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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: idontknow1] * 4
    #29470424 -


Freshly poured agar with a few drops of orange dye added :crazy2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: shroomerhumor] * 11
    #29471346 -

Some nice pan germ plates



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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster] * 1
    #29471498 -

Nice printing setup you got there faht!
:excuseme:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Peleus] * 7
    #29471565 -

Grain is coming soon...Caerulescens.



--------------------
Shrumonoids

Panaeolus Cambodginiensis from Russia
Panaeolus Cyanescens Jamaica from Russia
Goliath.A new greenhouse.Customization

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Re: AGAR ENVY! (Anything and All things agar!) [Re: angamshroom] * 1
    #29471619 -

angamshroom said:
Grain is coming soon...Caerulescens.




That plate sure is purty.


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nokumana] * 2
    #29471858 -

Good morning!

I am looking for a bit of advice please, I'm finding it very hard to get proper growth on my plates.

They are 2% LME / 2% Agar and I left them for a week before using to make sure they were good.

This is my original plate which is x7x+, from LC i think. Ignore the black bits around the edge, just remnants of a previous charcoal agar which the dishwasher missed and I just re-sterilised ..



This is a larger pin taken from the above plate: there is a some rhizo growth which appears to stall and then what I describe as a "cloud" around it which up to now I've been trying to convince myself is very fine tomentose growth.



This one is a much smaller pin - slight bit of rhizo, plenty of "cloud"



I have also done agar transfers from the same original plate and they all end up looking "cloudy" with no rhizo growth.

My questions are:

- is this "cloud" actually tomentose growth or could it be something else? There are no other bacterial signs ..

- should I be making the agar 0% or just 1% LME to promote rhizo growth?

- should i try a different agar brand?

- is everything ok and i just need to chill? :uhoh:

Any tips gratefully received 🙏 🙏 🙏

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Re: AGAR ENVY! (Anything and All things agar!) [Re: McMyco] * 2
    #29471864 -

My first impression is everything is probably fine and you're overthinking it a bit. :smile: Can you get a picture that's well-lit but not from the back?

Less malt extract can encourage more rhizomorphic growth, but it's not a guarantee. And tomentose mycelium is fine too - you're just looking for consistent, uniform growth patterns.

I add a little nutritional yeast (0.2%) to my agar media, which for me makes the cultures grow a little "fuller" and whiter. As always, YMMV, and this is really personal preference - whatever makes it easier to distinguish good and suspect growth, or just makes you happier to look at the plates. :smile:


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LAGM 2026 grow log | My library/trade list

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Phaethon] * 10
    #29471873 -

Ochra plate


--------------------
what i've been growing

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Re: AGAR ENVY! (Anything and All things agar!) [Re: arlo.arlo] * 7
    #29471929 -

These two plates are revivals from year plus agar chips. This was two weeks after the dry chips were put to agar and new transfers were taken.

And my first attempt at cabin sequestration.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: JW123] * 8
    #29471984 -



Got a new toy. PE6 mycelium hyphae under the microscope. Still learning how to use it :evil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: shroomerhumor] * 8
    #29472002 -

I just thought this Weza plate was pretty. Sent part of it to a 1/2 pint jar, but I'm glad a snapped a pic first.



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Re: AGAR ENVY! (Anything and All things agar!) [Re: GnomeEra] * 6
    #29473593 -

Cube clone.


--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Scemilope] * 3
    #29473800 -

Finally got back home and nothing on the plates. Either the spores are taking their sweet time given their age or....they no good. :facepalm:

I'll check back this weekend, but if there is still no growth I'm just gonna try a different print. :sad:

Just keeping the thread posted on progress. More waiting....

:nojustno: :waitingpatiently:


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Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: shroomerhumor] * 1
    #29473980 -

I love that im not the only one with parafilm papers and clingfilm everywhere in my working area.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: lainox]
    #29474046 -

How come my plates Arnt all super white and stringy!? Jealous.. I have this  envy you speak of...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: lainox] * 1
    #29474054 -

Grafting tape fixes the Parafilm backing paper waste problem. :lol:

https://www.amazon.com/dp/B0CMPDSRNG

One of the best changes I ever made. Hated peeling the backing paper off Parafilm. (I've tried cling wrap and just hate handling it in general - it only sticks to things I don't actually want it to stick to. Glad it works for other people though.)


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LAGM 2026 grow log | My library/trade list

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Phaethon] * 1
    #29474255 -

How long can you keep plates?

If you keep transferring can you keep genetics indefinitely?

What temperature kills them?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #29474274 -

So this is the place...

Heya people! QQ - how much LC should I be using when streaking on agar? 1 drop, 1 ml? I avoid Google for things like this...


--------------------
Be lucky, may light walk with you.





Edited by conz0r (02/05/26 04:56 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: conz0r] * 3
    #29474290 -

Just a drop is enough.

Don't press on the plunger, gently apply some pressure on the syringe body/cylinder. If you use a syringe that is.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: conz0r] * 4
    #29474292 -

conz0r said:
So this is the place...

Heya people! QQ - how much LC should I be using when streaking on agar? 1 drop, 1 ml? I avoid Google for things like this...


A drop is fine, squeeze the barrel not the plunger :rockon:

@Rotvelta

:highfive:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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