Stipe-n Cap said:

The only isolating you're doing here is isolation from contamination. I know that this forum finds this topic contentious, for some reason, but you're not isolating individual strains or "genetics" by making plate transfers.

Symmetrical growth is an indication of health, it takes as long as it takes. The number of transfers to achieve can vary.

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I was under the impression that MSS allow different combinations of dikaryotic mycelium to form during germination.

A single drop of spores has the potential of forming millions of monokaryotic mycelia which can then mix & match to then later form different fruits.
Physically sectoring off the mycelium should cause separation of those combinations over time…
Unless you are saying every single spore will mate/germination in an orgy of spore-to-mycelium baby making and be forever bonded..?

Chamo said:

Afternoon, stipe.

“Mushroom researchers and cultivators commonly isolate mushroom strains using three methods: tissue isolation, spore isolation, and substrate mycelium isolation. Tissue isolation involves isolating a small portion of the inner tissue of fruit bodies for re-growth to obtain strains of interest, and has been employed for the isolation of the basidiomycetes, G. lucidum [7], Tricholoma matsutake [8], and Pleurotus spp. [9]. Spore isolation is mainly used to obtain single-spore cultures, which can be employed as units for hybridization breeding of mushroom species, including the successful breeding of improved Pleurotus ostreatus and L. edodes strains [10, 11]. Single-spore cultures can also be used for mating system analysis of mushrooms [12, 13]. As for substrate mycelium isolation, strains can be obtained from a substrate in which mushrooms have been grown previously [14]. Of the three methods, tissue isolation is practiced most frequently because of the low contamination rate, high reliability, and ease of manipulation.”

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Yahra said:

What should i do with this gnat infested 3 litre container in which i buried a PF cake 16 days ago?







My heart says keep it, my mind says toss it.

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