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Offlinesnoopline
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Registered: 12/01/04
Posts: 5
Last seen: 15 years, 11 months
Re: morels and chanterelles to swap for maitake and hericum? [Re: snoopline]
    #3444567 - 12/04/04 04:40 AM (16 years, 3 months ago)

I mean "The junction between segment is "regular" ie smooth with mould"


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Offlinefalcon
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Registered: 04/01/02
Posts: 7,604
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Re: morels and chanterelles to swap for maitake and hericum? [Re: snoopline]
    #3445979 - 12/04/04 04:02 PM (16 years, 3 months ago)

Boletus edulis mycellium will not have clamp connections.

Quote:


Tube trama hyaline in KOH, obscurely divergent to subparallel, hyphae 4-7 ?m wide. Pileus trama homogeneous, loosely interwoven, hyaline, hyphae 5-7 ?m wide. Pileus cuticle differentiated as a tangled trichodermium, which collapses with age, occasional free hyphal tips; hyphae 5-7 ?m wide. Stipe cuticle differentiated as a layer of fertile basidia with scattered-to-numerous fusoid-ventricose caulocystidia. Clamp connections absent.




This is describing the fruiting body so I'll look around and see
if I can fin out a description of the mycellium.

I will take a look at the mycellium either way.

Boletus edulis microscopic

From this site graphic of clamp connections , this site calls them septa
connections. Septa are the walls that seperate the cells in the
hypha.

There are some chemical tests at the bottom that may
help seperate it from mold.




Edited by falcon (12/04/04 04:27 PM)


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Offlinesnoopline
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Registered: 12/01/04
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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3502329 - 12/15/04 06:43 AM (16 years, 2 months ago)

So ?
Did you take pictures of the septa of your in vitro's boletus edulis culture ?

Also : do you add any antibiotic to your culture ?


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Offlinefalcon
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Re: morels and chanterelles to swap for maitake and hericum? [Re: snoopline]
    #3506391 - 12/15/04 11:13 PM (16 years, 2 months ago)

No not yet. When I transfer it I will.
I don't ussually use antibiotics. I will transfer some of
this to antibiotic agar. I bought some to
to try to get stuff started from wild spores.

This looks like a good time to use it though.


--------------------
Keep growing.


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Offlinefalcon
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Re: morels and chanterelles to swap for maitake and hericum? [Re: snoopline]
    #3697694 - 01/29/05 11:47 AM (16 years, 1 month ago)





B. edulis mycellium skimmed off agar at 400X


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OfflineYummymush
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Registered: 11/17/04
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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #3721722 - 02/02/05 11:28 PM (16 years, 1 month ago)

Looking great, Falcon.  Very inspring :thumbup:  Thanks for sharing.


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Offlinefalcon
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Re: morels and chanterelles to swap for maitake and hericum? [Re: Yummymush]
    #3732053 - 02/04/05 09:06 PM (16 years, 1 month ago)

Thanks, Yummymush.


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Invisiblemushrx1
rainy season
 User Gallery
Registered: 04/22/05
Posts: 80
Re: morels and chanterelles to swap for maitake and hericum? [Re: ucsdmolpath]
    #4086756 - 04/23/05 12:23 AM (15 years, 10 months ago)

Hey there ucsdmolpath. I'm a newbie here looking for similar info. I've succesfully put up several types of spawn, but haven't gone toward fruiting yet, suppose I'm just seeing how the morels "act" in culture. There are many accounts, as you must know, of variations of Ower's patent. What hasn't been investigated is the triggering of the fruiting formation. just speculation based on environmental cues, but I'd suspect that there may be biological triggers, like tree death, and other disturbance (fire included) which cause trophic shifts in the soil. Do you have cultures established? What I'm working on may fall under 'rufobrunneus' or the older classifications of yellow strains. I want to get ahold of the blacks in culture, maybe we can trade? let me know.


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Offlinefalcon
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Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #4163578 - 05/11/05 09:45 PM (15 years, 9 months ago)




Quote:

Pictures of spruce seedlings. The ones in the larger pots have been transplanted to the first bolete I cultured, I don't know what it is.

The ones in the small pots I was going to put into potting soil with B. edulis culture. I didn't get it done this summer. I will put these soon in a sheltered place for the winter.

Hopefully I can keep the B. edulis culture alive until the spring. I plan to buy some two or three year old trees and plant them with colonized soil or grain added to the hole. I've been told that with each successive transfer B edulis loses vitality and finally dies




I bought 50 Black Spruce and planted about 45 of them with B. edulis spawn.
The spawn was grown on fine Red Oak sawdust mixed with used tea leaves.
B. edulis grows well on this medium. It also really likes used coffee grounds.
I put about an ounce of sawdust in each hole.

I planted most of them in the ground in fairly wet places. I put a few in
pots.





The trees I grew and found the mushroom under are problably not Black Spruce.
This is the trees at the top of the page.
The Black Spruce I purchased have much lighter colored needles.

I also planted some Blue Oak acorns and added some chantelle spawn to the pots.

Edit: March 26th 2007, the tree I found the Boletus subcearulescens under was some sort of Hemlock I'm not sure which one. I planted 50 Black Spruce trees with Boletus subcearulescens I think about 12 survived, I have two in pots, five in a close ring and 4 or 5 here and there. Two of the trees that were planted were planted without sawdust spawn. Most of the trees that died were planted in too wet soil or in too much shade.



Edited by falcon (03/26/07 09:11 PM)


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OfflineJSU
Biochem wiz
Male
Registered: 01/10/11
Posts: 102
Last seen: 8 years, 2 months
Re: morels and chanterelles to swap for maitake and hericum? [Re: falcon]
    #14001041 - 02/21/11 02:29 PM (10 years, 16 days ago)

Sorry to revive a 5 year old thread, but did this ever end up amounting to anything?


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Offlinefalcon
In the green


Registered: 04/01/02
Posts: 7,604
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Re: morels and chanterelles to swap for maitake and hericum? [Re: JSU]
    #14003038 - 02/21/11 08:15 PM (10 years, 16 days ago)

nope, but some of the trees are still around so it wasn't a total loss


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Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Amazon Agar, Microscope

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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