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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD] * 4
    #27858581 -

DERRAYLD said:
Never wipe with iso after flaming.
You either accept the soot or you get a flame that doesn't cause soot.


It's also down to blade material.

Carbon steel soots heavily, stainless steel does not, at all.

Smellyhobbit said:
I’ve been wiping with iso after flaming because I’m worried the tool is too hot and will damage mycelium and spores.

Is this a needless worry?



This is why you've been having problems in your plates. Swab a plate with ISO alcohol and see what a mess you get.

ISO your handle, then flame your blade.


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Being pissed on does not make you a real man.

...OR DOES IT?

Edited by Guerrilla (07/11/22 11:49 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla] * 1
    #27858700 -

you can always sandwhich with cold agar. Just cut a square and lift it on the sample. I've done it. The agar will grow through to the top AND out the sides


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420]
    #27858754 -

sandman420 said:
you can always sandwhich with cold agar. Just cut a square and lift it on the sample. I've done it. The agar will grow through to the top AND out the sides



Cool. Thanks guys. I'll probably try both pucks and the cold sandwich and see which way I like best. Hopefully I'll be able to clean up a few of the cultures that have given me issues


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Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 1
    #27858793 -

I’ve done the cold sandwich with good results, tho you may want to use a thick piece for the top, you’ll want to make a shallow cut to avoid transferring whatevers growing below….

I don’t have those kinds of skills to cut that shallow, so I ended up placing a third tiny piece on top of that for 1 day then transferred that piece away

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Re: AGAR ENVY! (Anything and All things agar!) [Re: san pedro guy] * 1
    #27858794 -

ive done the sandwich too but i like the brf puck/poke because of the versatility.

you can go from dirty culture to colonized lc really fast using the same tools.

multitaskers give me a hard on.


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #27858801 -

mushboy said:
clean yo shit
:josex:


Oh sweet, great tek to know. I was thinking about making a batch of antibiotic agar, but this seems like a solid approach.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Seamonkey84] * 2
    #27858851 -

i aint rich antibiotic agar is extra money inflation is real ill stick with cheap grain powder, tools, and simple techniques:snub:


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy] * 1
    #27858889 -

mushboy said:
i aint rich antibiotic agar is extra money inflation is real ill stick with cheap grain powder, tools, and simple techniques:snub:



Fish antibiotic called Furan-2 by Yapi contains Nitrofuran. Supposedly that survives PCing. I've never used it but is on eBay for 39 bucks:
https://www.ebay.com/itm/324791460187

I've seen it cheaper.


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--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #27858907 -

mushboy said:
i aint rich antibiotic agar is extra money inflation is real ill stick with cheap grain powder, tools, and simple techniques:snub:


I already have doxycycline on hand, but I’d need to get a IR thermometer to know when it’s ok to mix in the antibiotic and pour.

Reading over that PF plate tek, I noticed he says to use a small 21ga needle. I only have a box of 16ga, I got the bigger needles since I’m mostly working with LC and they clog easier 😅. I found it somewhat hard to get a piece of agar to stick in the needle doing the poke when making LC. But according to the clean up tek, the goal isn’t to grab a piece like a mini core sample. He says just to get a few cells stick to the inside of the needle, good thing I caught that. Only other needles I have are insulin syringes, I wonder if 30ga is too small lol.

Edited by Seamonkey84 (07/11/22 08:14 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Seamonkey84] * 1
    #27859580 -

larger needles take more of a biopsy poke like a core sample. you are correct the idea is just to have some myc fragments stick to the needle.

smaller the better  ...not what she said:awesome:


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27859601 -

The BRF cleanup tek is very interesting. I’m wondering what a good use case would be for it when genetic material is usually so abundant in later game hobby mycology.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #27859602 -

Smellyhobbit said:
The BRF cleanup tek is very interesting. I’m wondering what a good use case would be for it when genetic material is usually so abundant in later game hobby mycology.


Wild prints and clones :shrug:

Or a culture you really want but can never seem to get a clean sample


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27859612 -

Regardless, it’s really interesting how that substrate seems to handle bacteria! I’m glad for it. My PF jars seem to be thriving.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 2
    #27859958 -

All y'all transferring plate pins and me over here transferring teeth.

Lions mane


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc]
    #27859986 -

I just got two plates going of a lions mane I cloned from the grocery store. One was a needle biopsy squirt onto the plate, another was three pieces I cut out. Both seem to be growing nothing but mycelium so far, the one done with a poke has more growth it seems. The biopsy piece is growing along with some scattered colonies that are all growing the same as the larger piece. I’ll take a pic when I get home.

Edit: can’t get a picture, glass jar no pour agar and condensation on the sides. But the growth all looks consistent with lions mane mycelium, and the poke plate definitely has more growth. The satellites all appear to be dislodged cells from squirting from the syringe, they’re growing faster than the solid pieces.

Also, I’ve heard to pick the pieces off as they continue to want to fruit, then take transfer from the active growth once it stops throwing fruit.

Edited by Seamonkey84 (07/12/22 06:50 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Seamonkey84] * 1
    #27860058 -

TAM PG & APE SN



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Re: AGAR ENVY! (Anything and All things agar!) [Re: labbar]
    #27860243 -

@labbar noice plates bro those look ready for grain

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27860312 -

I made a batch of 50 condiment cups about 3 weeks ago.. I checked them after 10 or 11 days and they were clean.. now I'm down to the last 10 and 6 of them look like they have 1 tiny speck each that's bacterial :shrug: isn't bacteria usually a lot faster than this? The plates have been wrapped and sealed in a ziplock the whole time.


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27860575 -

A while back, I'd posted some of my agar pictures that were quite questionable:



I let those grow out a more, and to be honest they still looked pretty wispy. I've transferred the leading edges, and a few look a bit better, but nothing like what I see from some other awesome pics here.

At the same time, I took some wedges to grain just based on a feeling, and... it went from that, to what looks like a good start to growth?!



At this point, I'm wondering if there's something off about my agar recipe or ingredients. Maybe it's not solid enough and the mycelium is embedding in the top layer and looking translucent (maybe the liquid drop from MSS contributed). Not sure!

But so far, these jars are looking as I'd expect them to. Time will tell, I guess.

In the meantime, I'm gonna get my next set of plates going on a slightly different agar concoction. Slightly lower agar/LME, maybe some nutritional yeast.

Edited by rcraft (07/12/22 11:17 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 1
    #27860586 -

Rotnpins said:
I made a batch of 50 condiment cups about 3 weeks ago.. I checked them after 10 or 11 days and they were clean.. now I'm down to the last 10 and 6 of them look like they have 1 tiny speck each that's bacterial :shrug: isn't bacteria usually a lot faster than this? The plates have been wrapped and sealed in a ziplock the whole time.


I had a similar experience with unused condiment cup plates left to sit undisturbed for about 15 days, checked on em and they had a perfectly clean appearance. Moved to a different shelf, and within 3 days when I came back, a good number of them had a tiny bacterial blob show up. Only theory I could come up with after that much time, was that it must’ve been knocked onto the surface from an inside wall or from the lid while switching shelves.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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