nektar61 said:

Techgrower77 said:

Mike Hawck said:

Techgrower77 said:

So I'm pretty new to all of this.

I transferred some TED spores to agar and not sure if I should be concerned about the yellow?

Thanks!

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Good candidate to put to some pasty-plates filled with PF mix.

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So, I'm sorry but I don't have any idea what you are talking about lol

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My sigline has a link to the Advanced Search and a link to some (important) suggestions for using it.

I can't tell if the Pasty Plates comment is joking or not. But Pasty-plates are using containers from the grocery store instead of petri dishes.

Personally, I would throw out these plates, the yellow is serious contam. There is no way in hell those put on grain will make good spawn. The yellow will out-compete the shroom mycelium.

There are people here with more experience than me who would absolutely try (and succeed) to transfer a sliver of the good mycelium out of there to a new plate. But if you're very new, it might be too much.

I can't tell if it's mold or bacteria, but with bacteria I'll try to transfer out the good mycelium to a new plate, but with mold, I usually don't, it can fill your house with mold spores. I just leave them sealed and throw them out.

If the contam comes up a couple days after inoculation, it's often bacteria. If a week or so, it's often mold. This looks kinda like bacteria, but I don't know.

Your mileage may vary.

I recommend pouring plates, taping them up, and waiting a week or ten days before using to see if they're contammed on their own.

Here's an excellent post by a guy who really knows his stuff, all about making transfers (and making agar before that, but it seems you've made some).
https://www.shroomery.org/forums/showflat.php/Number/26628234

EDIT: I think Mike was joking, since someone who knows their shit replied with a "WTF?" image to his comment here:
https://www.shroomery.org/forums/showflat.php/Number/27552455#27552455

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nektar61 said:

Techgrower77 said:

Mike Hawck said:

Techgrower77 said:

So I'm pretty new to all of this.

I transferred some TED spores to agar and not sure if I should be concerned about the yellow?

Thanks!

---

Good candidate to put to some pasty-plates filled with PF mix.

---

So, I'm sorry but I don't have any idea what you are talking about lol

---

My sigline has a link to the Advanced Search and a link to some (important) suggestions for using it.

I can't tell if the Pasty Plates comment is joking or not. But Pasty-plates are using containers from the grocery store instead of petri dishes.

Personally, I would throw out these plates, the yellow is serious contam. There is no way in hell those put on grain will make good spawn. The yellow will out-compete the shroom mycelium.

There are people here with more experience than me who would absolutely try (and succeed) to transfer a sliver of the good mycelium out of there to a new plate. But if you're very new, it might be too much.

I can't tell if it's mold or bacteria, but with bacteria I'll try to transfer out the good mycelium to a new plate, but with mold, I usually don't, it can fill your house with mold spores. I just leave them sealed and throw them out.

If the contam comes up a couple days after inoculation, it's often bacteria. If a week or so, it's often mold. This looks kinda like bacteria, but I don't know.

Your mileage may vary.

I recommend pouring plates, taping them up, and waiting a week or ten days before using to see if they're contammed on their own.

Here's an excellent post by a guy who really knows his stuff, all about making transfers (and making agar before that, but it seems you've made some).
https://www.shroomery.org/forums/showflat.php/Number/26628234

EDIT: I think Mike was joking, since someone who knows their shit replied with a "WTF?" image to his comment here:
https://www.shroomery.org/forums/showflat.php/Number/27552455#27552455

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Techgrower77 said:

I do have more of the spore syringe, but I am wondering if the syringe it's self is contaminated, those plates had been sealed and sitting for several weeks and were clean (I tossed a few that were contaminated) at the exact same time I had another syringe in the stab and inoculated 5 of 2 different strains (maybe not the best idea) but the other 5 are clean, but all 5 of the same syringe have the yellow contamination.

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Quote:

Techgrower77 said:.

I do have more of the spore syringe, but I am wondering if the syringe it's self is contaminated, those plates had been sealed and sitting for several weeks and were clean (I tossed a few that were contaminated) at the exact same time I had another syringe in the stab and inoculated 5 of 2 different strains (maybe not the best idea) but the other 5 are clean, but all 5 of the same syringe have the yellow contamination.

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D3_Myc said:

Giving yeti another go to replenish swabs for giveaways

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D3_Myc said:

Giving yeti another go to replenish swabs for giveaways

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Plz DM me when you have the giveaway

nektar61 said:

Techgrower77 said:

I do have more of the spore syringe, but I am wondering if the syringe it's self is contaminated, those plates had been sealed and sitting for several weeks and were clean (I tossed a few that were contaminated) at the exact same time I had another syringe in the stab and inoculated 5 of 2 different strains (maybe not the best idea) but the other 5 are clean, but all 5 of the same syringe have the yellow contamination.

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I didn't think you were joking, I thought Mike was joking.

There's no such thing as a sterile syringe, regardless of marketing.

I'd put it to grain first, using a good, simple grain tek....PCing - pressure cooking - grain in jars for 2 hours, plus time for altitude, keep jar loose during PCing.

Wait until the grain jar is cool and inject a small amount, like 1 CC of spore syringe solution into it, inside an SAB, then grow some shrooms and clone them. Clone the fattest, fastest growing shroom.

I recommend shaking up syringes well, the spores are so tiny you can't see them. Some sellers add charcole so you think there are a lot of spores. Shake the fuck out of them before use, or shake by touching for 60 seconds against a vibrator, or blender, before use.

Or do the ancient tek of PF tek. Then clone the fattest, fastest growing shroom.

Or get enough posts on here to get some free spores on here, and put those to agar. Or buy some spores and put them to agar.

Let us know if you have any questions after looking up this stuff and reading about it.

Spore syringes put to agar often are contaminated.

Basically grow some shrooms, make some prints, clone them, etc. First thing is grow some shrooms, even if not many and they're not perfect. Then you have more genetic material to work with.

Good to have you here.

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Telling somebody to put a spore syringe to grain is a big no no. It’s like rule #1 of the shroomery. Keep spore syringes away from grains period!

Clean up on agar or go PF tek.

Tormato said:

D3_Myc said:

Giving yeti another go to replenish swabs for giveaways

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Plz DM me when you have the giveaway

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Guerrilla said:

Telling somebody to put a spore syringe to grain is a big no no. It’s like rule #1 of the shroomery. Keep spore syringes away from grains period!

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Guerrilla said:

Clean up on agar or go PF tek.

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