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Re: Post Your Cultivation Picture of The Day [Re: saralove]
    #27865935 -

I think I’ll buy some wide mouth half pints and run a wire through some caps. Toss one in the PC when I run out while I’m doing other stuff, then print on it when I need to. Seems like you could just put 40ml of distilled water in there, agitate with a marble, and boom you got 4 syringes.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Extras: Filter Print Post Top
Re: Post Your Cultivation Picture of The Day [Re: bigfootscreepyuncl]
    #27865939 -

bigfootscreepyuncl said:
Smellyhobbit said:
Has anyone tried printing into a jar and then adding distilled water to make syringes?




Distilled water is NOT sterile. If I were to experiment with that method I would use distilled water but it must still be sterilized. AFAIK when people make spore syringes they'll fill a syringe with distilled water, wrap it in foil, sterilize the whole thing, then shoot the sterile water onto the print, scrape the spores with the needle then suck the solution back up



I see people suck up boiling water into sterile syringes and squirt it into a clean dish. That’s what I did, anyway.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Post Your Cultivation Picture of The Day [Re: saralove]
    #27865940 -

MysticMycologist said:
Beachbum251 said:
MysticMycologist said:
The middle cake, yes. They are ready. They look good.

Why are the fruits so wet? Asking for a bad case of blotch, IMO



Misted this am. Wasn't sure if ready so I wanted to keep doing like I had been until someone from here helped. Thanks again and down the hatch!



Next time you should check out the water tub fruiting chamber. In my experience, it is much more effective at holding the correct humidity for cubensis cakes, especially if your SGFC is not packed full of cakes. This will eliminate or greatly reduce the amount of misting needed, which makes things much easier.

Good job though, those are some pretty mushrooms, and welcome to the Shroomery!


@Rotnpins
I have never tried printing onto agar, and I feel like it would make the resulting germ plate almost unmanageable, but it is cool. When I do first prints, I do keep them for myself. I kind of like cleaning up dirty prints on agar. It is good practice for working with field prints from outdoor finds. I reserve my “cleaner” prints for giveaways and such, as I often never know the recipients intent or agar skill level, and always want to send the best I can. Please do a giveaway. Giveaways are one of the most wholesome parts of this community, and I feel like the mushrooms want us to spread them far and wide. It is the Lord’s work. Keep up the good work Rotn!




For sure.. I've been trying to do frequent giveaways.. I figure, for what you spend on 1 order from a vendor, you can get a box of envelopes, a box of swabs, a roll of foil, and a roll of stamps.. it doesn't make sense not to do it :lol:

I'll have some kick ass giveaways coming up pretty soon... I've got jars of Phobos, Choco TAT, FP+, and Tidal Wave going right now. I've got a full roll of stamps, so hopefully I'll be able to send something other than GT and APE soon :rockon:

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Re: Post Your Cultivation Picture of The Day [Re: bigfootscreepyuncl] * 1
    #27865957 -

Smellyhobbit said:
Has anyone tried printing into a jar and then adding distilled water to make syringes?



I do spore collection jars. 1/4 pint wide
mouth, make a wire trivet stand, grain jar
lid setup, PC for 20mins.

Place cap on wire stand, put the lid on,
wait till print has dropped, remove cap and
wire stand.

There'll be condensation in the jar, it will
clear in a few days, then you can just
vacuum seal the jar, and store in the fridge

I'd just use BODs easy caveman sterile syringe
water method, squirt in the jar, stir, draw
it up.


--------------------

Links For Beginners And Beyond:mspoil:

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Re: Post Your Cultivation Picture of The Day [Re: rumfor69]
    #27865959 -

Going to look into that when I get home. Would love to do my in house prints this way.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Extras: Filter Print Post Top
Re: Post Your Cultivation Picture of The Day [Re: Smellyhobbit] * 1
    #27865967 -

Syringes are good if ur gonna do cakes.
I use agar so I just buy individual wrapped
sterile swabs, swab the print in the jar,
Dab/streak plate


--------------------

Links For Beginners And Beyond:mspoil:

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Re: Post Your Cultivation Picture of The Day [Re: rumfor69]
    #27865979 -

rumfor69 said:
Syringes are good if ur gonna do cakes.
I use agar so I just buy individual wrapped
sterile swabs, swab the print in the jar,
Dab/streak plate



I’m doing cakes until I figure out the problematic variable with my agar. Plus I’m trying to get certain friends of mine growing as well, so having a syringe to throw at them for their first cakes that they don’t have to buy is helpful.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Post Your Cultivation Picture of The Day [Re: sandman420] * 1
    #27866031 -

sandman420 said:
What makes you think they are monokaryons? Do you have some way of dealing with single spore or just serial dilution?

Not doubting you or anything I just know very little about this aspect.





These I verified as being monokaryotic with my compound microscope. The cells lack clamp connections which is indicative of how many nuclei are in the cell at that time. And yes, I do both. I have my own technique that I developed for isolating monokaryons and I certainly do a good bit of serial dilution as well.


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Re: Post Your Cultivation Picture of The Day [Re: Judge Doom] * 4
    #27866041 -

Gonna make some gummies for the first time.

I’m not sure how potent you can get tea so might be a little bit of a waste, but this was 50g Melmak.




--------------------
LAGM2020

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Re: Post Your Cultivation Picture of The Day [Re: A.k.a]
    #27866050 -

Which gummy recipe are you going to use, and how big of a batch are you going to do? My last batch of gummies came out tasting great but lacked potency, although I only used 30g of standard cubes. I have like 8oz I need to either cook with or compost - I'd like to make it so that each bear (I make gummy cactus because i already had the silicone ice cube moulds on hand) is ~1.75g


--------------------



I :heart: 5318008


NOT a virgin!

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Re: Post Your Cultivation Picture of The Day [Re: bigfootscreepyuncl] * 1
    #27866057 -

I’m trying to make them as potent as possible.

Normally for weed I make coconut oil then add that to half a cup of water and I think 85g jello. I’m reducing the tea right now and just gonna try it with the same method.

Originally I was going to use 100g but figured I should do a test batch first. It’s kind of a project as a storage method for somebody else who can’t have loads of mushrooms sitting around.


--------------------
LAGM2020

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Re: Post Your Cultivation Picture of The Day [Re: A.k.a] * 1
    #27866077 -

A.k.a said:
Gonna make some gummies for the first time.

I’m not sure how potent you can get tea so might be a little bit of a waste, but this was 50g Melmak.






I make my tea this way, then I freeze it for when I want it. Using just distilled water and fresh fruits I can potentially fit 100g worth of alkaloids into 1 fluid ounce of water. Usually I keep it around 50g per fluid ounce personally but it is possible. Using reduction it's quite easy to get down to such a small volume, it just takes careful concentration the smaller you go so you don't overdo it. If you end up reducing it too much in some cases it is possible to just pour some more water in but if it gets too far its too late. I've never tried gummies though, definitely thought about it..


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Re: Post Your Cultivation Picture of The Day [Re: A.k.a]
    #27866086 -

A.k.a said:





Oo she dark. This is what I Wana do in time.


--------------------
You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Re: Post Your Cultivation Picture of The Day [Re: cozmyc] * 1
    #27866146 -

First flush of B+ featuring some fuzzy feet. Currently working on more FAE but coming along well otherwise. Running another tub that I started a couple weeks after this one using standard polyfill in the holes, whereas with this one I tried out filter disks. I think I’ll stick with polyfill.


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Re: Post Your Cultivation Picture of The Day [Re: MysticMycologist] * 3
    #27866195 -

MysticMycologist said:
I think Fahtster does something similar using a jar, a wire holder, and all inside of an oven bag. I can’t remember his exact process, but  he is well know for having the cleanest prints and swabs around, despite his unconventional techniques. In my limited experience working with Faht’s samples, they may be the only spores I would ever consider taking straight to grain. I’m paranoid, so I wouldn’t, but fern plates from his giveaways have produced the cleanest germ plates I have ever made.

(Forgive the fallatio, Faht. :blowme: I can’t help myself.)



Haha.  Well I appreciate the kind words. 

You don’t have to use the complicated oven bag set up.. I just do a lot of spores to grain (very small amounts of grain so the loss, if any, is also small) work and eliminating as many contamination vectors as possible is the goal there but lots of ppl do great with SAB’s.  Pf use to do the jar print set up when he sold his syringes so it’s nothing new but I guess my contribution to the idea would the use of magnets to scrape the spores into the sterile water.


Here’s the whole thread, but like I said, you can simplify it much more with a SAB

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Re: Post Your Cultivation Picture of The Day [Re: fahtster] * 3
    #27866201 -

Quote:





You should send that bolt to grain :grin:

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Re: Post Your Cultivation Picture of The Day [Re: Vibetyme]
    #27866231 -

Vibetyme said:
Rotnpins said:
hummingbird said:
My first APE tub...



I was thinking the dark caps were aborts, but most of them still seem to be growing...



Check out this thread



I thought the same thing.  They look exactly like the starry night apes I've seen




I was thinking this too, hopefully they keep growing...those starry nights are probably the coolest looking cubes I've ever seen...we'll see what happens I guess.

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Re: Post Your Cultivation Picture of The Day [Re: A.k.a] * 2
    #27866246 -

A.k.a said:
Gonna make some gummies for the first time.

I’m not sure how potent you can get tea so might be a little bit of a waste, but this was 50g Melmak.






Fresh or dry?  I can get tea down to half the weight of the fresh caps through boiling them in their own juice with 2% citric acid and pressing thoroughly after steeping.  Thinking about making agar gummies from that for mini-dosing rather than doing dried fruits.


Nat's 3rd flush this morning, ready (and slightly beyond ready - should've picked the big ones on the left yesterday, but lazy :lol:):



Been taking clones from these more interesting phenos coming off my original mixed lineage on the left above.  My first try at getting wavy cap pheno from G1 of a print of one of the first desired caps is the tray on the left - none there at all.  I've got more of those to try though.

But this is kind of an interesting Nat color variant, gonna clone it:



--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 

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Re: Post Your Cultivation Picture of The Day [Re: A.k.a] * 1
    #27866295 -

A.k.a said:
Gonna make some gummies for the first time.

I’m not sure how potent you can get tea so might be a little bit of a waste, but this was 50g Melmak.






You can cook teas down pretty far, just be sure to reduce the heat and stir, silicone spatula is your friend. I made a jar last night too, 42.8g pan cyan 'Alabama' and 1g bisporus. It is about a 1/4 pint and around 1/2 is honey. :smile:


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Re: Post Your Cultivation Picture of The Day [Re: YoshiTrainer] * 4
    #27866331 -

That’s a lot of pans :lol:


It was 50g dry. Cooked down to like 3/4 cup probably and made gummies.


This little guy should be about half a gram so we’ll see how it did.




Definitely not nearly what I expected. Light Boiling isn’t supposed to affect potency right? Gotta be either that or I didn’t squeeze the shroom bits hard enough.


--------------------
LAGM2020

Edited by A.k.a (07/17/22 03:15 PM)

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Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   Myyco.com APE Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag


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