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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bagger] * 1
    #28069572 -

Who else restricts agar nutrition to develop wispier mycelium? I find it much easier to recognize anything suspicious.

I've been using GWA lately, (from WBS steep, will be testing oat boil water next), and I'm really digging it. I like to have some control plates from each batch and test how easy that particular batch contaminates. I usually don't even bother PCing them, just pour and expose it to open air maybe even touch it.

I've found that bacteria doesn't spread very fast on the GWA I've been using. I see some colonies here and there, but they have been staying small. Even molds aren't racing across it, I would say they have been colonizing at around the same speed as most mushroom species.

I don't know if that's due to limited nutrients, or if it has to do with using GW.

Thoughts, anyone?

Edit: So it turns out the GWA didn't treat me as well as I had thought. I think bacteria was hiding out but not expressing itself, because none of my spawn is clean, and I felt pretty confident about my SAB technique.

P.S. Anyone ever start an ugly agar plate thread? We could have an ugly agar party!

Edited by ruawakeyet (12/05/22 08:28 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ruawakeyet] * 3
    #28069588 -

I would guess it is the limited nutrition slowing the mold. Pretty sure you will find your growth and morphology entirely change when you move to boiled grain water, depending on the concentration you use it at

but I dunno if that which you have been using up until now is something I would desire personally

growth that is too fast to be true is a good indicator of something fucky going on, and if everything is slowed to the same speed then the two become much harder to distinguish, for me and my shitty eye at least :shrug: agar with good nute strength, molds will charge ahead immediately and it is painfully obvious

I surely have some ugly plates around somewhere... :rockon:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ruawakeyet] * 1
    #28069947 -

ruawakeyet said:
Who else restricts agar nutrition to develop wispier mycelium? I find it much easier to recognize anything suspicious.



Isn't that the theory behind water agar (using water, agar, and nothing else in a Petri dish)? Someone has some a few posts up, second post here:
https://www.shroomery.org/forums/showflat.php/Number/28068125#28068125

What's GWA?

Thanks.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28069959 -

nektar61 said:
ruawakeyet said:
Who else restricts agar nutrition to develop wispier mycelium? I find it much easier to recognize anything suspicious.



Isn't that the theory behind water agar (using water, agar, and nothing else in a Petri dish)? Someone has some a few posts up, second post here:
https://www.shroomery.org/forums/showflat.php/Number/28068125#28068125

What's GWA?

Thanks.


Not really: WA seems to speed up myc growth, assumed it's looking for nutes. WA don't do you any good to outgrow molds - only bacteria

Here -> Let's talk about Water Agar!!


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 4
    #28070035 -

nektar61 said:
What's GWA?

Thanks.


I was assuming Grain Water Agar.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 5
    #28070657 -

nektar61 said:
ruawakeyet said:
Who else restricts agar nutrition to develop wispier mycelium? I find it much easier to recognize anything suspicious.



Isn't that the theory behind water agar (using water, agar, and nothing else in a Petri dish)? Someone has some a few posts up, second post here:
https://www.shroomery.org/forums/showflat.php/Number/28068125#28068125

What's GWA?

Thanks.


Grains with Attitude...

:gangsta:


--------------------


Mushroom Cultivation Techniques...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grogu] * 2
    #28070759 -



This is the culture that had overlay
                                                Natness



That was their puck in the bottom right corner.


Edited by cozmyc (11/27/22 10:57 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cozmyc] * 3
    #28071319 -


Some nice APE mycelium, very tomentose


quick question:


These 3 are from the same culture and still had good growth speed but they have a very slight yellow mycelium near the middle, do you think its a bit of bacterias so I shouldnt use these?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 3
    #28071629 -

Some beautiful T2's...





:cookiemonster:


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Mushroom Cultivation Techniques...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28072264 -

Would that be normal looking myc for button mushrooms?

What's happening at 7h30?



--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096] * 1
    #28072298 -

Rusty2096 said:
Would that be normal looking myc for button mushrooms?

What's happening at 7h30?





looks on par. Are you going to see this one through? Agaricus bisporus kicked my butt years back. I couldn't get the casing right as it needed Bacteria to trigger the pinning. Unless some one figured out a trick/hack!

https://files.shroomery.org/files/19-34/673242238-20190825_100647.jpg


EDIT: here is a better pic scroll down https://extension.psu.edu/seeding-substrate-and-management-of-growing-agaricus-bisporus

Edited by murderlabz (11/27/22 09:42 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: murderlabz] * 7
    #28072948 -


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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD] * 5
    #28072957 -

DERRAYLD said:



Looks like the mandelbrot...



:excellent:


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Mushroom Cultivation Techniques...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grogu] * 5
    #28073456 -



Omni, Enigma type mutation off Melmak TP, in slants.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28075038 -

Seems I went backwardsโ€ฆ. I am not sure if itโ€™s the agar or the myc?  T3 looked uniform and strong.  My goal is to get a clone for each of these back to agar and then make LC.

Whatโ€™s the best path for these - send any of these to grain, scrap any, transfer again?



--------------------
๐Ÿ…ƒ๐Ÿ„ด๐Ÿ„ฐ๐Ÿ„ผ  ๐Ÿ„ฒ๐Ÿ„ป๐Ÿ„ธ๐Ÿ„ฝ๐Ÿ„ถ๐Ÿ…†๐Ÿ…๐Ÿ„ฐ๐Ÿ„ฟ

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28075070 -

It's quite common to grow from clone before making LC because you don't know how your clone culture will perform and if it'll have kept the attributes you originality were looking for until you grow it.


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096]
    #28075276 -

I havenโ€™t grown a shroom yet that has been worth cloning.  I am trying to keep my agar plates clean and take each to grain as many times as I have to in order to get a good clone candidate.  It seems T4 is showing signs that something was off in that transfer.

I am not sure if any of these can cleanly go to grainโ€ฆ


--------------------
๐Ÿ…ƒ๐Ÿ„ด๐Ÿ„ฐ๐Ÿ„ผ  ๐Ÿ„ฒ๐Ÿ„ป๐Ÿ„ธ๐Ÿ„ฝ๐Ÿ„ถ๐Ÿ…†๐Ÿ…๐Ÿ„ฐ๐Ÿ„ฟ

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD] * 2
    #28075291 -

DERRAYLD said:



Mandelbrot set!
:havesomescience:
Edit: Didnโ€™t see that someone already replied the exact same.


--------------------

Edited by San Pedro Girl (11/29/22 10:06 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas]
    #28075349 -

Bobgas said:
Seems I went backwardsโ€ฆ. I am not sure if itโ€™s the agar or the myc?  T3 looked uniform and strong.  My goal is to get a clone for each of these back to agar and then make LC.

Whatโ€™s the best path for these - send any of these to grain, scrap any, transfer again?





Bottom left B+ ,
Bottom left & top right APEU I would send to grains

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Whyterye]
    #28075644 -

Anyone get tiny water spots on the surface of their agar sometimes? Like the size of a pinhead and completely invisible except when looked at the surface with a light. I canโ€™t even get a good pic.

Iโ€™ve never found them to be a contaminant and distilled water has cut down on seeing them for me. My other theory is that itโ€™s condensation dripping down from the lid and then drying on the surface.

Just wondering if others see this often?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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