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PhenoDreamers
hi lol

Registered: 09/02/13
Posts: 3,677
Loc: USA

Last seen: 35 minutes, 23 seconds

Re: AGAR ENVY! (Anything and All things agar!)

5
#29169642 - 03/26/25 04:44 PM (1 year, 7 months ago)

Sect zap NZ02 showing some nice growth

@treefrog, bring that culture over next time and we'll check it for clamps! Whatever you do, DONT toss it. Could be gold in your hands 😂

Edited by PhenoDreamers (03/26/25 04:45 PM)

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Noob60

Registered: 06/28/24
Posts: 47
Last seen: 1 year, 3 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: MycCycle]
#29169644 - 03/26/25 04:51 PM (1 year, 7 months ago)

Nice👏🏼

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Noob60

Registered: 06/28/24
Posts: 47
Last seen: 1 year, 3 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: BeefExtreme]
#29169651 - 03/26/25 04:57 PM (1 year, 7 months ago)

WOW💯impressed

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tree frog
sapphic love muffin

Registered: 03/31/14
Posts: 7,011

Re: AGAR ENVY! (Anything and All things agar!)

[Re: fahtster]
#29169655 - 03/26/25 05:04 PM (1 year, 7 months ago)

Yeah, I had actually already started fresh spores before I made that post I think I did it a couple of days ago :lol:

:lol:

I just wasn't sure if I should keep bothering with this culture mostly worrying about senescence. (Which maybe I shouldn't be so worried about).

I'll take it to Supa's too. Would be nice to to see how clean my culture work is, if there's enmeshed bacteria or competitor mold.

--------------------
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And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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PhenoDreamers
hi lol

Registered: 09/02/13
Posts: 3,677
Loc: USA

Last seen: 35 minutes, 23 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tree frog]
#29169733 - 03/26/25 06:32 PM (1 year, 7 months ago)

Still think there's a chance it could be mono. So could check everything out and see what's up

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AZ Starman2112
Male Half of a Friendly Couple

Registered: 12/11/24
Posts: 2,640
Loc: A Steamy Bedroom With The Cool...
Last seen: 1 day, 15 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: PhenoDreamers]

2
#29171346 - 03/28/25 12:17 PM (1 year, 6 months ago)

I inoculated a dozen more plates this morning two plates each of 6 different types. Trying the Pan Cyan PR & AHB’s again. If they contaminate up the same way again, I’m gonna assume I have bad syringes.

I think I had a better work flow and a cleaner one as well this go around. I used the drop method on all of them. Looks like my remaining eight plates from the first attempt are all looking good with maybe the exception of one. I’m gonna continue to let them ride.

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Lilgrower
Untitled

Registered: 08/05/24
Posts: 485

Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]

5
#29171881 - 03/28/25 07:46 PM (1 year, 6 months ago)

I just thought it was interesting the way x7x+ treats food coloring compared to semps. Both cups used to be that green.

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Digit
Surreal Estate Agent

Registered: 08/10/15
Posts: 144
Loc: tierra incognita

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lilgrower]

1
#29172762 - 03/29/25 05:11 PM (1 year, 6 months ago)

So I have two very different apparent phenotypes from a NZ-02 swab.
The first to germ started around a week ahead of the other as a an extremely tiny spot of just a few strands of myc, it seems to grow a LOT faster, starts real thin and wispy looking but eventually thickens up.
The other one looks more like a typical active but is much slower.
Not sure if the faster one is even my target species but I can’t imagine what else it could be.
These plates are the same age, although the one labeled #2 is a T3 on PDYA and the other is a T2 on low nutrient MEA. The difference could possibly be explained by the nutrient content, and the fact that the slower one was from a much smaller sample of myc, but previous transfers on the same type of agar have yielded similar results.
I’m not really sure what to think of these very different morphological characteristics from the same swab. Nonetheless I’ll do at least another round of transfers, possibly with CS before I go to grain but it’s just very strange how different these two cultures are.
Any advice or suggestions would be much appreciated.

--------------------
Question everything.
Believe nothing.
Think for yours3lf.

Edited by Digit (03/29/25 07:23 PM)

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,607
Loc: Hollywoo

Last seen: 43 minutes, 22 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lilgrower]

2
#29173018 - 03/29/25 10:09 PM (1 year, 6 months ago)

Lilgrower said:

I just thought it was interesting the way x7x+ treats food coloring compared to semps. Both cups used to be that green.

Which ones are which?

Some food coloring gets eaten in a weird way that some people think at first is contam. I only see a color change here, and only on one.

Pic tax, Psilo Cyan on my favorite agar "coloring" (activated charcoal, which is actually a nutrient too)

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--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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fungitobewith
Fungi To Be With

Registered: 07/15/07
Posts: 979
Loc: Rolling Stone, PA/Some Place C...

Re: AGAR ENVY! (Anything and All things agar!)

1
#29173683 - 03/30/25 03:51 PM (1 year, 6 months ago)

Looking pretty good

Testing out some wheat water agar plates, mycelium devoured the MEA chunk before it decided it was going to move to the less nutritious WWA.

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Edited by fungitobewith (03/30/25 03:52 PM)

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rhizoRider
Mycorrhizally expanding

Registered: 12/24/13
Posts: 3,530

Re: AGAR ENVY! (Anything and All things agar!)

[Re: fungitobewith]

2
#29173795 - 03/30/25 05:43 PM (1 year, 6 months ago)

Just flying along fast at 60F :awedance:

:awedance:

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sage_bd
humble mushroom farmer

Registered: 12/16/24
Posts: 174
Loc: Stardew Valley

Re: AGAR ENVY! (Anything and All things agar!)

[Re: rhizoRider]

3
#29174029 - 03/31/25 12:04 AM (1 year, 6 months ago)

I've been loving these grain to agar transfers :biggrin:

:biggrin:

This guy kinda looks like he grew wings:

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LewDoja
mad $cientist, ganjacologist

Registered: 09/18/09
Posts: 4,849
Loc: A fictional tale, in fictional...
Last seen: 5 months, 18 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: sage_bd]

1
#29174057 - 03/31/25 12:50 AM (1 year, 6 months ago)

I dig the angle wings on that last plate

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AZ Starman2112
Male Half of a Friendly Couple

Registered: 12/11/24
Posts: 2,640
Loc: A Steamy Bedroom With The Cool...
Last seen: 1 day, 15 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: nektar61]
#29174336 - 03/31/25 11:09 AM (1 year, 6 months ago)

nektar61 said:

Some food coloring gets eaten in a weird way that some people think at first is contam. I only see a color change here, and only on one.

Pic tax, Psilo Cyan on my favorite agar "coloring" (activated charcoal, which is actually a nutrient too)

How do you like using AC for your plates? I have some in capsules and was thinking of making some black plates on my next pour. I made 3 different colors on my first attempt and I’m leaning towards blue as the color I prefer, but was gonna try black as well. I need to make a bunch like stat!

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,607
Loc: Hollywoo

Last seen: 43 minutes, 22 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]
#29174466 - 03/31/25 02:09 PM (1 year, 6 months ago)

AZ Starman2112 said:

nektar61 said:

Some food coloring gets eaten in a weird way that some people think at first is contam. I only see a color change here, and only on one.

Pic tax, Psilo Cyan on my favorite agar "coloring" (activated charcoal, which is actually a nutrient too)

How do you like using AC for your plates? I have some in capsules and was thinking of making some black plates on my next pour. I made 3 different colors on my first attempt and I’m leaning towards blue as the color I prefer, but was gonna try black as well. I need to make a bunch like stat!

I like it a lot. I think they grow better, and I think it makes it easier to see some contam than either food coloring or nothing added.

I didn't do it this time because I ran out and decided on the spot to get back into this after 9 month break, and was making agar plates within an hour.

Here's what I do:
https://www.shroomery.org/forums/showflat.php/Number/27551422#27551422

--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Extras:

Lilgrower
Untitled

Registered: 08/05/24
Posts: 485

Re: AGAR ENVY! (Anything and All things agar!)

2
#29174490 - 03/31/25 03:12 PM (1 year, 6 months ago)

nektar61 said:

Lilgrower said:

I just thought it was interesting the way x7x+ treats food coloring compared to semps. Both cups used to be that green.

Which ones are which?

Some food coloring gets eaten in a weird way that some people think at first is contam. I only see a color change here, and only on one.

Pic tax, Psilo Cyan on my favorite agar "coloring" (activated charcoal, which is actually a nutrient too)

The x7x+ ate the food coloring. The Semps did not at all.

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Pillowtop
MR.

Registered: 02/16/25
Posts: 17
Last seen: 1 year, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lilgrower]

4
#29175341 - 04/01/25 01:31 PM (1 year, 6 months ago)

OK been lurking here too long already. Here are some pics of my first attempt at doing anything with agar. I inoculated (3) plates each of ISS from a trusted vendor, APE, GT, and B+. Took transfers of those plates to 1/2 pint agar jars, won't be doing that again... What a pain in the ass. Took more transfers of the spore plates to 90mm petri dishes. Finally have taken a bunch of transfers from what I'm calling my T1 plates to 60mm T2 petris'.

Before anyone asks, I bought premixed agar powder since it's my first time and it was relatively inexpensive. I also did not intend to purchase the 60mm plates, but alas my 'zon Fu was off that day and what I ended up buying was 60mm plates. The real kicker is 3 days later I attempted to buy 90mm peteis' and AGAIN ordered the 60mm, so now I have 500 60mm petri dishes, hrmm.

Code:

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-Happy Growing-

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Digit
Surreal Estate Agent

Registered: 08/10/15
Posts: 144
Loc: tierra incognita

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Pillowtop]
#29175600 - 04/01/25 06:05 PM (1 year, 6 months ago)

Pillowtop said:

OK been lurking here too long already. Here are some pics of my first attempt at doing anything with agar. I inoculated (3) plates each of ISS from a trusted vendor, APE, GT, and B+. Took transfers of those plates to 1/2 pint agar jars, won't be doing that again... What a pain in the ass. Took more transfers of the spore plates to 90mm petri dishes. Finally have taken a bunch of transfers from what I'm calling my T1 plates to 60mm T2 petris'.

Before anyone asks, I bought premixed agar powder since it's my first time and it was relatively inexpensive. I also did not intend to purchase the 60mm plates, but alas my 'zon Fu was off that day and what I ended up buying was 60mm plates. The real kicker is 3 days later I attempted to buy 90mm peteis' and AGAIN ordered the 60mm, so now I have 500 60mm petri dishes, hrmm.

Code:

Looks good amigo, well done.
60mm plates are fine imho especially if you intend to transfer quickly.

--------------------
Question everything.
Believe nothing.
Think for yours3lf.

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Digit
Surreal Estate Agent

Registered: 08/10/15
Posts: 144
Loc: tierra incognita

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Digit]
#29175605 - 04/01/25 06:11 PM (1 year, 6 months ago)

Looks like a hyphal knot, right?
Should I take a sample to transfer from there? It’s not the thickest part of the culture but if it’s knotting that’s a sign of good fruiting ability, correct?
It’s been over a decade since I did much with agar and my knowledge is getting rusty.

--------------------
Question everything.
Believe nothing.
Think for yours3lf.

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Huskies
Boop More Snoots

Registered: 03/22/16
Posts: 2,782
Last seen: 17 minutes, 34 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Digit]
#29175612 - 04/01/25 06:16 PM (1 year, 6 months ago)

^ I think that is a safe bet, but take a sample from a stronger looking region and also compare how they both grow on new plates.

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