  higher  temperatures will cause a profound sweating of the agar.
I keep my agar at room temperature, I am not that in a hurry :wink:


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: incubate agar plates [Re: Anno]
    #3634269 - 01/16/05 07:18 AM (11 years, 10 months ago)

Quote:

Anno said:
Every  higher  temperatures will cause a profound sweating of the agar.
I keep my agar at room temperature, I am not that in a hurry :wink:




*mumbling strange words as he goes down to remove the plates from the incubator
he just placed them in couple minutes ago*

heh thx for the input.
if one is just about to store the petri in the inc till its about 90%
would that also be a mayor facor to water content loss or should
that be ok?
like remove it after that point and store at roomtemp.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 9 days
Re: incubate agar plates [Re: ohmatic]
    #3634272 - 01/16/05 07:19 AM (11 years, 10 months ago)

I have no idea.
Try it.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: incubate agar plates [Re: Anno]
    #3634417 - 01/16/05 09:52 AM (11 years, 10 months ago)

llright i decided to simply store them at roomtemp one remains in
the incubator for testing.
should i turn them upside down? i remember reading that somewhere.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHamurabi
the babylonianleader..

Registered: 03/31/02
Posts: 2,416
Loc: Greece
Last seen: 4 years, 5 months
Re: incubate agar plates [Re: ohmatic]
    #3634489 - 01/16/05 11:20 AM (11 years, 10 months ago)

yes the people usually turn them upside down,it's good if there is concedation.
i did yesterday my first agar work. i put most of my petri dishes in my incubator upside down


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: incubate agar plates [Re: Hamurabi]
    #3634498 - 01/16/05 11:27 AM (11 years, 10 months ago)

Quote:

Hamurabi said:
yes the people usually turn them upside down,it's good if there is concedation.
i did yesterday my first agar work. i put most of my petri dishes in my incubator upside down




nice, lets flip em over :smile:
did you use peroxide agar for ur first try? i did - with great success.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepshawny
Mycobian
Male

Folding@home Statistics
Registered: 09/11/04
Posts: 1,332
Loc: Shroomery
Last seen: 7 years, 9 months
Re: incubate agar plates [Re: ohmatic]
    #3634548 - 01/16/05 12:02 PM (11 years, 10 months ago)

You can get petri dishes with vents that help with condensation on the agar.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHamurabi
the babylonianleader..

Registered: 03/31/02
Posts: 2,416
Loc: Greece
Last seen: 4 years, 5 months
Re: incubate agar plates [Re: pshawny]
    #3634980 - 01/16/05 02:59 PM (11 years, 10 months ago)

well no i didnt use H202,i though that it's my first time,i shouldn't do it complicated!!
Even though as i know h2o2 is needed when u r gonne inoculate with a fruitbody, i did only with multispore solution.
How much h202 did u put in each petri? and i guess u did it after sterilization(or else u have killed the h2o2)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: incubate agar plates [Re: Hamurabi]
    #3635002 - 01/16/05 03:09 PM (11 years, 10 months ago)

i measured out 100ml prior to sterilisation in a filtered small jar,
let it cool till 'holdable' then injected 1ml 30% h2o2, stirred and poured.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHamurabi
the babylonianleader..

Registered: 03/31/02
Posts: 2,416
Loc: Greece
Last seen: 4 years, 5 months
Re: incubate agar plates [Re: ohmatic]
    #3635118 - 01/16/05 03:47 PM (11 years, 10 months ago)

cool. so did u put a fruitbody in these petri dishes? let me know,PM me or something when u 'll see growth to let me know how it goes:-)
good luck my friend:-)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: incubate agar plates [Re: Hamurabi]
    #3635122 - 01/16/05 03:50 PM (11 years, 10 months ago)

oh they are doing good.
click the link in my sig saying 'beer agar project' for info on
how i prepared the agar, since i modified the original recipy :smile:
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHamurabi
the babylonianleader..

Registered: 03/31/02
Posts: 2,416
Loc: Greece
Last seen: 4 years, 5 months
Re: incubate agar plates [Re: ohmatic]
    #3635170 - 01/16/05 04:06 PM (11 years, 10 months ago)

ok i will check now your project
one question:
when u say 1ml 30% H2O2.... you mean 1ml from a 30%h2o2/70% water mix?

my own question seems foolish to me! lol. maybe i drunk too much for tonight :crazy:


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinekingboomer
smurfhouse archetect
Male User Gallery

Registered: 06/15/04
Posts: 673
Loc: Rocky Mountain High!!!!
Last seen: 5 months, 26 days
Re: incubate agar plates [Re: Hamurabi]
    #3635177 - 01/16/05 04:12 PM (11 years, 10 months ago)

:mushroom2:  As long as you don't go over 90deg it should work fine.  :mushroom


--------------------
Hole in the sky/
take me to heaven..
window in time/
through it I'll fly...

-Black Sabbath


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Paul Stamets, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Whats with my agar plates? Frankenstein 2,099 6 04/08/02 05:13 PM
by Seuss
* Agar plates and gas exchange.. musher_420 936 3 03/22/07 01:44 AM
by musher_420
* What the heck is an agar plate? ShaqAttack 900 8 06/25/06 03:19 AM
by Anno
* Re: mold in agar plate Anonymous 2,023 3 12/04/99 02:35 AM
by Anonymous
* Does Agar Plate Cubensis Myc. Bruise Blue if you press on it? YoYoMadMan 660 1 12/10/08 09:53 PM
by Damion5050
* agar plates??? aful 991 7 05/09/08 09:49 AM
by aful
* Agar Plates Akira 1,082 18 01/13/06 01:17 PM
by shymanta
* What to do with 1st agar plate fromthemoon 857 3 09/07/06 05:20 PM
by monstermitch

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
2,059 topic views. 25 members, 75 guests and 25 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
MRCA Tyroler Gluckspilze
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.064 seconds spending 0.003 seconds on 14 queries.