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Josex
#cheat_code

Registered: 11/13/15
Posts: 8,999
Loc:

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27364535 - 06/26/21 11:01 PM (5 years, 4 months ago)

What are those plates with scre-on lids? Something like holy grails?

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Josex]

1
#27364540 - 06/26/21 11:06 PM (5 years, 4 months ago)

Josex said:

What are those plates with scre-on lids? Something like holy grails?

Yea, the lil bead containers, thought I’d give them a try. Lil less waste hopefully. Use them for swab dunks and t1’s maybe. A lil small though, be nice if I could find something more like 90mm

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moshe1

Registered: 08/29/15
Posts: 378
Last seen: 4 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: BrownBear]
#27364541 - 06/26/21 11:06 PM (5 years, 4 months ago)

impeccable ropes. what do you use for your black color or charcoal?

We know that rhizo growth is more-or-less the growing form myc shifts over to when it needs to cover ground for whatever reason. I remember reading since back when how important it was in order to select the myc that grows the quickest, and it seemed it was accepted as a crucial step in the cultivation process.

When im in discussion or reading around now i notice that the sentiments are preferential, although most probably still go for it.

so after the hobby has evolved for however many years now, why did we go from thinking about it as a paramount step in the process to more of a preference thing? What did we learn?

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Edited by moshe1 (06/26/21 11:11 PM)

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Benson
The Kidd ⭐

Registered: 09/29/20
Posts: 898

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27364580 - 06/26/21 11:42 PM (5 years, 4 months ago)

D3monic said:

Benson, what you using for your backdrop? I tried a piece of black acrylic and it’s too reflective

Just my black desk! You can see the grain on the left. 1/80s f/4.5 800iso indirect daylight. Beautiful plate as always, they look like little trees :mushroom2:

:mushroom2:

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SpaceBaby
alchemist

Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 3 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: BrownBear]
#27364823 - 06/27/21 05:56 AM (5 years, 4 months ago)

Iambrownbear said:

All three look good but that T1 treasure coast is beautiful.

:fuckyeah:

Thank you, BB. Practice, practice practice. I have 3 media botles empty atm, and two more are on their way. To my horror there isn’t a plate left in the house that is not growing out. Time to try out my heated mag stir plate. :dancer:

:dancer:

Man I hate standing at the microwave.

Agar Pic Tax. A plate from 06.22:

Clone tissue from a beautiful, big [for me] Malabar fruit. The Malabar and B+ ar currently producing the most fruits.

I’ll UTFSE, but meanwhile, anybody got a swab TEK they especially like?

Edited by SpaceBaby (06/27/21 06:00 AM)

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,007
Loc: South Africa
Last seen: 22 minutes, 16 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27364834 - 06/27/21 06:35 AM (5 years, 4 months ago)

D3monic said:

Benson, what you using for your backdrop? I tried a piece of black acrylic and it’s too reflective

Go for a solid color and edit it out, green screen.

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rockyfungus
dirty

Registered: 03/01/21
Posts: 1,097
Loc: Front range
Last seen: 21 days, 19 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27364854 - 06/27/21 07:39 AM (5 years, 4 months ago)

D3monic said:

Josex said:

What are those plates with scre-on lids? Something like holy grails?

Yea, the lil bead containers, thought I’d give them a try. Lil less waste hopefully. Use them for swab dunks and t1’s maybe. A lil small though, be nice if I could find something more like 90mm

After using them for about 2 years it's hard to see into my bead containers. Combination of scalpeling the plastic, and some deformation from PC cycles? The clarity fades, but the reusability makes it so worth it. Slap a piece of tape on a lid or container side and you got a label that lasts a few PC cycles. Only problem is my parafilm has been just sitting for years.

Cold storage of my beads, lions mane is the only one you can see and the rest are oysters, enoki, shiitake.

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King87
Stranger

Registered: 06/04/16
Posts: 60
Last seen: 3 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: King87]
#27364887 - 06/27/21 08:36 AM (5 years, 4 months ago)

King87 said:

Trying out yeast for the first time to try and get me away from slow growth and more toward rhizo.

Update;

Cant believe what a difference adding yeast has made to the quality of the myc growing. After nearly a year of getting really slow growth and zero rhizo, all I added was 1g yeast to my normal mix and within a couple of days the plates are looking strong with rhizo growth, just thought I'd say in case it helps anyone else.

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,225
Loc: Gaming the system
Last seen: 2 hours, 53 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Benson]
#27364900 - 06/27/21 08:59 AM (5 years, 4 months ago)

Benson said:

D3monic said:

Benson, what you using for your backdrop? I tried a piece of black acrylic and it’s too reflective

Just my black desk! You can see the grain on the left. 1/80s f/4.5 800iso indirect daylight. Beautiful plate as always, they look like little trees

I think black felt is what they use.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27365027 - 06/27/21 12:02 PM (5 years, 4 months ago)

A.k.a said:

Benson said:

D3monic said:

Benson, what you using for your backdrop? I tried a piece of black acrylic and it’s too reflective

Just my black desk! You can see the grain on the left. 1/80s f/4.5 800iso indirect daylight. Beautiful plate as always, they look like little trees

I think black felt is what they use.

I was hoping for something I could wipe down with iso and have open cultures on in front the hood. Maybe I’ll just give it a coat of matte black. Looked into that black 3.0 but apparently it’s really chalky and delicate

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B H O

Registered: 03/17/13
Posts: 675
Last seen: 2 years, 7 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27365665 - 06/27/21 11:49 PM (5 years, 4 months ago)

Worked on some T3 transfers of some Maz today.

I made 4 hole punch transfers and for the final transfer I used an inoculation loop to pick up a single rope of rhizo myc and transferred it.

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Big_Dub
I'm just some guy

Registered: 01/12/11
Posts: 3,649
Loc: Los Angeles

Last seen: 1 day, 10 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: B H O]
#27365675 - 06/27/21 11:56 PM (5 years, 4 months ago)

I like the loop idea but keep forgetting to try.

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B H O

Registered: 03/17/13
Posts: 675
Last seen: 2 years, 7 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Big_Dub]
#27365676 - 06/27/21 11:57 PM (5 years, 4 months ago)

Big_Dub said:

I like the loop idea but keep forgetting to try.

People kept telling it was gonna be a game changer and they weren’t wrong lol

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Atomsplit

Registered: 01/17/21
Posts: 1,578
Loc: SAB

Re: AGAR ENVY! (Anything and All things agar!)

[Re: B H O]
#27365686 - 06/28/21 12:26 AM (5 years, 4 months ago)

B H O said:

Big_Dub said:

I like the loop idea but keep forgetting to try.

People kept telling it was gonna be a game changer and they weren’t wrong lol

So you use a loop to pick off a strand?
Please explain.

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Big_Dub
I'm just some guy

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Posts: 3,649
Loc: Los Angeles

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Atomsplit]
#27365692 - 06/28/21 12:32 AM (5 years, 4 months ago)

Ya.

You don't need an inoculation loop. You just need a way to grab a piece of myc, which are small as hell. Consider Josex poke. More or less the same idea

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nix21
Stranger

Registered: 05/29/18
Posts: 624

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Big_Dub]
#27365780 - 06/28/21 03:42 AM (5 years, 4 months ago)

This is my T1 APE plate from swab, where would you guys take transfer for T2.. i m looking anywhere between 12 and 6, is good?

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moshe1

Registered: 08/29/15
Posts: 378
Last seen: 4 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: nix21]
#27365802 - 06/28/21 04:36 AM (5 years, 4 months ago)

random ? here as usual,

are there any benefit from transferring an invitro pin that grew on the dish from a monotub pin clone?

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Rafiikii

Registered: 11/17/10
Posts: 2,892

Re: AGAR ENVY! (Anything and All things agar!)

[Re: nix21]
#27365817 - 06/28/21 05:27 AM (5 years, 4 months ago)

nix21 said:

This is my T1 APE plate from swab, where would you guys take transfer for T2.. i m looking anywhere between 12 and 6, is good?

Personally I would take from 12 to 2. But honestly there’s plenty more to work from then just that

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nix21
Stranger

Registered: 05/29/18
Posts: 624

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Rafiikii]
#27365821 - 06/28/21 05:49 AM (5 years, 4 months ago)

Since it looks pretty clean maybe i should let it ride for few more days till some good sector come up?

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Camera93
We got dicks like Jesus

Registered: 08/15/18
Posts: 3,589
Last seen: 1 month, 19 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: moshe1]
#27365859 - 06/28/21 07:23 AM (5 years, 4 months ago)

moshe1 said:

random ? here as usual,

are there any benefit from transferring an invitro pin that grew on the dish from a monotub pin clone?

Transferring to a sterile jar of grain may result in some pretty quick colonization
Crons got a post on it somewhere

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