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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112] * 2
    #29209437 -

Take the plin from this plate onto a new one, that's the way cloning works. Ideally the growth from it should be quite even, and since it is the first pin, theoretically it should be a culture of good fruiters.

Potency is not guaranteed with first plins, so maybe don't suddenly put all the eggs in one basket, keep a more MS line from that plate until potency is established.

I think I've shot myself in the foot by trusting plins a few times without questioning anything.

I cloned a plin once but I suppose it was actually this circular mutation. Potent but pain in the ass to harvest.



--------------------
I call them Huskies cause they Mush!


The Official Wild Cubensis Thread
Instant Pot Agar
Gnocchi Water Agar
Let's Talk Cowtek

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Huskies]
    #29209441 -

Huskies said:
Take the plin from this plate onto a new one, that's the way cloning works. Ideally the growth from it should be quite even, and since it is the first pin, theoretically it should be a culture of good fruiters.

Potency is not guaranteed with first plins, so maybe don't suddenly put all the eggs in one basket, keep a more MS line from that plate until potency is established.

I think I've shot myself in the foot by trusting plins a few times without questioning anything.

I cloned a plin once but I suppose it was actually this circular mutation. Potent but pain in the ass to harvest.




So am I just removing the Plin itself with no agar and placing it on new afar? Like a small precision cut?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112] * 1
    #29209488 -

If you have tweezers you can flame them and use them or just stab the plin with your scalpel and place it on the agar.
When you pull the plin off it will leave the agar behind.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #29209508 -

Thanks!!! 🙏

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112] * 2
    #29209615 -

AZ Starman2112 said:
So am I just removing the Plin itself with no agar and placing it on new afar? Like a small precision cut?


Yes.

If you bring mycelium with it, it's not for sure a clone.

I cut off the head and bottom, (head to make sure it doesn't open a little and drop spores).

Has to be done before any plins drop spores of you don't have a clean clone.
Push the head into a clean plate:

Plin pushed into a fresh plate

Here's one that grew out:


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #29209684 -

nektar61 said:
AZ Starman2112 said:
So am I just removing the Plin itself with no agar and placing it on new afar? Like a small precision cut?


Yes.

If you bring mycelium with it, it's not for sure a clone.

I cut off the head and bottom, (head to make sure it doesn't open a little and drop spores).

Has to be done before any plins drop spores of you don't have a clean clone.
Push the head into a clean plate:

Plin pushed into a fresh plate

Here's one that grew out:



Thanks as well! I’ll be attacking this later this evening.

:rockon:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112] * 5
    #29209714 -

I have to account for unsteady hands, so Ill put a plin on a plate, dice my sample and then take that sample to a 2nd plate. Sacrificial plate bc my hands suck.

Ive left the head on the sacrificial plate too, smacked it around so it's gills down, and then considered that an MS plate. 🤷‍♂️

One in the bag is better than two stones.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

Edited by ThurmanMurman (05/06/25 09:36 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ThurmanMurman] * 4
    #29209854 -

Couple of sequesters that are looking pretty good:

First is blue oyster which is being super aggressive and growing right over the slide covers:


Second is psilocybe mexicana (Strain-A):


--------------------
Sundance's helpful links


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ThurmanMurman] * 2
    #29210009 -

ThurmanMurman said:
I have to account for unsteady hands, so Ill put a plin on a plate, dice my sample and then take that sample to a 2nd plate. Sacrificial plate bc my hands suck.


You wild for this one. PCing agar and hot pouring are a pain in the ass to me. Next behind to hydrating and PCing grains

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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
    #29210021 -

CocaineBuffet said:
ThurmanMurman said:
I have to account for unsteady hands, so Ill put a plin on a plate, dice my sample and then take that sample to a 2nd plate. Sacrificial plate bc my hands suck.


You wild for this one. PCing agar and hot pouring are a pain in the ass to me. Next behind to hydrating and PCing grains


I still love HGs man. Easy peasy and I can use them right away.

Ive got 100 autoclavable plastic petris and I used them all once and kinda put them on the shelf until I get off my ass and sterilize them. I even bought those silicone bread pans someone recommended, which work out perfect in holding 12 plates real snug. I really do need to do that..


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ThurmanMurman]
    #29210287 -

Very baccy? T1 TTBVI plates. Is it worth getting a sample from anywhere on these? Pretty aggressive for 7 days though. Yesterday these plates looked different, as in good different.




Edited by AZ Starman2112 (05/07/25 08:06 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bxolotl] * 3
    #29210293 -

LadysKnight said:
Nice plates!
I see this on a few, so I assume camera artifact?





Bxolotl said:
edit: here you can even see the reflexion of the hand holding the mobile…:







You guys were right, it was reflections or something on the lens. Plates looked good when I looked last night.

Edited by AZ Starman2112 (05/07/25 08:07 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112] * 5
    #29210310 -

Old test plate of vendor LC decided it wanted to get stoned :gethigh:


Not sure what they're called. Plones? plerotia? pluffles?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AZ Starman2112] * 3
    #29210380 -

AZ Starman2112 said:
Very baccy? T1 TTBVI plates. Is it worth getting a sample from anywhere on these? Pretty aggressive for 7 days though. Yesterday these plates looked different, as in good different.






12 o cliock in second picture looks good to me. i'd transfer from there, then transfer again asap once you have a nice leading edge on the new plate


--------------------
I call them Huskies cause they Mush!


The Official Wild Cubensis Thread
Instant Pot Agar
Gnocchi Water Agar
Let's Talk Cowtek

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Huskies] * 5
    #29211372 -



--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Huskies] * 1
    #29211403 -

ThurmanMurman said:



:loveeyes:


Huskies said:
AZ Starman2112 said:



12 o cliock in second picture looks good to me. i'd transfer from there, then transfer again asap once you have a nice leading edge on the new plate



That’s what I went for this morning on a transfer. Took two punch outs and put them on new agar plates. Thanks for looking out pardner! 🤠

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ThurmanMurman]
    #29211417 -

ThurmanMurman said:





--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight] * 2
    #29211431 -

My vote is that it's debris on the lid and not the plate


--------------------
Check your vector

Gummies by milkboy
Milkboys Public Display of Affection
I'm a Semp


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Re: AGAR ENVY! (Anything and All things agar!) [Re: milkboy] * 3
    #29211434 -

Me too, but it would be remiss to remain silent.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight] * 4
    #29211580 -

Trying to up my agar game after seeing some great examples of D3's tek, shoutout to AZStarman for the inspiration. These look sendable? Or transfer?

RTOG T2


X7x+ T2


Ochra T3a


Ochra T3b


ESS needs to be transferred again, the T0 plate strains grew together, need to isolate more.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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