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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Re: morel cultivation thread [Re: catnip40] * 5
    #28725809 -

In garden covered in woodchips last Fall, southern Oregon coast. Morchella importuna. Spore water method ages ago, completely forgot about it. Not sure if these are even the same as the spores.



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Re: morel cultivation thread [Re: Workman]
    #28746081 -





Today, I went to Korea's national agricultural center to learn morel cultivation.

This center successfully cultivated morels in Korea for the first time.

I visited late season, so most of morels are picked/rot

The process I learned is same as china method(nothing special), and the strain is M.sexlanta

These are way harder to cultivate indoors


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List of artificially cultivated mushroom from entire world(over 300+ species)

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Re: morel cultivation thread [Re: lastine]
    #28746315 -

Sorry if this has already been asked but I know old apple orchards are a good place to forage for morels.

Ive been grafting small apple trees, has anyone tried soaking young rootstock in a slurry and planting after?

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Re: morel cultivation thread [Re: Parkridge]
    #28746347 -

Hey !
This spring nature gave me a nice gift, some morel grow in my garden.


I tried some biopsy and spore print to clone them and maybe start a little culture next winter.
As I am new to this species, I do not know if have suceed and got real myc or it is just some mold growing? I am aware that all that plates have contam and need some transfert but I would like to avoid the time and effort if it is not even morel myc growing haha
The third one was full of bacterial contam but look like some myc in place of where I put the tip of morel try to emerge. For the 2 others, lot of white fluffy stuff but I don't feel like it is really morel. I will try to do water agar for the next transfert. What do you think?



Edited by GRETO (04/23/24 09:02 AM)

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Re: morel cultivation thread [Re: GRETO] * 1
    #28746501 -

Greto that looks correct on all 3 plates, morel mycelium tends to start white and turn brown/orange within a few days-weeks so don’t be thrown off if it starts to look lumpy and darker colors, it’s actually a bad culture if it stays white and never develops sclerotia or turns brown/orange


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Re: morel cultivation thread [Re: 7Suns]
    #28747485 -

Thanks for the advice !
The third one is definitely well contaminated. Maybe hard to see on the photo but it is multicolor of bacterial contam
I will let them sit and see if I see some coloration
If I don't get any I can do assume that it is not morel?

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Re: morel cultivation thread [Re: GRETO]
    #28747553 -

Correct, the mycelium should start to brown or yellow and the agar itself will also darken from matabalization, if it stays white it’s either a different mold/fungi or a non-viable morel culture (lack of sclerotia production and color change is a sign of senescence in this species) with the exception of the half free morel and morchella diminutiva which seems to stay white and floccose

Check out the pics in the link

https://encrypted-tbn0.gstatic.com/images?q=tbn:ANd9GcQYpayqOLYPewvsIZvBrFa7ms6RWg-6Bbkyqhq65i_auw&s


https://amb-express.springeropen.com/articles/10.1186/s13568-021-01325-2


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Re: morel cultivation thread [Re: 7Suns] * 1
    #28747710 -

Oh f**, I did thrown away some others plates that looked exactly like this photo hahaha

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Re: morel cultivation thread [Re: GRETO] * 1
    #28748192 -

Yea that’s why I try to be as informative as possible lol most people see morel mycelium and trash it their first time because we’re so used to everything being perfectly white except contamination


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Re: morel cultivation thread [Re: 7Suns]
    #28748791 -

Yeah it is totally counterintuitive haha
How much time should I wait? Plate are 2-3 week.

And so it get me another question. How to well spot contam if myc is same color and strange looking?

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Re: morel cultivation thread [Re: GRETO]
    #28748960 -

If those plates are 2-3 weeks old I would wait 1 more week if they arnt browning or lumpy by then, you more then likely have a bum culture, in all honesty morel mycelium is fast and you should have already seen browning and sclerotia after 2 weeks  at least in my experience but that can also be strain dependent and agar recipes can change the look of mycelium from the same isolate

What was your starting point for genetic material? Spores or tissue samples? I would recommend spores because tissue usually has a lot other contamination feeding on the morel fruit bodies


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Re: morel cultivation thread [Re: 7Suns]
    #28749031 -

Theese 3 are tissu, I also did spore print. Here some bad phooto



I can spot some little sclerotia I think like little orange crust. But again, my plate still look contaminated. I can be wrong but some color/texture really look like bacteria/others mold

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Re: morel cultivation thread [Re: GRETO]
    #28749185 -

I think I also see some sclerotia in there yes I would try to isolate them those plates look more like morel mycelium then the other ones you posted


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Re: morel cultivation thread [Re: 7Suns]
    #28751468 -

Is it usefully to try to transfert the sclerotia or it is counterproductive and better to try to isolate myc?

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Re: morel cultivation thread [Re: GRETO]
    #28751548 -

Either will work but if you transfer actual sclerotia it should be easier to identify any contamination


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Re: morel cultivation thread [Re: 7Suns] * 2
    #28755554 -

I have a proposition for anyone who might be interested, although this isn’t the market place i figured this would probably be the best place for it, anyone that is interested is welcome to message me and discuss this in more detail

I have found a viable resource for confirmed dna sequenced morchella rufobrunea culture and not only that but it is confirmed to be the original commercially produced culture used by Gary Mills in one of his first morel growing facilities back in 1998, this is coming from PennState
College of Agricultural Sciences, the culture is $150 + $50 shipping & Handling this includes 2 100mm round Petri dishes guaranteed clean and viable

My proposal is to crowdfund the price of this culture and everyone that put in towards it will receive 2, 50ml culture slants from me upon transferring the original culture to new dishes (I recently came into possession of 500 sterile 50ml slants so there will be plenty to go around)

If anyone is interested in this please check out my journal and leave a comment with how much you want to contribute ($20 minimum buy in)






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Edited by 7Suns (05/02/24 10:23 AM)

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Re: morel cultivation thread [Re: 7Suns]
    #28757623 -

There have been many group buys in this forum for edible cultures. I took part in a mass shiitake culture group buy years ago. I recently passed my cultures along and they have been revived. I say go for it! I would probably be willing to help.

As far as the M. rufobrunnea culture I was supposed to receive (but didn't)...I reached out to Shroomery sponsor Out Grow via e-mail. I'll let you know if I ever hear back.


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:dragon:

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Re: morel cultivation thread [Re: 7Suns]
    #28757837 -

7Suns said:
I have a proposition for anyone who might be interested, although this isn’t the market place i figured this would probably be the best place for it, anyone that is interested is welcome to message me and discuss this in more detail

I have found a viable resource for confirmed dna sequenced morchella rufobrunea culture and not only that but it is confirmed to be the original commercially produced culture used by Gary Mills in one of his first morel growing facilities back in 1998, this is coming from PennState
College of Agricultural Sciences, the culture is $150 + $50 shipping & Handling this includes 2 100mm round Petri dishes guaranteed clean and viable

My proposal is to crowdfund the price of this culture and everyone that put in towards it will receive 2, 50ml culture slants from me upon transferring the original culture to new dishes (I recently came into possession of 500 sterile 50ml slants so there will be plenty to go around)

If anyone is interested in this please reach out in a private message,







Yeah I sent u a message
Definitely excited to take my hobby to the next level or theee


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Re: morel cultivation thread [Re: fnulnu] * 1
    #28758937 -

Here’s a link to a separate thread I made for the WC 833 rufobrunnea culture to avoid derailing this thread

https://www.shroomery.org/forums/showflat.php/Number/28758924/vc/1#28758924


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Re: morel cultivation thread [Re: Dragonaut] * 1
    #28762059 -

That sucks you didnt get your syringe, i got my morel americana from them during the free giveaway


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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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