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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Re: morel cultivation thread [Re: 7Suns]
    #27497430 -

That's so strange, I wonder if it is freak genetics, like the guy who got chaga to fruit on agar and is the only one I've ever seen do it?

Or was there a specific agar recipe?


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
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Re: morel cultivation thread [Re: Forrester]
    #27498386 -

He mentioned it on Reddit it’s a special agar recipe not genetics


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Re: morel cultivation thread [Re: 7Suns]
    #27498639 -



I found his post on Reddit.

The agar is regular PDA with blue dye:
https://www.reddit.com/r/mycology/comments/p4nrcx/comment/h900zp0/

Do you think it's special blue dye??

Edited by dr-mephesto (10/09/21 02:37 PM)

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Re: morel cultivation thread [Re: 7Suns]
    #27499073 -

7Suns said:
it’s a special agar recipe not genetics


      :thumbup:

      ...in combination with specific environmental parameters.


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Re: morel cultivation thread [Re: Forrester]
    #27499099 -

Forrester said:
I wonder if it is freak genetics, like the guy who got chaga to fruit on agar and is the only one I've ever seen do it?




      I think it was the birch sap.

      https://www.shroomery.org/forums/showflat.php/Number/26464628#26464628


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Edited by Sporulator (10/10/21 04:12 AM)

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Re: morel cultivation thread [Re: Sporulator]
    #27499105 -

Sporulator said:
      I think it was the birch sap.

      https://www.shroomery.org/forums/showflat.php/Number/26464628#26464628


Hey that's cool I've never seen that one.  I was referring to when Amanita Virosa did it.  I don't think he used anything special in the agar as he wasn't even attempting to fruit it.  That's what makes me think genetics, maybe in both cases...


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.

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Re: morel cultivation thread [Re: Forrester] * 1
    #27499450 -

Pda is critical because of specific enzymatic processes not every fungi possess the ability to to eat every form of sugar

Morels utilize sucrose, cellulose, and starch through sucrase, cellulase, and amylase

Understanding the appropriate pathways of each fungi’s specific catabolic process tells us what media to start them on

Also nitrogen is a key element for the fungi to produce these digestive enzymes

But I agree with sporulator it is environmental and nutrition which has been speculated for years

Genetics are only a very small fraction of the equation here and in my honest opinion not responsible for his agar plate fruiting

My guess is he had it in cold storage and upon removing it to appropriate temperatures it triggered it

Do some research on sclerotinia sclerotiorum it is almost parallel to the fruiting process described in the patents

I’ve got a specific formula for a spawn medium I came up with for morels and it has been amazing it’s completely consumed and becomes an entire stone

I will release the recipe once I get fruit, I just spawned stones to trays of coconut coir and now I’m running a continuous sprayer for 24 hours I’ve had them in cold storage for 3 months which I believe is a key element (why they only fruit in spring they need cold storage 40f and under) I limed the coco coir with slack lime / instant lime to a ph of 8
I didn’t start spraying until Constantinella cristata (conidia/powdery mildew) formed on the coir surface and I’ve been rotating temperature  day and night between 50-60f for the past week and will return it to this cycle after watering so fingers crossed I’m at the final stages of this and might get pins, last time I got primordial balls on the mycelium but it stalled








If you look back at my previous posts you will see the difference between morels on straight grain (failed no stones)
Vs. half grain half potting soil (some stones but not of appreciable size)
Vs. new recipe (entire media becomes a large stone) 👌

Anyways I stand by my statement pins in agar is one thing but I won’t applaud until I see it on substrate and enough fruit bodies to be considered for commercial or practical purposes

I’m to invested to give up now 😂


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Edited by 7Suns (10/10/21 03:46 PM)

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Re: morel cultivation thread [Re: 7Suns]
    #27499474 -

Please post the recipe. At least others can try working with large sclerotia now. The more people working on it the better.

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Re: morel cultivation thread [Re: dr-mephesto]
    #27499525 -

When I get fruit bodies I’d be more then willing to do a tek /write up for the community I’m not here to get rich


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Re: morel cultivation thread [Re: 7Suns]
    #27505580 -

NICE!!!!!👍👍👍👍👍
Now I ask myself: How does that sclerotia know when it's SAFE to vegetate, or so UNSAFE that it needs to fruit?😉

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Re: morel cultivation thread [Re: Pezizales]
    #27505685 -

Current status of the sclerotia I just spawned, we have micro pins forming between the substrate and the container !!








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Re: morel cultivation thread [Re: 7Suns] * 1
    #27521842 -

Updates.... the pins died because substrate dried too quickly, I’m about to drop new spawn in a tray and try again with a “loamy” substrate to help hold moisture better and this time will also be putting them in the fog chamber with my oysters and other gourmets to encourage the pins to keep going, from what I’ve come to understand the primordia take about 2 weeks to a month to mature after initial appearance

Also discovered something interesting when I did a ph test on the sclerotial mass I cultured in jars on my special spawn mix,  it is slightly acidic before inoculation between 5-6 ph (very aggressive and nearly consumed the whole spawn mixture)

after several months of incubation the morel mycelium has actually basified the spawn and it’s own tissue to about 8-9 ph at which point it seems to have “stalled”

To me this disproves the theory that they create acids as they digest their food, this simply isn’t true!!!
they actually produce mild to strong alkaline substances!!
This has prompted me to try an experiment on my next batch of spawn where I play with different ph ranges to see what it prefers

this makes me wonder if we’ve had it wrong this whole time, the mycelium might actually need slightly more acidic soil to help it digest its substrate and only needs alkaline soils to trigger fruiting ?? Or maybe it’s just tolerant of these ranges ??




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Re: morel cultivation thread [Re: dr-mephesto]
    #27522451 -

dr-mephesto said:


The agar is regular PDA with blue dye:



      For what purpose the blue dye?


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Re: morel cultivation thread [Re: Sporulator]
    #27523997 -

:hewut:  :thumbup:
Im excited about your recipe!

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Re: morel cultivation thread [Re: mariapilz] * 1
    #27524017 -

Thank you so far it has been good to me, I hope to share it with everyone soon just waiting for mature fruit, so far I’ve only made it to pinning and then they abort but that’s still further then most


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Re: morel cultivation thread [Re: 7Suns]
    #27524306 -

How did you started this thing out? With spores from a dried fruitbody? A multispore culture?

Do you know the exact species? morchella. ..what? Maybe also the origin?


Thank you for sharing and good luck!

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Re: morel cultivation thread [Re: mariapilz]
    #27524740 -

It’s morchella angusticeps from either workman (sporeworks) or Lenny  A Rockwell  I can’t remember I think it’s the latter, but I chose this strain because it’s such a prolific stone producer,


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Re: morel cultivation thread [Re: 7Suns]
    #27525178 -

A microscopic photo would be really great ....from dish and substratsurface and.... it's not the case that I do know anything about microscopic things. But maybe other eyes:wink: Im just interested in

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Re: morel cultivation thread [Re: mariapilz]
    #27525184 -

I’m not sure I understand what you’re asking?


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Re: morel cultivation thread [Re: 7Suns]
    #27525226 -

Oh sorry. my english is terrible :rolleyes: can only get better

I was just hoping that you have a microscope and that you could possibly post microscopic photos of the mycelium. just hoped.

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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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