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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Camera93]
    #27366165 -

YES this is contaminated to hell, but (I think) it’s still pretty damn sexy!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Camera93]
    #27366586 -

Camera93 said:
Transferring to a sterile jar of grain may result in some pretty quick colonization
Crons got a post on it somewhere


I see. Interesting- the myc was pretty speedy.

I have the very first clone plate which is the mother in the fridge along with the invitro pin dish grown out. i currently have 4 transfers out of that invitro plate- im doing too much lol

Edited by moshe1 (06/28/21 10:19 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1]
    #27366730 -

What do you guys think of mycelium that bruises blue in its older age on agar? I notice it on many of my older plates, especially PE ones, but read somewhere that Bod thinks that any bruising is indicative of bacteria caused stress. Any insights on this?

Edited by multifractal (06/28/21 10:13 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27366749 -

I think it’s sometimes from drying out as they get really old.


He could be right about bacteria though, I’ve had some that never bruised.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #27366750 -

I always assumed bruising. I see it more on strains that bruise if you look at them wrong.


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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27366756 -

multifractal said:
What do you guys think of mycelium that bruises blue in its older age on agar? I notice it on many of my older plates, especially PE ones, but read somewhere that Bod thinks that any bruising is indicative of bacteria caused stress. Any insights on this?


Some are more temperature sensitive in my experience. They will blue from being cold. Drying out will cause it as well. I have some strains that turn blue once I put them in the fridge within a few days.


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Eclipse3130]
    #27367683 -

Quick question about germination plates. This is 4 days off a third spore print. Does anyone see any red flags? I've been unsuccessful with 2 prints already.



Thanks in advance


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.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Atomsplit]
    #27367687 -

Nope looks like a clean germ plate :thumbup:


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Atomsplit]
    #27367733 -

Atomsplit said:
Quick question about germination plates. This is 4 days off a third spore print. Does anyone see any red flags? I've been unsuccessful with 2 prints already.



Thanks in advance


I can see plenty to work with... take small samples and you should be good... :thumbup:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27367738 -

Going back to the bruising, this article popped up in my feed today

https://psychedelicreview.com/comparing-psilocybin-mushroom-bruising-with-trichoderma-infection/


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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #27367889 -

A.k.a said:
Benson said:
D3monic said:
Benson, what you using for your backdrop? I tried a piece of black acrylic and it’s too reflective




Just my black desk! You can see the grain on the left. 1/80s f/4.5 800iso indirect daylight. Beautiful plate as always, they look like little trees :mushroom2:



I think black felt is what they use.



Black velvet is great.


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27368170 -

T3 of Maz
10g agar
9g LME



How am I doing?


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BHO's Ultimate List of Lists
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Re: AGAR ENVY! (Anything and All things agar!) [Re: B H O]
    #27368243 -

Beautifully. Well done.


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2021 Outdoor Hydroponic Garden
The Hyphal Knot and Rhizo Transfer Pictorial
LAGM2021
Brown Bear's  Grow Log Adventure
SET IT & FORGET IT GROW TENT
CornCobTek
BagPrep
MyBestCanopies
The hardest thing in the world is for a warrior to let others be.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: B H O]
    #27368247 -

nice B H O, i love Maz... it's like my highschool sweetheart but in cultivation lol.  Myc lookin thicc


over 20 sleeves of dishes later... my career first invitro fruit popped out today from my own germination/ untested cultures.
On a transfer plate that started out 27 days ago-  excited to test this dish.




reaching for the stars



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Edited by moshe1 (06/29/21 10:47 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1]
    #27368288 -

Just for fun, i am going to make some agar using mushroom tea as the liquid base. probably will use slightly less BRF to compensate for the nutrients available from the mushrooms or would these be insignificant? I will probably use 2 grams of dried mushrooms per 250 ml water and ~6 grams of BRF. Alternatively, I might use the tea with a small batch of germination agar prepared using cornmeal and karo.

Edited by multifractal (06/29/21 11:31 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27368484 -

multifractal said:
What do you guys think of mycelium that bruises blue in its older age on agar? I notice it on many of my older plates, especially PE ones, but read somewhere that Bod thinks that any bruising is indicative of bacteria caused stress. Any insights on this?


I only ever find agar bruising on plates that are old and drying out.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27368645 -

A T1 APE plate from MSS, 21 days old. Should I be excited about this? Ive noticed a color change where the water is beading up. Water droplets are stacked ontop each other. 


--------------------
Check your vector

Gummies by milkboy
Milkboys Public Display of Affection
I'm a Semp


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Re: AGAR ENVY! (Anything and All things agar!) [Re: milkboy]
    #27368657 -

milkboy said:
A T1 APE plate from MSS, 21 days old. Should I be excited about this? Ive noticed a color change where the water is beading up. Water droplets are stacked ontop each other. 



Do you store your plates upside down? When I was starting out (month and half ago) and I couldn’t get my pour temps right, I was getting lots of condensation and storing the plates upside down help me keep the beading up water off the agar. Even having the condensation issue under control I still store my plates upside down.


--------------------
BHO's Ultimate List of Lists
Print Library

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Re: AGAR ENVY! (Anything and All things agar!) [Re: B H O]
    #27368665 -

I dont store my plates upside-down at this stage. There wasnt any condensation in the container, I was under the impression the mycelium was knotting up to form pins...


--------------------
Check your vector

Gummies by milkboy
Milkboys Public Display of Affection
I'm a Semp


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Re: AGAR ENVY! (Anything and All things agar!) [Re: B H O]
    #27368668 -

B H O said:
I couldn’t get my pour temps right, I was getting lots of condensation and storing the plates upside down help me keep the beading up water off the agar. Even having the condensation issue under control I still store my plates upside down.


If you store plates upside-down for any lengthy period of time they will dry out, so just be aware of that. I found this out the hard way years ago.

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Shop: Original Sensible Seeds High THC Strains   Mushroom-Hut Mono Tub Substrate   North Spore Injection Grain Bag   Myyco.com APE Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy

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