tryptkaloids said:

Typically it is.

The way the rhizos seem seperate make it seem like the myc is trying to outrun something

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coversall said:

I made a small batch of no-pour agar today, 6g agar, 5g dried potato flakes, 250ml water, a drop of karo, and some food colouring.

The potato flakes wouldn't totally disolve and so there is a layer of sediment in the bottom on my dishes. Is this normal and ok?

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Hiya...I'm still a noob but I've done a number of runs with PastyPlates (awesome) and have just shifted to plates.

I usually use a PDA/PHA mix using Idahoan Potato Flakes and sediment is kinda normal for me.

Don't have many PastyPlate pics to illustrate but here's a backlit one of a transfer I did to a plate of PHA mix recently. You can see the myc, but the sediment is floating there in the background.



Hope this is helpful.

Enjoy!

WFG

A.k.a said:

Thick ass natalensis clone myc

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Holy crap that's beefy...is it always like that for that variety?

WFG

WunFunGai said:

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jay.ach said:

WunFunGai said:

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Great looking plates!  How hard was the jump from Pasty's?

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Hey thanks!

I'm still a noob, but I went to Pasty's with my first attempts at cloning.

You can go straight to dishes, and I see a lot of experienced folks recommend it, but Pasty Plates were a good agar starting point for me.

I've done probably 40-60 plates in the past using either the round frosty glad cups, or the squarish and more clear ZipLoc ones. They worked great and didn't seem as "formal" as plates.

With that said, if you've done Pasty's before and are comfortable with that, it's not a hard leap at all to go to plates. In fact, once you've done it, and see how much more you can see and observe, you'll wonder why you didn't do it sooner.

I'd strongly encourage you to try it at least once. You can get 30 90x15mm plates off amazon for $14 and a media bottle for $12.

Link to plates that I just got and will be trying soon: here
Link to media bottle (got 2) that work great: here

Big thumbs up! Many knowledgeable and informative posts in the forums.

I've been logging my first try at isolation and plates in another thread and plates have really been nice for being able to really observe in detail what's going on down there.

I still have my Pasty containers and I'm thinking I'll use those to make wedges to go to grain with, since they are reusable and if I'm propagating from a known plate, I won't be as concerned with observing the fine details as much as getting a puck to drop onto grain quickly and easily...that will be my first chance to log some Tiger Drop action

Hope this info was helpful.

Enjoy.

WFG

P.S. Oh heck, I'm all fired up and gonna make some agar now!

WunFunGai said:

Hey Doc! I wonder if it has something to do with the color? Shucks I forgot which thread but I recall reading where some red dyes were made using bugs...anti-fungal bugs.

If that were the case, that might explain the inhibited growth on that color, and normal on others.

I usually stick to my favorite colors of blue or green or mix them a bit if I'm feeling spicy...lol

WFG

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kingleonidas said:

Shit, that's what I was afraid of. Any ideas why the myc isn't taking? My recipe was 10g agar 7g LME and 500ml water. Do I need some yeast?

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You don't need yeast. I use pretty much the same recipe. I do 8g LME instead of 7g. I haven't had any plates make it to grain yet. Theres a few that are starting to take off pretty decent but the rest I keep having to transfer away from mold or bacteria.

I transferred this one as soon as mold started near the edge and kept it around to see what it would eventually turn into. Its getting pretty gnarly.


I thought for sure that this one was gonna be my winner but sure enough, there's mold germinating near the edge again. I cut two small pieces out of the rhizo edge and put them on their own plate. Maybe one of them will stay clean.



Here's a plate that I intentionally contaminated to see what it would do. Lots of pretty colors.


It's always exciting to come back to a plate after 15 hours and see how its changed.

Rafiikii said:

circular wedges?

please explain




looks great btw

dig the agar color too, really pops the rhizos

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