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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy

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Re: Ask Quick Questions, Get Quick Answers [Re: Mateja]
    #29628657 -

idk if I can get 12 or 14g at the pharmacy where I live so Ill probably have to order some stuff online, so im looking for ideas from growers that have experience with aspirating LC into syringes :typing:


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Cakes inside Water Tub with optimal Surface Conditions

Edited by Mateja (07/21/26 12:26 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: JW123]
    #29628660 -

It was closer to 75% by the time I was able to get to it.


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Agar stuff

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Re: Ask Quick Questions, Get Quick Answers [Re: Mateja]
    #29628665 -

the higher number Ga the smaller the bore/diameter. 10 Ga is wider than 18 Ga.

I find 18 Ga to get all cloggy and are harder to work with. Typically 16 Ga would be the smallest I would use for anything free flowing.

14 is that sweet spot. 12 if you are working fast with volume and repetitively, like with 60 mL syringes.


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Question everything, it's very responsive.


Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Mateja] * 1
    #29628668 -

Mateja said:
idk if I can get 12 or 14g at the pharmacy where I live so Ill probably have to order some stuff online, so im looking for ideas from growers that have experience with aspirating LC into syringes :typing:



You can get some in that size sometimes from tractor supply stores


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #29628670 -

Hobby shops too for glue dispensing.


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Question everything, it's very responsive.


Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29628673 -

thanks for the ideas, this is what I needed :mushroom2::heart:


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Cakes inside Water Tub with optimal Surface Conditions

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Re: Ask Quick Questions, Get Quick Answers [Re: Mateja]
    #29628680 -

Mateja said:
thanks for the ideas, this is what I needed :mushroom2::heart:



Oh dude, my apologies, I thought you messing around a bit when I saw your post count
:ohshitno:


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Mycology is my current hyperfixation
Uncommunicated expectations are just premeditated resentments.

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29628683 -

Nichrome said:
Hobby shops too for glue dispensing.



I got a bunch of these I picked up on ebay years ago, idk how wide they are but probably way below 12-14G the only thing is they are not sharp/pointed but I guess I could try introducing them to a pair of stainless steele scizzors (or however the hell you spell that, im on the compute) imma give it a go and experiment and report back



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Cakes inside Water Tub with optimal Surface Conditions

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Re: Ask Quick Questions, Get Quick Answers [Re: Mateja]
    #29628684 -

Normaly the color base of the needle should give you the size. Check with google if it matches yours.


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wow

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Re: Ask Quick Questions, Get Quick Answers [Re: Mistakes] * 1
    #29628685 -

Mistakes said:
Mateja said:
thanks for the ideas, this is what I needed :mushroom2::heart:



Oh dude, my apologies, I thought you messing around a bit when I saw your post count
:ohshitno:



I NEVER mess around when it comes to mushroom related science projects, other than that, its all just messing around, and probably too much too


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Cakes inside Water Tub with optimal Surface Conditions

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical]
    #29628686 -

Tropical said:
Normaly the color base of the needle should give you the size. Check with google if it matches yours.



I asked googles AI for the "dark green" needle but it keeps referring me to " ISO standards for standard hypodermic injection needles" and says the're 21G but obviously this is not a 21 nor a medical needle lol

id say its at least 2.5 - 3mm wide


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Cakes inside Water Tub with optimal Surface Conditions

Edited by Mateja (07/21/26 01:00 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Mateja]
    #29628693 -

32 gauge works great for josex poke to inoculate lc's too potentially increasing ur chance at clean lc 😎


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An enigma some caerulescens n a mono 😎

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Re: Ask Quick Questions, Get Quick Answers [Re: Mateja]
    #29628750 -

Mateja said:
is/has anyone here been using LC with syringes succesfully consistently? Im aware about pouring LC's but im specifically interested in injecting (for now) id like to hear some success stories, links, teks, whatever, I feel the need to get motivated somehow, is what im asking, if thats even a question. Who Injects grain with LC around here? How they do it? :shrug:



+1 for 16ga being about the minimum, and 14 or larger being preferable. 18+ is tough.

Mateja said:
I got a bunch of these I picked up on ebay years ago, idk how wide they are but probably way below 12-14G the only thing is they are not sharp/pointed but I guess I could try introducing them to a pair of stainless steele scizzors (or however the hell you spell that, im on the compute) imma give it a go and experiment and report back





Those look like the ones I used for these lids. You can just hook the syringe on and suck it right up. They're way off to the side so the stir bar fits. I keep them plugged with a syringe filter when not in use. Works a treat, recommend.


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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29628889 -

Everyones go to petri dish size ?
3 vent or non vented ?

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Re: Ask Quick Questions, Get Quick Answers [Re: HairyBogan]
    #29628901 -

HairyBogan said:
Everyones go to petri dish size ?
3 vent or non vented ?



90 and 55mm, non vent, but parafilm breathes :rockon:


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Rasmodius] * 1
    #29628956 -

Rasmodius said:
Those look like the ones I used for these lids. You can just hook the syringe on and suck it right up. They're way off to the side so the stir bar fits. I keep them plugged with a syringe filter when not in use. Works a treat, recommend.





yeeeeeees this is the kind of imagery that keeps flashing in my brain, now I have a purpose and a goal, cheers a ton :takingnotes:


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Cakes inside Water Tub with optimal Surface Conditions

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29628965 -

Kloakk said:
HairyBogan said:
Everyones go to petri dish size ?
3 vent or non vented ?



90 and 55mm, non vent, but parafilm breathes :rockon:



No

90 mm non vented as well.

1000 ml makes 30 easily and you get an agro clean culture going and takes like 4 days to eat a plate up and nice quarter wedges dropped to millet is life

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29628984 -

I used 90mm but I went with vented. I used to use the tri section ones but eventually just went to singles. I found the sections too small to work with for transfers and ended up just using them as inoculation plates. But I also had switched to LC as my primary expansion medium and just stopped needing them altogether.

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29628995 -

Splittinghairs said:
Kloakk said:
HairyBogan said:
Everyones go to petri dish size ?
3 vent or non vented ?



90 and 55mm, non vent, but parafilm breathes :rockon:



No

90 mm non vented as well.

1000 ml makes 30 easily and you get an agro clean culture going and takes like 4 days to eat a plate up and nice quarter wedges dropped to millet is life



What do you mean by no 👀


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: HairyBogan]
    #29629061 -

HairyBogan said:
Everyones go to petri dish size ?
3 vent or non vented ?



90mm vented (don't care though), case of 500 is cheap.


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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy


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