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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: joze]
    #28678432 -

:takingnotes:

What about water agar vs nutrient agar?

What's better for germination?


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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28678436 -

Yahra said:
:takingnotes:

What about water agar vs nutrient agar?

What's better for germination?


I have only ever used one recipe for agar: 10 g agar, 10 g malt extract, 1 g nutritional yeast, in 500 mL water.

That is what I use for everything from germination to cleaning up contaminated cultures.


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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28678515 -

If you have a spore print, youd be much better served using a qtip than your scalpel blade. Streak the plate with a letter Z, let it grow out away from the streak mark, then transfer. You may have to dip the tip of the qtip into the agar first before trying to pick up some spores from the print. Just to moisten it ever so slightly.


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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator] * 2
    #28678542 -

Yahra said:
:takingnotes:

What about water agar vs nutrient agar?

What's better for germination?



I know this doesn't answer your question but I'd say BRF pucks in 4oz jars is your best bet. For spores that won't cooperate BRF pucks are going to save you a lot of time and effort

In my experience the best luck I've had with stubborn spores on agar is to put them onto a swab and dunk the swab head into the agar. That requires a little bit of a thicker pour

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Re: Ask Quick Questions, Get Quick Answers [Re: tholos] * 2
    #28678682 -

tholos said:
Yahra said:
:takingnotes:

What about water agar vs nutrient agar?

What's better for germination?



I know this doesn't answer your question but I'd say BRF pucks in 4oz jars is your best bet. For spores that won't cooperate BRF pucks are going to save you a lot of time and effort

In my experience the best luck I've had with stubborn spores on agar is to put them onto a swab and dunk the swab head into the agar. That requires a little bit of a thicker pour


To answer your question, I only use nutritive agar in my op. Water agar is useful, but I recommend germing on regular agar.

Having said that, BRF pucks are mad useful for germing spores. They work really well.


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #28678718 -

Thanks everyone for the info. I'm hoping genetic as the spawn was super clean. No excess moisture, fully colonized and I let it consolidate for 3 weeks before spawning. I use a ultra fine continuous mister and spray up letting the droplets fall to the surface. No foul smell just a clean fresh cut mushroom smell. I'm going to hold off on misting to see if it helps.


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Re: Ask Quick Questions, Get Quick Answers [Re: Bigworm]
    #28678803 -

Whole Oat Grain Jars

Should I shake either of them to redistribute? And then do I wait for them to be FULLY colonized before mixing into coir? What does that look like? Just super fuzzy white?




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Let us begin living again

Edited by FamiliarFinding384 (02/27/24 09:10 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: FamiliarFinding384]
    #28678814 -

FamiliarFinding384 said:
Whole Oat Grain Jars

Should I shake either of them to redistribute? And then do I wait for them to be FULLY colonized before mixing into coir? What does that look like? Just super fuzzy white?





Shake and break. You don’t want to see any naked grains. The whole jar should be fuzzy, yes. That is not ready to be sent at all.

Uncolonized grains will get colonized by other organisms like bacteria and trichoderma.

That second jar is possibly bacterial as well. Keep an eye on it. If it doesn’t recover after your shake, you may want to toss it


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

Edited by Smellyhobbit (02/27/24 09:25 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28678820 -

Cool - how often should I shake until they are all covered? I'm guessing too often would be counterproductive.


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Let us begin living again

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Re: Ask Quick Questions, Get Quick Answers [Re: FamiliarFinding384]
    #28678826 -

FamiliarFinding384 said:
Cool - how often should I shake until they are all covered? I'm guessing too often would be counterproductive.


Shake it, mix the grains around, and let them recover to the point that they’ve colonized more grain. When your heart tells you to shake, shake. Some people wait longer than others.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: FamiliarFinding384]
    #28678828 -

You should only need to shake twice. Once after about 5-6 days and then at 30%


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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator]
    #28678830 -

I def shake more than that because I’m impatient lol


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28678860 -



are contaminated?


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" Antes de que cambien los reinos, es necesario que cambien los individuos"

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Re: Ask Quick Questions, Get Quick Answers [Re: DRAKENCL]
    #28678879 -

Hello I'm concerned about the water droplets on these plates
I don't think I'll be doing black agar again, it was my first try, will probably try to make my own plates with a TEK.

Some of these plates are from MS to grain, the thread looking ones are my attempt to clone from the stem of my columbian grow. Mush love ❤️




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"Honesty without kindness is brutality, kindness without honesty is manipulation "

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 5
    #28678893 -

Smellyhobbit said:
I def shake more than that because I’m impatient lol


If you shake it more than twice youre playing with it.


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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator] * 1
    #28678897 -

AspectOfTheCreator said:
Smellyhobbit said:
I def shake more than that because I’m impatient lol


If you shake it more than twice youre playing with it.


:rofl:

And I am, too


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Ylunnatic]
    #28678965 -

Everything looks fine except for the 3rd picture. Definitely have more than enough to transfer with to a new dish. Why won’t you use charcoal again? It’s fine. Mycelium is just slower on it.

@DRAKENCL, no. They’re clean

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Re: Ask Quick Questions, Get Quick Answers [Re: PhenoDreamers]
    #28679006 -

Thanks for looking at them ! Yeah :frown: the clones from fruit aren't looking to hot. I really want a medium I can see all the way through, I'm still learning and want to really stare at them.


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"Honesty without kindness is brutality, kindness without honesty is manipulation "

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Re: Ask Quick Questions, Get Quick Answers [Re: Ylunnatic]
    #28679023 -

Mold, or Myc?

(Natalensis spore swab plate)






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Re: Ask Quick Questions, Get Quick Answers [Re: Luminous7]
    #28679076 -

Luminous7 said:
Mold, or Myc?

(Natalensis spore swab plate)







I am willing to bet that it is mold. Let's wait for the more experienced folks though. Oh, may I ask, how long from streaking to germination?


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Ἄνδρα μοι ἔννεπε, Μοῦσα, πολύτροπον

Edited by Skropi (02/28/24 02:04 AM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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