Nichrome said:

Rusty2096 said:

Hey guys,

These 2 jars are bacterial but they don't look so bad to me (the condensation in the right one was there since I took it out the PC, it's not from bacteria heat)



My plan is just to top fruit straight in the jars to take prints. Do you all think they would recover from a shake or should I not try my luck?

Thanks

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I wouldn't diagnose those as bacterial. I have a high standard though for only calling actual bacteria bacteria, and I have and use a microscope daily. I see no slime, or pooling or anything I'd call bacteria. I do see overly wet grain and burst grains which can slow and alter growth patterns due to the moisture and the starches converted to sugars which now cover all the grains. Naked eye evaluation of microorganisms is pretty weak in general unless it's green or obvious. Mycelium can look a lot of different ways.

Personally I'd shake them. They'll likely be fine.

Did that grain ferment a bit before it went in the pc? A lot of the time the leftover enzymes and metabolites from bacteria and other microbes before pc can be mycotoxic after the pc causing slow, weird, or no growth at all. Looks like a bacterial problem without the bacteria when that happens.

"Bacterial" is one of the main go to old wives tales to explain what is not understood by the cultivator/community yet. It's just one of those things that gets repeated.

(Please feel free to share any bacteria cultures with me that exhibit swarm motility, especially in extreme environments.)

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uninc4life2010 said:

Okay.  What is a good option if I want to cool the tent?  Is there any way to chill the water in the fogger?

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Quote:

I wouldn't diagnose those as bacterial. I have a high standard though for only calling actual bacteria bacteria, and I have and use a microscope daily. I see no slime, or pooling or anything I'd call bacteria. I do see overly wet grain and burst grains which can slow and alter growth patterns due to the moisture and the starches converted to sugars which now cover all the grains. Naked eye evaluation of microorganisms is pretty weak in general unless it's green or obvious. Mycelium can look a lot of different ways.

Personally I'd shake them. They'll likely be fine.

Did that grain ferment a bit before it went in the pc? A lot of the time the leftover enzymes and metabolites from bacteria and other microbes before pc can be mycotoxic after the pc causing slow, weird, or no growth at all. Looks like a bacterial problem without the bacteria when that happens.

"Bacterial" is one of the main go to old wives tales to explain what is not understood by the cultivator/community yet. It's just one of those things that gets repeated.

(Please feel free to share any bacteria cultures with me that exhibit swarm motility, especially in extreme environments.)

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Well, I did underline that they were bacterial for a reason: these jars were inoculated with the same LC used on this plates (noct 3 but only have a picture of that one, they all looked the same):



I am almost done cleaning it on agar and soon new jars will be inoculated using clean agar soon.



I appreciate your opinion and expertise, and hope you are right, somehow (that LC was so nasty, just flaming the needle smelled like burning sugar). Maybe the myc "killed" the bacteria in the jar. I don't know if that's even a thing. On some older agar T1s and T2s the bacteria stopped growing, and became darker - straight up looks like it's dying.

I shook the jars 10 hours ago so for now I keep my fingers crossed.

canadashroomdude said:

hi!

Three questions:

Do I pick this big guy soon?




Does this look too dry to you?



Any ideas as to why I'm not getting any in the middle!?

Okay, maybe 4 - do these look okay to you? Most PE pics I see, don't have the small white dots on the caps.

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Zigzagzin said:

What is the best way / technic to dry sclortia?

Also when spawning shoeboxes is it better to cover the shoebox in foil and poke holes or just use the lid. I've seen it done both ways and wanted to know if it made a difference or if it was just preference.

Thanks

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Kibby said:

Can you inject water into a cake using a syringe rather than soaking it?

Wouldn't that cut down 24 hours of time to a few seconds?

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HILLBILLY OUTLAW said:

I usually see growth in about 3 days.
15-20 for full colonization.

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Smellyhobbit said:

HILLBILLY OUTLAW said:

I usually see growth in about 3 days.
15-20 for full colonization.

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Really? I usually see full colonization with just A2G in a little under 2 weeks. Might not be worth the extra step.

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shroomsurf said:

Hi, I'm watching video's and studying how to use a PC. I'll probably use whole ry berries and heard somewhere the time to PC in jars differs from the unicorn bags.

On the site I'll grab some rye berries it says 90 minutes at 15 PSI for jars, so how much longer does it have to PC with unicorn bags.

They also say to soak for 12-24 minutes then cook for 15-20 minutes...

https://mushroommediaonline.com/mushroom-substrates/Whole-Rye-Berries-p183543846

I was just going to get bags, then saw they had grain too...

Also when wrapping the bags, which will be 4qt for me, do I wrap the filter inside or outside?

Thanks,

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LtLurker said:

Smellyhobbit said:

HILLBILLY OUTLAW said:

I usually see growth in about 3 days.
15-20 for full colonization.

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Really? I usually see full colonization with just A2G in a little under 2 weeks. Might not be worth the extra step.

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Gonna depend alot on how heavy or light you pour it. If you pour enough that atleast 25% is colonized right after germinating, then you're skipping right to 1st shake. 2 weeks a2g sounds a little quick to me, usually takes me a week just to really get it going from the wedge.

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rumfor69 said:

This should help

https://www.shroomery.org/forums/remlinker.php?Cat=&Entry=285542&F_Board=2&Thread=27163702&Main=27163702

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Thanks,