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Offlineucsdmolpath
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morels and chanterelles to swap for maitake and hericum?
    #2290957 - 02/01/04 01:54 AM (17 years, 1 month ago)

hi all :smile:

i've never tried growing morels or chanterelles, but a friend of mine has requested that i do, since she loves to cook them...in anycase, i have a few extra maitake and hericum wooden spawn plugs that i could trade for morel and chanterelle spawn plugs or agar wedges....also any info on their culture would be most appreciated! 

=^_^= molpath


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Offlineragadinks
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Re: morels and chanterelles to swap for maitake and hericum? [Re: ucsdmolpath]
    #2291105 - 02/01/04 03:40 AM (17 years, 1 month ago)

When you have grown your first chanterelles please post some the tek and some pics here  :rolleyes:

No, seriously - you should do a search on that kind of topics before you post them here.


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Invisiblevampirism
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Re: morels and chanterelles to swap for maitake and hericum? [Re: ucsdmolpath]
    #2291378 - 02/01/04 09:39 AM (17 years, 1 month ago)

how experienced are you btw?
Chanterelles are.. impossible ..to grow indoors right now
morels are extreeeemely finicky, and are expensive to produce anyway..


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Offlineucsdmolpath
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Re: morels and chanterelles to swap for maitake and hericum? [Re: ]
    #2292005 - 02/01/04 04:16 PM (17 years, 1 month ago)

well, i knew morels were difficult to grow, i didn't think that they carried such a reputation...a few responses to some questions on here...

1) insofar as my experience, while i'm new to fungi cultivation, i'm a molecular biologist who deals with mammalian cells, insect and bacterial cells. considering i too have to stringently control temperature, CO2, humidity, salt and mineral molarities, serum and other facters--all in sterile conditions...so, to me, fungi are really not all that different...just a matter of finding and providing the right conditions...the basis of most experiments...

2) in any case, i posted after reading a section in stamets which states that morel mycelium colonize grain medium very fast in bottles, it's just a matter of providing the right outdoor casing which can usually work with the right balance of ash, peat moss, and some other stuff which i don't remember right now.

3) chanterelles, i've never read about and just threw them in because i had heard of them and my friend was interested....i knew that they decompose fallen leaves in their natural environment and frankly, i thought it might be cool to try growing them in a bottled media of autoclaved leaves and grain...and now i know that they are difficult to grow, kind of like how people thougth reishi were really difficult to grow so many years ago. you should also keep in mind that i never said i attempting to grow anything indoors.

truly, i did not mean to piss off or patronize anyone when writing on here, i was just politely trying to find and share information like the rest of you.


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OfflineBasidiocarp
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Re: morels and chanterelles to swap for maitake and hericum? [Re: ucsdmolpath]
    #2292097 - 02/01/04 05:23 PM (17 years, 1 month ago)

If you are a scientist, then you should appreciate the value of an exhaustive literature search before you undertake an experiment or project. Some things that are well-known:

Outdoor morel cultivation has a fair to good success rate. The stuff with ash that you're talking about is part of the actual substrate, not the casing. Outdoor morel patches a la Stamets are not cased per se. They also take at least a year to bear fruit, if you're lucky.

To my knowledge, only the yellow morel has been successfully cultivated indoors a la the patented procedure by Ower. I have heard of no reports of the black morel fruiting indoors. I tried the Ower procedure with the black morel and failed. Stamets has tried variations on the Ower procedure with the black Morel and failed.

Chanterelles are mycorrhizal fungi, which means that their mycelium engages in a mutualistic/symbiotic relationship with the roots of host plants/trees. There have been a few reports of controlled cultivation of chanterelles, but the one I heard about required the growing of tree saplings in pots and then encouraging a mycorrhizal chanterelle/sapling relationship. Yields were, to my understanding, poor. So, I don't think you'd have much success growing chanterelles on dead leaves. If you're looking to make chanterelle spawn to introduce into the outdoors, sawdust spawn would be far superior to grain or grain/leaves. Grain breaks down too quickly outdoors and attracts unwanted pests.

Despite your molecular biology experience, I would recommend getting your feet wet with some easier-to-grow-species. In doing so you will learn invaluable skills, tricks, and knowledge that will help you tackle more difficult species.


--------------------
"...if the mind is actually part of a continuum, a labyrinth that is connected not only to every other mind that exists or has existed, but to every atom, organism, and region in the vastness of space and time itself, the fact that it is able to occasionally make forays into the labyrinth and have transpersonal experiences no longer seems so strange."

Visit the Psychonautical Society


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Offlineucsdmolpath
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Re: morels and chanterelles to swap for maitake and hericum? [Re: Basidiocarp]
    #2292163 - 02/01/04 06:00 PM (17 years, 1 month ago)

thank you, dr. honeydew...

that kind of response is much more informative and productive for me than prior spats...

my point, readers may have noticed in my response to other recent replies, was that perhaps regardless of area of specialization, is that these kind of public informative forums should offer a more encouraging environment. while i may have knowledge of stringent biochemical pathways in the oncology of cancer and genomic instability which is what i research, and while i may know where to find the vast resources of specialized information on this subject, i doubt most mycologists might be so familiar. however, i would not discourage or ridicule people with limited knowledge on the subject as in previous responses. frankly, if this forum expects members to have a certain level of knowledge before joining or posting, perhaps there should be some sort of preliminary examination?

i hope that others could take dr. honeydew's response as a prime example of what a productive and informative response should be. far be it from me to offend anyone with my mycological ignorance. but are we not all ignorant beings in some regard, seeking to learn more about this world?


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Offlineesp
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Re: morels and chanterelles to swap for maitake and hericum? [Re: ucsdmolpath]
    #2292576 - 02/01/04 09:34 PM (17 years, 1 month ago)

If i any case, you would feel like trying to grow morel indoors, here is TEK you could give a try:
http://www.mushroompeople.com/cat2000/morel.html

According to the pics, I looks like a successfull TEK...

Good luck!


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OfflineBasidiocarp
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Re: morels and chanterelles to swap for maitake and hericum? [Re: esp]
    #2292694 - 02/01/04 10:42 PM (17 years, 1 month ago)

Quote:

esp said:
If i any case, you would feel like trying to grow morel indoors, here is TEK you could give a try:
http://www.mushroompeople.com/cat2000/morel.html

According to the pics, I looks like a successfull TEK...

Good luck!




Yes, this TEK is a variation on the patented procedure originally conceived of by Ower. It can work for the yellow morel, Morchella esculenta. I tried it and failed using a black morel strain Morchella augusticeps. The procedure requires lots of specific substrate materials, precise growing conditions, and is quite labor-intensive. Even if you do follow the TEK down to every detail, do not be surprised by failure: Morel strains are hypervariable with resepct to indoor fruiting ability; morel cultures senesce quickly, and will fail to produce sclerotia and mushrooms after as few as 5 - 10 petri plate subcultures; and finally the TEK/patent procedure itself has several steps where contamination and failure are possible/likely.

Morel culture is an area of ongoing research. Keep your eyes and ears tuned in...


--------------------
"...if the mind is actually part of a continuum, a labyrinth that is connected not only to every other mind that exists or has existed, but to every atom, organism, and region in the vastness of space and time itself, the fact that it is able to occasionally make forays into the labyrinth and have transpersonal experiences no longer seems so strange."

Visit the Psychonautical Society


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Offlineucsdmolpath
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Re: morels and chanterelles to swap for maitake and hericum? [Re: Basidiocarp]
    #2293048 - 02/02/04 01:57 AM (17 years, 1 month ago)

thanks for the advice....appreciate it :smile:


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Offlineragadinks
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Re: morels and chanterelles to swap for maitake and hericum? [Re: ucsdmolpath]
    #2293148 - 02/02/04 03:05 AM (17 years, 1 month ago)

Quote:

that kind of response is much more informative and productive for me than prior spats...




I think you allude to my post with your statement:
I did not want to put you off and keep you from asking questions in this forum. But it should be common practice to read the FAQ's and inform oneself roughly about a topic before posting in a forum.
If you have a look at the pinned down posts in most of the forums it just says that ( e.g: see the advanced section, or the mushroom cultivation section ). This prevents new users from asking the same questions over and over again ( and maybe boring other users ).
So, please post questions, but please just take the time and inform yourself roughly about the topic before you post it.

Hope I do not have offend you with this post ?


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Offlinezeronio
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Re: morels and chanterelles to swap for maitake and hericum? [Re: ucsdmolpath]
    #2293157 - 02/02/04 03:09 AM (17 years, 1 month ago)

Cantharellus can be cultivated - here is the tek or better the PhD thesis :grin: :
http://www.mykopat.slu.se/mycorrhiza/kantarellfiler/texter/rtf.htm
However the culture will be hard to obtain...

The only mycorrhizal culture I have is Amanita muscaria. 3 months after cloning the mycellium covered less then 1cm2 of normal PDYA agar. :crazy:


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Offlinefalcon
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Re: morels and chanterelles to swap for maitake and hericum? [Re: ucsdmolpath]
    #2295714 - 02/02/04 10:49 PM (17 years, 1 month ago)

I''ve been thinking about cloning chantrelles this summer. I don't think I would grow them on any normal media, they get their carbohydrates from host trees and i'd bet probably from mostly sugars. So I'll probably wan't a mineral rich sugar agar with some source of protein or nitrogen (maybe urea).

Once I get them growing on agar they will probably have to go onto malted grain mixed with (?) so the carbs are easier to get at for the chantrelles.

Then I'll pop them out of the wide mouth jars I grew them in and have myself chantrelle tempeh.

I don't really think its going to be this easy, but it would be nice.


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OfflineBasidiocarp
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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #2295785 - 02/02/04 11:15 PM (17 years, 1 month ago)

Quote:

falcon said:
I don't really think its going to be this easy, but it would be nice.




Nah, won't be that easy...  But you might want to consider using one of the minimal mediums that plant tissue culture people use...  Basically Glucose or sucrose plus N/P/K and some other trace minerals.  MS medium is a good place to start.  Do keep us informed of your progress.  :smile:


--------------------
"...if the mind is actually part of a continuum, a labyrinth that is connected not only to every other mind that exists or has existed, but to every atom, organism, and region in the vastness of space and time itself, the fact that it is able to occasionally make forays into the labyrinth and have transpersonal experiences no longer seems so strange."

Visit the Psychonautical Society


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Offlinefalcon
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Re: morels and chanterelles to swap for maitake and hericum? [Re: Basidiocarp]
    #2295870 - 02/02/04 11:49 PM (17 years, 1 month ago)

OK. Minimal media sounds good. :laugh:  I wont be finding any chantrelles till June though :frown:.


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #2296261 - 02/03/04 03:15 AM (17 years, 1 month ago)

In the link that zeronio provided it says that they have used following agar mediums:

Quote:

1. Murashige & Skoog medium (MS) (Table 5) was supplied with antibiotics (50 ppm streptomycin and ampicillin, 5 ppm benomyl) and then sterile filtered (0.2 um Sartorius cellulose acetate filter). MS was then mixed with an autoclaved agar solution (final agar concentration 1.2%) with 0.05% activated charcoal (to adsorb toxic compounds in autoclaved agar), before addition to 9-cm petri dishes.




Quote:

2. Modified Fries medium (MFM) (Table 5) was sterile filtered and mixed with autoclaved agar without activated charcoal. As a precaution, activated charcoal was added on top of the agar surface.





It is interesting that they use activated charcoal in order to adsorb toxic compounds in autoclaved agar. But this sort of agars use a lot of different chemical compounds and I think it must be very hard to obtain them.


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Offlinezeronio
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Re: morels and chanterelles to swap for maitake and hericum? [Re: ragadinks]
    #2296270 - 02/03/04 03:21 AM (17 years, 1 month ago)

It's possible to get the chemicals but they're very expensive and you'd need very accurate measuring equipment to prepare this media.
I bet these cantharelles Eric Danell grew cost thousands of $.

Quote:


Composition of Modified Fries Medium (MFM)
based on Straatsma & van Griensven (1986), Eriksson (1965) and Fries
(1978). Murashige & Skoog medium (MS) from Straatsma et al. (1986).
_____________________________________________________________________
MFM MS

D (+) glucose 2.20 g 0
D (-) fructose 2.00 g 0
Sucrose 0 20 g
NH4Cl 0.58 g 0
Succinic acid 0.59 g 0
Meso-inosit 10 mg 100 mg
Macrostock 1a 50 ml 0
Macrostock 1b 0 ml 100 ml
Eriksson microstock2 1 ml 1 ml
Fries vitamine stock3 5 ml 5 ml

Total volume 1000 ml pH 5.5 1000 ml pH 4.2

Composition of macrostock (g/1000 ml):
1a:
KH2PO4 4.00
MgSO4 7H2O 2.00
NaCl 0.40
CaCl2 2H2O 0.52

1b:
KH2PO4 1.7
MgSO4 7H2O 3.7
CaCl2 2H2O 4.4
NH4NO3 16.5
KNO3 19.0

2 Eriksson microstock (200 ml):

EDTA Titriplex III 1.90 g
FeSO4 7H2O 1.40 g

MnSO4 H2O 170 mg
H3BO3 63 mg
ZnSO4 7H2O 287 mg
KI 75 mg
NaMoO4 2H2O 2.50 mg
CuSO4 5H2O 0.25 mg
CoCl2 6H2O 0.25 mg

3 Fries vitaminestock (mg/250 ml):

Thiamine 5.0
Pyridoxine 5.0
Riboflavine 5.0
Biotine 1.25
Nicotinamide 5.0
P-aminobenzoic acid 5.0
Panthotenic acid 5.0





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OfflineBasidiocarp
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Re: morels and chanterelles to swap for maitake and hericum? [Re: zeronio]
    #2296284 - 02/03/04 03:26 AM (17 years, 1 month ago)

Prepared/dehydrated minimal media such as MS can be had from any number of biological supply houses for much cheaper than it would cost to buy all of the constituent ingredients and make it up yourself. A few liters of MS media can be less than twenty US dollars for example. Sigma chemical, Carolina Biological, and Fisher Scientific come to mind. (Sorry to international folks, these are all US firms, as I am a US person.)


--------------------
"...if the mind is actually part of a continuum, a labyrinth that is connected not only to every other mind that exists or has existed, but to every atom, organism, and region in the vastness of space and time itself, the fact that it is able to occasionally make forays into the labyrinth and have transpersonal experiences no longer seems so strange."

Visit the Psychonautical Society


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Re: morels and chanterelles to swap for maitake and hericum? [Re: Basidiocarp]
    #2296292 - 02/03/04 03:30 AM (17 years, 1 month ago)

20 $ ? So there is hope :wink:

What is the difference between PDYA and MF for example. Do you think the chanterelles would not grow on PDYA prepared with active characoal and some antibiotics ?


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Offlinezeronio
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Re: morels and chanterelles to swap for maitake and hericum? [Re: ragadinks]
    #2296314 - 02/03/04 03:46 AM (17 years, 1 month ago)

I think that microelements and vitamins are crucial for success, as well as exact pH. I bet they must have done a lot of trials and failures to get the above formulas.


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Offlinefalcon
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Re: morels and chanterelles to swap for maitake and hericum? [Re: ragadinks]
    #2302393 - 02/04/04 08:34 PM (17 years, 1 month ago)

I'm going to try it with some simple agar recipe, but the activated charcoal sounds like a must. They are just trace minerals and what looks like vitamins, there are probably other places to find these.


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Offlinefalcon
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Re: morels and chanterelles to swap for maitake and hericum? [Re: Basidiocarp]
    #2880084 - 07/12/04 12:01 AM (16 years, 7 months ago)

Chantrelles cloned and grew pretty easily on PDYA agar.  There may be hidden bacterrial contamination. From what I've read it's pretty common with chantrelles.

Aminita muscaria also seems to take well to PDYA. Grown from button.

B. edulis grows well on PDYA and Malt agar.

I've also clone an unknown bolete and moved it onto bird seed and then straw.
Getting it to fruit is probably another matter entirely.

Mycorrhyzal mushrooms do not seem to be that picky about what they will grow on. Must be those idaho potatos :smirk:.

I think one trick to getting them started is using a large chunk, about 1/2 again as big as erasor size and rolling it around on the agar. The first time i put it on agar I did this accidentally and the mycelliun grew from where the mushroom peice had rolled on the agar. It may be that large peices of mushroom fruit inhibit vegetative growth in mycorrhyzal species and small fragments left on the agar are  not  as adverse to growing.


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Offlinefalcon
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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3132110 - 09/13/04 11:07 PM (16 years, 5 months ago)

I transferred the chantrelle culture to brown rice, quarter cup of brown rice to 100 ml of water and pressure cooked. This was done on 8/8 it took about three and a half weeks to colonize completely. I purposely kept the water content low to favor fungal growth.

Today i got up the courage to try it. I scooped the cake out of the jar and added it to a cup and half of boiling water. It has hardly any mushroom flavor and a mild mushroom scent , that drew a mushroom fly to fiery death as it cooked, that is almost exactly like Cambells condensed mushroom soup.
There is none of the fruity scent that you get with Chantrelles though. Edible but not really worth the effort.


I move the B edulis to grain, birdseed, and it took well 4 out 5 jars colonized in about 2 weeks.I then moved it to staw and cotton seed hulls. It colonized fairly quickly and just as quickly succumbed to trich. The unknown bolete is much more resistant to contamination on straw.

The unknown bolete i transferred from agar to supplemented potting soil, sterilized (vermiclulite, peat moss, leaf mold and potato broth). When the potting soil was fully colonized I transfered seedlings of the spruce i found them under to the potting soil. There does not seem to be any difference in the growth of the seedlings in the colonized potting soil and those that had not been transplanted.

What was really cool was getting the spruce to sprout. You never see spruce seedlings in this area. I put the seeds in a plastic bag with damp clean sand and left them in the refrigerator for a month from the middle of April to the middle of May. This cold treatment is called stratifying. I planted them in trays of peat moss. They took from 2 to 3 weeks to sprout.


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3133085 - 09/14/04 03:35 AM (16 years, 5 months ago)

Great experiment !
I cannot believe that you could get the mycelium of chanterelle and boletes to grow on agar !

> think one trick to getting them started is using a large chunk, about 1/2 again as big as erasor size and rolling it around on the agar.
> The first time i put it on agar I did this accidentally and the mycellium grew from where the mushroom piece had rolled on the agar.
> It may be that large peices of mushroom fruit inhibit vegetative growth in mycorrhyzal species and small fragments left on the agar are not as adverse to growing.
Yeah, I experinced the same when I cloned some Calvatia gigantea. Only the bigger peaces took on the agar - the smaller all dried out.
But it is not always that easy to get a big sterile piece of tissue as it is with Calvatia gigantea :wink:.

Have you got some pics of your experiments ?
Would be really interesting to see how the stuff looks like ...


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Offlinefalcon
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Re: morels and chanterelles to swap for maitake and hericum? [Re: ragadinks]
    #3136885 - 09/14/04 11:34 PM (16 years, 5 months ago)

I only have pictures of the fully colonized rice and agar for the chantrelle. 

I have some jars of agar with the boletes still on them. I have one jar of B. Edulis on birdseed, the one  that did not colonize fully. I will take some pictures of these. The bolete I'm not sure what it is, the mycellium starts out white and turns tan over time. Tan was the color of the cap.  It may be a while till I get the picture up though.

Yeah, I experinced the same when I cloned some Calvatia gigantea. Only the bigger peaces took on the agar - the smaller all dried out.
But it is not always that easy to get a big sterile piece of tissue as it is with Calvatia gigantea . :wink:  How is the Calvatia gigantea growing?

I was very lucky to get such a clean chantrelle. When they first come out here in the late spring early summer they are very free from insects for about 2 days. When I went back the next day to collect more for the table they were tunnelled though with larva, still edible, but there is no way i would have had a clean piece to clone.Edit March 26th 2007 the jagged edged blade is not necessary to get a good clone from a Chantrelle, I cloned a chanterelle from a fruit body using a sharp scalpel in June of 2005



My first attempt at B. edulis failed. The other bolete I did at the same time grew without contaminating. We have had a very wet year and the B. Edulis has fruited almost every other week this year.  The second time I tried I used a button.



The mycellium didn't start out of the chunk. It grew from where the chunk had hit the surface of the agar and rolled around. The chunk would get fuzzy but would not grow until the mycellium grew from the agar into the chunk.  I use an exacto knife for a scalpal
I had not changed the blade for a while when I cut the boletes for clones. The Knife edge was rough. I think it left very small peices on the larger chunk that went on to the agar. When the chunk rolled on the agar it left some of those small peices where it had been. Those are what grew, I think.


Edited by falcon (03/26/07 08:07 PM)


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3137391 - 09/15/04 02:32 AM (16 years, 5 months ago)

Looking forward to see the pics of your experiments ...

I really wondered that you were able to clone this species -
on the site were they describe how they cultivated the chanterelles they used very complicated agar formulas in order to get a clean culture.


It is also very interesting that not the big chunk took on the agar but the places were you have rolled that chunk on.
Somehow I have no good explanation for this.
What do you think why this happens ?

> How is the Calvatia gigantea growing?
It does not grow very fast but it grows ...
Here is an older pic - now the mycelium has grown quite a bit:



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Re: morels and chanterelles to swap for maitake and hericum? [Re: ragadinks]
    #3152645 - 09/19/04 12:00 AM (16 years, 5 months ago)



Chanterelles on brown rice.



Chanterelles and unknown bolete on agar



B. edulis on agar and birdseed.










Edited by falcon (09/19/04 12:15 AM)


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3155349 - 09/20/04 12:10 PM (16 years, 5 months ago)

Woow, and the mycelium looks very healthy !
Still cannot believe it !
Keep us updated - that's really going to a very interesting experiment !


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Re: morels and chanterelles to swap for maitake and hericum? [Re: ragadinks]
    #3157157 - 09/20/04 07:17 PM (16 years, 5 months ago)



Pictures of spruce seedlings. The ones in the larger pots have been transplanted to the first bolete I cultured, I don't know what it is.

The ones in the small pots I was going to put into potting soil with B. edulis culture. I didn't get it done this summer. I will put these soon in a sheltered place for the winter.

Hopefully I can keep the B. edulis culture alive until the spring. I plan to buy some two or three year old trees and plant them with colonized soil or grain added to the hole. I've been told that with each successive transfer B edulis loses vitality and finally dies.


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3183360 - 09/26/04 06:32 PM (16 years, 5 months ago)



This is the B. edulis I cloned, a button and one that was hit by a lawn mower.
Ther are also some horse mushrooms.


Edited by falcon (09/26/04 11:02 PM)


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3183396 - 09/26/04 06:48 PM (16 years, 5 months ago)

horse mushroom ? are that the white ones ? they look like agaricus ?


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Re: morels and chanterelles to swap for maitake and hericum? [Re: ragadinks]
    #3183411 - 09/26/04 06:54 PM (16 years, 5 months ago)

They are, Agaricus arvensis. Horse mushroom is the common name.


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Re: morels and chanterelles to swap for maitake and hericum? [Re: ragadinks]
    #3183611 - 09/26/04 08:23 PM (16 years, 5 months ago)

yes, the horse mushrooms are an Agaricus... and they are the white ones in the pic


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Re: morels and chanterelles to swap for maitake and hericum? [Re: Psilygirl]
    #3183618 - 09/26/04 08:26 PM (16 years, 5 months ago)

They really look nice - take a print of them and send me one :wink:
I would like to trade for one ...


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Re: morels and chanterelles to swap for maitake and hericum? [Re: ragadinks]
    #3183638 - 09/26/04 08:31 PM (16 years, 5 months ago)

they were fruiting on a lawn nearby my house a month back, but i havent checked... if i get a chance i'll swing by and get a print.

i havent tried them, personally.


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Re: morels and chanterelles to swap for maitake and hericum? [Re: Psilygirl]
    #3184939 - 09/27/04 02:18 AM (16 years, 5 months ago)

great ! thanks !


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3431616 - 12/01/04 06:30 AM (16 years, 3 months ago)

Your culture of b. edulis is exiting, but... how can you be sure that the "white growing" is not a mold ? :wink:


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Re: morels and chanterelles to swap for maitake and hericum? [Re: snoopline]
    #3434716 - 12/01/04 09:17 PM (16 years, 3 months ago)

I can't.
It behaves very much like A bisporus mycellium.

I think the first clone i did of edulis in June is harbor-
ring bacteria. When it sits on grain after it's been
colonized a while it don't smell right.

I started another
culture in October from button
in the picture.

I have had mold as a contaminate in some of the
jars of mycorrhyzal mushrooms I have cloned.
They seem to put up much more of a fight
against contaminating molds than other mushrooms.

They will climb over and cover the
contaminate.


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3441935 - 12/03/04 12:29 PM (16 years, 3 months ago)

You could use a microscope to differanciate mold and basidiomyc?tes.
Got any one ?


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Re: morels and chanterelles to swap for maitake and hericum? [Re: snoopline]
    #3443446 - 12/03/04 07:53 PM (16 years, 3 months ago)

Yes I have one.

How will I differentiate them with a microscope?


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3444563 - 12/04/04 03:39 AM (16 years, 3 months ago)

Basidiomycetes have some special junction between segments : some kind of inflation (pic1). The junction between segment is "regular" ie smooth with mould


Edited by snoopline (12/04/04 12:27 PM)


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Re: morels and chanterelles to swap for maitake and hericum? [Re: snoopline]
    #3444567 - 12/04/04 03:40 AM (16 years, 3 months ago)

I mean "The junction between segment is "regular" ie smooth with mould"


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Re: morels and chanterelles to swap for maitake and hericum? [Re: snoopline]
    #3445979 - 12/04/04 03:02 PM (16 years, 3 months ago)

Boletus edulis mycellium will not have clamp connections.

Quote:


Tube trama hyaline in KOH, obscurely divergent to subparallel, hyphae 4-7 ?m wide. Pileus trama homogeneous, loosely interwoven, hyaline, hyphae 5-7 ?m wide. Pileus cuticle differentiated as a tangled trichodermium, which collapses with age, occasional free hyphal tips; hyphae 5-7 ?m wide. Stipe cuticle differentiated as a layer of fertile basidia with scattered-to-numerous fusoid-ventricose caulocystidia. Clamp connections absent.




This is describing the fruiting body so I'll look around and see
if I can fin out a description of the mycellium.

I will take a look at the mycellium either way.

Boletus edulis microscopic

From this site graphic of clamp connections , this site calls them septa
connections. Septa are the walls that seperate the cells in the
hypha.

There are some chemical tests at the bottom that may
help seperate it from mold.




Edited by falcon (12/04/04 03:27 PM)


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3502329 - 12/15/04 05:43 AM (16 years, 2 months ago)

So ?
Did you take pictures of the septa of your in vitro's boletus edulis culture ?

Also : do you add any antibiotic to your culture ?


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Re: morels and chanterelles to swap for maitake and hericum? [Re: snoopline]
    #3506391 - 12/15/04 10:13 PM (16 years, 2 months ago)

No not yet. When I transfer it I will.
I don't ussually use antibiotics. I will transfer some of
this to antibiotic agar. I bought some to
to try to get stuff started from wild spores.

This looks like a good time to use it though.


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Re: morels and chanterelles to swap for maitake and hericum? [Re: snoopline]
    #3697694 - 01/29/05 10:47 AM (16 years, 1 month ago)





B. edulis mycellium skimmed off agar at 400X


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3721722 - 02/02/05 10:28 PM (16 years, 1 month ago)

Looking great, Falcon.  Very inspring :thumbup:  Thanks for sharing.


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Re: morels and chanterelles to swap for maitake and hericum? [Re: Yummymush]
    #3732053 - 02/04/05 08:06 PM (16 years, 1 month ago)

Thanks, Yummymush.


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Re: morels and chanterelles to swap for maitake and hericum? [Re: ucsdmolpath]
    #4086756 - 04/22/05 11:23 PM (15 years, 10 months ago)

Hey there ucsdmolpath. I'm a newbie here looking for similar info. I've succesfully put up several types of spawn, but haven't gone toward fruiting yet, suppose I'm just seeing how the morels "act" in culture. There are many accounts, as you must know, of variations of Ower's patent. What hasn't been investigated is the triggering of the fruiting formation. just speculation based on environmental cues, but I'd suspect that there may be biological triggers, like tree death, and other disturbance (fire included) which cause trophic shifts in the soil. Do you have cultures established? What I'm working on may fall under 'rufobrunneus' or the older classifications of yellow strains. I want to get ahold of the blacks in culture, maybe we can trade? let me know.


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #4163578 - 05/11/05 08:45 PM (15 years, 9 months ago)




Quote:

Pictures of spruce seedlings. The ones in the larger pots have been transplanted to the first bolete I cultured, I don't know what it is.

The ones in the small pots I was going to put into potting soil with B. edulis culture. I didn't get it done this summer. I will put these soon in a sheltered place for the winter.

Hopefully I can keep the B. edulis culture alive until the spring. I plan to buy some two or three year old trees and plant them with colonized soil or grain added to the hole. I've been told that with each successive transfer B edulis loses vitality and finally dies




I bought 50 Black Spruce and planted about 45 of them with B. edulis spawn.
The spawn was grown on fine Red Oak sawdust mixed with used tea leaves.
B. edulis grows well on this medium. It also really likes used coffee grounds.
I put about an ounce of sawdust in each hole.

I planted most of them in the ground in fairly wet places. I put a few in
pots.





The trees I grew and found the mushroom under are problably not Black Spruce.
This is the trees at the top of the page.
The Black Spruce I purchased have much lighter colored needles.

I also planted some Blue Oak acorns and added some chantelle spawn to the pots.

Edit: March 26th 2007, the tree I found the Boletus subcearulescens under was some sort of Hemlock I'm not sure which one. I planted 50 Black Spruce trees with Boletus subcearulescens I think about 12 survived, I have two in pots, five in a close ring and 4 or 5 here and there. Two of the trees that were planted were planted without sawdust spawn. Most of the trees that died were planted in too wet soil or in too much shade.



Edited by falcon (03/26/07 08:11 PM)


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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #14001041 - 02/21/11 01:29 PM (10 years, 14 days ago)

Sorry to revive a 5 year old thread, but did this ever end up amounting to anything?


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Re: morels and chanterelles to swap for maitake and hericum? [Re: JSU]
    #14003038 - 02/21/11 07:15 PM (10 years, 14 days ago)

nope, but some of the trees are still around so it wasn't a total loss


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