Churchill_85 said:

One question regarding SAB, the correct is to mist the SAB first then put everything inside right? Just took a look and another unused poured plate had mold, so im 70 - 80% sterile...



OBS: SAB used to be an fruiting chamber, i closed those with plastic tape =]

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I quit spraying the inside. I simply wipe out with iso sloppily before setting the SAB down  (I move my sab around from use to storage.) Iso hands.
Place work inside.
Iso hands then get to work.

Question! Do you keep your lid on the tote and place your work on that? (Hard to tell if the lid is on or not, sorry)

If so that could cause a problem maybe? I noticed setting my work on the lid caused the lid to compress and or snap back to it's original memory causing a disturbance of air within the -not so- still air box now. Throwing contams around with agar is no good

Hopefully this helps someone else out if anything!

senorpoto said:

Thanks for the reply. I'm not sure what soft agar is - I just followed the regular agar tek (Bhod's) using agar powder & malt extract.  It's worked well for cloning and other spore growing.

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