Huskies said:

Take the plin from this plate onto a new one, that's the way cloning works. Ideally the growth from it should be quite even, and since it is the first pin, theoretically it should be a culture of good fruiters.

Potency is not guaranteed with first plins, so maybe don't suddenly put all the eggs in one basket, keep a more MS line from that plate until potency is established.

I think I've shot myself in the foot by trusting plins a few times without questioning anything.

I cloned a plin once but I suppose it was actually this circular mutation. Potent but pain in the ass to harvest.

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AZ Starman2112 said:

So am I just removing the Plin itself with no agar and placing it on new afar? Like a small precision cut?

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Yes.

If you bring mycelium with it, it's not for sure a clone.

I cut off the head and bottom, (head to make sure it doesn't open a little and drop spores).

Has to be done before any plins drop spores of you don't have a clean clone.
Push the head into a clean plate:

Plin pushed into a fresh plate

Here's one that grew out:

nektar61 said:

AZ Starman2112 said:

So am I just removing the Plin itself with no agar and placing it on new afar? Like a small precision cut?

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Yes.

If you bring mycelium with it, it's not for sure a clone.

I cut off the head and bottom, (head to make sure it doesn't open a little and drop spores).

Has to be done before any plins drop spores of you don't have a clean clone.
Push the head into a clean plate:

Plin pushed into a fresh plate

Here's one that grew out:

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Thanks as well! I’ll be attacking this later this evening.

ThurmanMurman said:

I have to account for unsteady hands, so Ill put a plin on a plate, dice my sample and then take that sample to a 2nd plate. Sacrificial plate bc my hands suck.

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CocaineBuffet said:

ThurmanMurman said:

I have to account for unsteady hands, so Ill put a plin on a plate, dice my sample and then take that sample to a 2nd plate. Sacrificial plate bc my hands suck.

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You wild for this one. PCing agar and hot pouring are a pain in the ass to me. Next behind to hydrating and PCing grains

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LadysKnight said:

Nice plates!
I see this on a few, so I assume camera artifact?

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Bxolotl said:

edit: here you can even see the reflexion of the hand holding the mobile…:

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AZ Starman2112 said:

Very baccy? T1 TTBVI plates. Is it worth getting a sample from anywhere on these? Pretty aggressive for 7 days though. Yesterday these plates looked different, as in good different.

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ThurmanMurman said:

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Huskies said:

AZ Starman2112 said:

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12 o cliock in second picture looks good to me. i'd transfer from there, then transfer again asap once you have a nice leading edge on the new plate

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ThurmanMurman said:

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